The present disclosure provides methods and systems for assaying a sample. A microfluidic device to perform an assay of a sample (e.g., biological sample) is described having a sample application site, a porous component and a flow channel. The porous component provides for uniform dissolution of a reagent and mixing of the sample and reagent without filtering the sample.

Microstructure flow mixing devices are disclosed herein. An example device a first panel, a first plurality of raised features extending from a first surface of the first panel, a second plurality of raised features extending from the first surface of the first panel and a plurality of divider microstructures extending from the first surface of the first panel in line with and in between the first plurality of raised features and the second plurality of raised features. At least a portion of adjacent divider microstructures are spaced apart to form feed pathways or cross channels.

The present invention provides an improved process for lipid nanoparticle formulation and mRNA encapsulation. In some embodiments, the present invention provides a process of encapsulating messenger RNA (mRNA) in lipid nanoparticles comprising a step of mixing a suspension of preformed lipid nanoparticles and mRNA.

A reagent pre-loading system for a measuring device includes: a reagent admission arrangement arranged for receiving a reagent into the reagent pre-loading system; a sample admission arrangement arranged for receiving a sample fluid to be measured into the reagent pre-loading system, wherein the reagent admission arrangement and the sample admission arrangement are each arranged in selective fluid communication with a sample and reagent combination conduit for combining the sample fluid and the reagent; wherein the sample admission arrangement and the reagent admission arrangement are arranged to selectively: whilst the sample and reagent combination conduit is closed to fluid communication with the sample fluid at the sample admission arrangement, receive the reagent into the sample and reagent combination conduit so as to expel air from the sample and reagent combination conduit as the reagent is received therein and prime the sample and reagent combination conduit with the reagent.

The application relates to microfluidic apparatus and methods of use thereof. Provided in one example is a microfluidic device comprising: a first fluidic input and a second fluidic input; and a fluidic intersection channel to receive fluid from the first fluidic input and the second fluidic input, wherein the fluidic intersection channel opens into a first mixing chamber on an upper region of a first side of the first mixing chamber, wherein the first mixing chamber has a length, a width, and a depth, wherein the depth is greater than about 1.5 times a depth of the fluidic intersection channel; an outlet channel on an upper region of a second side of the first mixing chamber, wherein the outlet channel has a depth that is less than the depth of the first mixing chamber, and wherein an opening of the outlet channel is offset along a width of the second side of the first mixing chamber relative to the fluidic intersection.

A lipid nanoparticles manufacturing chip includes a first raw material supply flow path, a second raw material supply flow path, and a mixer portion connected to the first raw material supply flow path and the second raw material supply flow path and configured for mixing a first raw material supplied through the first raw material supply flow path and a second raw material supplied through the second raw material supply flow path. The mixer portion includes a first stabilizing unit, and a first mixing unit connected to the first stabilizing unit and configured for mixing the first raw material and the second raw material with each other. Mixing of the first raw material and the second raw material is performed more in the first mixing unit than in the first stabilizing unit.

A microfluidic device comprises: a first channel through which a first raw material fluid flows; a second channel through which a second raw material fluid flows; a synthesis channel portion that joins the first channel and the second channel to cause the first raw material fluid and the second raw material fluid to react with each other to generate nuclei, thereby obtaining a fluid containing particles obtained by growing the nuclei; and a vibration applicator that applies vibration to the fluid flowing through the synthesis channel portion, in which the synthesis channel portion includes a mixing channel portion that is a merging portion of the first channel and the second channel, a stagnation channel portion that communicates with a downstream side of the mixing channel portion and to which vibration is applied by the vibration applicator.

A microfluidic device comprises: a first channel through which a first raw material fluid flows; a second channel through which a second raw material fluid flows; a synthesis channel portion that joins the first channel and the second channel to cause the first raw material fluid and the second raw material fluid to react with each other to generate nuclei, thereby obtaining a fluid containing particles obtained by growing the nuclei; and a vibration applicator that applies vibration to the fluid flowing through the synthesis channel portion, in which the synthesis channel portion includes a mixing channel portion that is a merging portion of the first channel and the second channel, a stagnation channel portion that communicates with a downstream side of the mixing channel portion and to which vibration is applied by the vibration applicator.

The present disclosure is related to an apparatus and system for synthesizing and administering particle-based therapeutics at the point-of-care. The apparatus includes a first chamber for receiving a first solution including lipids in a first solvent, a second chamber for receiving a second solution including nucleic acids in a second solvent, a mixing channel in communication with the first chamber and the second chamber, and a delivery system in communication with the to the mixing channel. The first solution in the first chamber and the second solution in the second chamber can be introduced into the mixing channel. The particle-based delivery system form in the mixing channel and the nucleic acids adhere to the particles. The formulated particle-based therapeutics are passed through a delivery system to a subject for administration.

A microfluidic chip is disclosed. According to an embodiment, a microfluidic chip including an inlet part in which a first inlet is provided into which a first fluid is injected; a middle part in which a first flow path is provided in which the first fluid can flow, wherein on the first flow path, a raw material storage part may be provided for storing a mixed raw material which can be mixed with the first fluid.