The disclosure provides a new and improved oligonucleotide synthesizer.

A device for storing and circulating drinking water with vortex flow includes a base, a tank and an actuator housed in the base and configured to circulate the drinking water stored in the tank with vortex flow. The base includes an inlet port through which the drinking water is received and an outlet port through which the drinking water is dispensed. The tank is mounted on the base with a sealed engagement and is configured to store the drinking water. The tank has spherical or egg shape and includes an air inlet on an upper portion of the tank through which air can flow in and out of the tank.

A method includes, under control of control circuitry implementing a mixing protocol, aspirating reagents from multiple different reagent reservoirs into a cache channel. Designated amounts of the reagents are automatically aspirated from the corresponding reagent reservoirs by corresponding sippers based on the mixing protocol implemented by the control circuitry. The method also includes discharging the reagents from the cache channel into a mixing reservoir, and mixing the reagents within the mixing reservoir to form a reagent mixture.

A method for enhancing mixing and aeration of a liquid reaction medium in a toroidal bioreactor vessel includes dispensing a liquid reaction medium into an interior of a toroidal bioreactor vessel, the interior being bounded by an inner surface, a textured surface being arranged on at least a portion of the inner surface, the textured surface having a plurality of upstanding protuberances. The toroidal bioreactor vessel is rotated in an orbital motion such that there is a resonant frequency traveling wave of the fluid orbiting in one direction in the interior of the toroidal bioreactor vessel when a particular orbital speed is imparted to the toroidal bioreactor vessel.

An insert for a liquid-holding container may include a body comprising a wall, an open top end and a bottom end, and a generally tubular lumen extending from the open top end to the open bottom end. The insert may include a plurality of first openings formed in the wall, the first openings being situated between the top and bottom ends, wherein each of the plurality of first openings defines an area, and wherein the plurality of first openings have substantially the same dimensions. The insert may include one or more second openings formed in the wall, the one or more second openings being situated between the top and bottom ends, wherein each of the one or more second openings defines an area that is greater than the area defined by any of the first openings, wherein the one or more second openings have substantially the same dimensions.

A 5-liter bioreactor vessel enables a substantial increase in the volume of biological media that can be cultivated in the vessel compared to conventional designs. Moreover, when agitated at 1.5 the shaking frequency of a conventional 3-liter bioreactor (i.e., 90 rpm versus 60 rpm), the 5-liter vessel achieves a 19% increase in cell aeration without exceeding the maximum shear stress limit for cell viability. The 5-liter vessel optionally includes a plurality of internal baffles configured to disrupt the liquid vortex and reduce the maximum shear stress transferred to biomedia contained within the vessel.

A food preparation appliance has a base, a blade member rotatable in a direction of rotation, and a container with a closed bottom, an open top and a tubular sidewall together defining an interior space of the container. The top of the container is configured for releasable connection with the base, with the blade member disposed within the interior space of the container. The sidewall has a rib projecting inward of the interior space of the container and extending lengthwise along at least a portion of the sidewall from the top of the container toward the bottom of the container. The rib has, in the direction of rotation of the blade member, a peak defined as the shortest transverse dimension measured from the center of the container to the sidewall at a respective height along the portion of the sidewall having the rib, the rib being asymmetric in transverse cross-section.

A method includes, under control of control circuitry implementing a mixing protocol, aspirating reagents from multiple different reagent reservoirs into a cache channel. Designated amounts of the reagents are automatically aspirated from the corresponding reagent reservoirs by corresponding sippers based on the mixing protocol implemented by the control circuitry. The method also includes discharging the reagents from the cache channel into a mixing reservoir, and mixing the reagents within the mixing reservoir to form a reagent mixture.

A cylindrical or annular polymeric bioreactor is disclosed which provides enhanced mixing and aeration of the growth medium while simultaneously offering reduced mechanical shear force.

An impeller is usable in a variable diameter bioreactor having multiple vessel sections of successively increasing or decreasing volume. The impeller includes an impeller blade extending along an impeller blade axis between first and second axial ends and having opposed impeller blade faces, an impeller blade leading edge, and an impeller blade trailing edge. Each of the leading and trailing edges defines a helix or spiral between the axial ends of the impeller blade. In certain arrangements, the impeller blade is one of at least two impeller blades joined together along an impeller shaft extending axially along the impeller blade axis mentioned, and the helix or spiral has a pitch approximately equal to one half of a length between the first and second axial ends.