An apparatus (100) for mixing a liquid (160) containing particulates (106, 108) comprising: a vessel (102) for containing the liquid (160) with a sidewall (120) and bottom (124); an impeller (300) rotates about a substantially vertical axis (X-X), the impeller submerging below the liquid surface (162) by a distance approximately one-tenth to one-half of the liquid (129) height; at least two spaced apart blades (310) extending radially outwardly of the vertical axis, the blades including back-swept blades pitched substantially parallel to the vertical axis, at least 50% of the length of each blade comprising an angled section (312) extending through a chord angle of 20 to 60 degrees to produce: an inner, upward flow region (164) along said vertical axis, a transition flow region (166) around the impeller in which liquid moves radially outwardly toward the vessel sidewall, and an outer, downward flow region (168) along the sidewall.

An apparatus (100) for mixing a liquid (160) containing particulates (106, 108) comprising: a vessel (102) for containing the liquid (160) with a sidewall (120) and bottom (124); an impeller (300) rotates about a substantially vertical axis (X-X), the impeller submerging below the liquid surface (162) by a distance approximately one-tenth to one-half of the liquid (129) height; at least two spaced apart blades (310) extending radially outwardly of the vertical axis, the blades including back-swept blades pitched substantially parallel to the vertical axis, at least 50% of the length of each blade comprising an angled section (312) extending through a chord angle of 20 to 60 degrees to produce: an inner, upward flow region (164) along said vertical axis, a transition flow region (166) around the impeller in which liquid moves radially outwardly toward the vessel sidewall, and an outer, downward flow region (168) along the sidewall.

The present application provides a high-gravity device for generating nano/micron bubble and a reaction system. In the device, the liquid phase is continuous phase and the gas phase is dispersed phase. A gas enters the interior of the device from a hollow shaft, and the gas is subjected to primary shearing under a shearing effect of aerating micropores to form bubbles; then, the bubbles rapidly disengage from the surface of a rotating shaft under the effect of the rotating shaft rotating at a high speed, and are subjected to secondary shearing under the high-gravity environment with the strong shearing force formed by the rotating shaft to form nano/micron bubbles. The device has the advantages of fastness, stability, and small average particle size. The average particle size of the formed nano/micron bubbles is between 800 nanometers and 50 microns, and the average particle size of the bubbles can be regulated in a range by adjusting the rotating speed of the rotating shaft.

The present application provides a high-gravity device for generating nano/micron bubble and a reaction system. In the device, the liquid phase is continuous phase and the gas phase is dispersed phase. A gas enters the interior of the device from a hollow shaft, and the gas is subjected to primary shearing under a shearing effect of aerating micropores to form bubbles; then, the bubbles rapidly disengage from the surface of a rotating shaft under the effect of the rotating shaft rotating at a high speed, and are subjected to secondary shearing under the high-gravity environment with the strong shearing force formed by the rotating shaft to form nano/micron bubbles. The device has the advantages of fastness, stability, and small average particle size. The average particle size of the formed nano/micron bubbles is between 800 nanometers and 50 microns, and the average particle size of the bubbles can be regulated in a range by adjusting the rotating speed of the rotating shaft.

A mixing unit includes a mixing body having mixing elements that are stacked in a stacking direction and that extend in an extending direction in which the extending direction is perpendicular to the stacking direction. The mixing elements have a plurality of through holes to form a flow path therein, and are arranged such that part or all of the through holes in one of the mixing elements communicate with through holes in the adjacent mixing elements to allow fluid to be passed in the extending direction in which the mixing elements extend. The mixing unit may be employed in an agitation impeller or an adhesive dispensing unit.

A mixing unit comprising a frame, a rheology control portion, and a high-volume solids blending portion. The rheology control portion comprises means for receiving a first material from a first transfer mechanism, a dispersing/mixing system connected with the frame, and a first metering system to meter the first material from the first material receiving means to the dispersing/mixing system. The dispersing/mixing system disperses/mixes the metered first material with a fluid to form a first fluid mixture. The high-volume solids blending portion comprises means for receiving a second material from a second transfer mechanism, a solids blending system connected with the frame, and a second metering system to meter the second material from the second material receiving means to the solids blending system. The solids blending system blends the metered second material with the first fluid mixture to form a second fluid mixture.

Inside a driven cylindrical housing 23 that is a cylindrical rotation member 13, a liquid to be mixed 4 is caused to become an inner circulation current f by extruded plate portions 24A-24D. Discharge ports 22A-22D formed in the cylindrical housing 21 discharge a portion of the inner circulation current f outward as outer discharge current d1-d4 by centrifugal force. At the same time, the outer portion of the liquid to be mixed 4 is sucked into suction ports 23, 30 as suction current e1-e3, h1-h4, thus mixing the liquid to be mixed 4 in the mixing tank 3.

The present invention concerns a stirrer system for animal cell culture consisting of a combination of at least one radially-conveying stirrer element and at least one axially-conveying stirrer element, wherein at least three stirrer elements must be present and the uppermost stirrer element is an axially-conveying stirrer element. The stirrer elements are arranged at a certain distance above one another on a stirrer shaft. A particular embodiment is a multiple stirrer system consisting of two disk stirrers as radially-conveying stirrer elements and an inclined-blade stirrer as an axially-conveying stirrer element wherein the inclined-blade stirrer is arranged above the disk stirrer on the stirrer shaft. The stirrer system according to the invention achieves among others a gentler and better intermixing in the culture of shear-sensitive mammalian cells in cell cultures.

A flask for containing mold sand within it prevents any mold shifting or mold dropping. Each of an upper flask 2 and a lower flask 3 includes a body that defines an opening in which a sand mold is to be molded. The body has at least one inlet 101 for introducing the mold sand into the opening. Two flanges 102 are extended from the body such that they are opposed to each other across the opening. Each flange has a through bore. The flask also includes engaging members for engaging an actuator in the outside of the flask such that a force or forces from said actuator could be transmitted to the flask. An upper flask 104 and a lower flask 105 are opposed to each other across a pattern plate 107. They are integrally assembled to make a flask unit by means of a pair of connecting rods 106 that are fitted in each bore.

The present invention concerns a stirrer system for animal cell culture consisting of a combination of at least one radially-conveying stirrer element and at least one axially-conveying stirrer element, wherein at least three stirrer elements must be present and the uppermost stirrer element is an axially-conveying stirrer element. The stirrer elements are arranged at a certain distance above one another on a stirrer shaft. A particular embodiment is a multiple stirrer system consisting of two disk stirrers as radially-conveying stirrer elements and an inclined-blade stirrer as an axially-conveying stirrer element wherein the inclined-blade stirrer is arranged above the disk stirrer on the stirrer shaft. The stirrer system according to the invention achieves among others a gentler and better intermixing in the culture of shear-sensitive mammalian cells in cell cultures.