A microfluidic chip is disclosed. According to an embodiment, a microfluidic chip including an inlet part in which a first inlet is provided into which a first fluid is injected; a middle part in which a first flow path is provided in which the first fluid can flow, wherein on the first flow path, a raw material storage part may be provided for storing a mixed raw material which can be mixed with the first fluid.

This disclosure provides for devices, methods, and systems for generating a plurality of droplets within a collecting container at an extremely high rate (e.g., of at least 1 million droplets per minute, etc.), each of the plurality of droplets comprising an aqueous mixture for a digital analysis, wherein upon generation, the plurality of droplets is stabilized in position within a region of the collecting container. The inventions enable partitioning of samples for digital analyses at unprecedented rates, where readout of signals from targets within such partitions can still be achieved in accordance with various assays.

This disclosure provides for devices, methods, and systems for generating a plurality of droplets within a collecting container at an extremely high rate (e.g., of at least 1 million droplets per minute, etc.), each of the plurality of droplets comprising an aqueous mixture for a digital analysis, wherein upon generation, the plurality of droplets is stabilized in position within a region of the collecting container. The inventions enable partitioning of samples for digital analyses at unprecedented rates, where readout of signals from targets within such partitions can still be achieved in accordance with various assays.

This disclosure provides for devices, methods, and systems for generating a plurality of droplets within a collecting container at an extremely high rate (e.g., of at least 1 million droplets per minute, etc.), each of the plurality of droplets comprising an aqueous mixture for a digital analysis, wherein upon generation, the plurality of droplets is stabilized in position within a region of the collecting container. The inventions enable partitioning of samples for digital analyses at unprecedented rates, where readout of signals from targets within such partitions can still be achieved in accordance with various assays.

A system to produce a mixture of fluids includes a pressure source, a pressure regulator, at least a first container containing a first fluid and a second container containing a second fluid, and a microfluidic mixer. A control unit is configured to control the pressure level of the first fluid and the pressure level of the second fluid. The control unit is also configured to control an opening and closing of the first valve and of the second valve to inject the first fluid and second fluid successively into the microfluidic mixer, thereby generating a profile of fringes of the first fluid and of the second fluid within the common outlet channel.

The present invention relates to a sinuous microstructure mixing unit, and also relates to a microfluidic device having the unit, and the use of the unit or the device for preparing nanoparticles, such as lipid nanoparticles or self-assembled particles.

This disclosure provides for devices, methods, and systems for generating a plurality of droplets within a collecting container at an extremely high rate (e.g., of at least 1 million droplets per minute, etc.), each of the plurality of droplets comprising an aqueous mixture for a digital analysis, wherein upon generation, the plurality of droplets is stabilized in position within a region of the collecting container. The inventions enable partitioning of samples for digital analyses at unprecedented rates, where readout of signals from targets within such partitions can still be achieved in accordance with various assays.

A microfluidic device including a microfluidic circuit having a microfluidic inlet duct and a microfluidic outlet duct, and a microfluidic capsule, a hydraulic activation circuit having a hydraulic inlet duct and a hydraulic outlet duct, the microfluidic capsule having a chamber separated into two subspaces by a deformable membrane, the chamber having a first subspace into which open the microfluidic inlet duct and the microfluidic outlet duct, and a second subspace into which open the hydraulic inlet duct and the hydraulic outlet duct, a flow rate-control system connected to the hydraulic inlet duct, the flow rate-control system being regulated in order to inject or suck an activation liquid into the second subspace in order to displace the membrane inside the chamber.