A method of dispensing a graded material includes generating droplets of a first working material, the droplets having a size in the range of 10 nanometers to 10 micrometers, adding the droplets of the first working material into a carrier fluid to create a first emulsion, wherein addition of the droplets of the first working material is controlled to create gradient in the emulsion, mixing the first emulsion to create a homogenous, graded mixture, and dispensing the homogenous, graded mixture onto a surface.

Disclosed herein is a cell culture device and its applications of creating gradient of chemicals or gradient of cells to mimic the in vivo physiological conditions of homeostasis.

A system for passive permeation of a biological material is disclosed. The system may include a main channel extending between an upper portion and a lower portion; a main reservoir connected to the upper portion of the main channel and in fluid communication with the main channel; a bottom reservoir connected to the lower portion of the main channel and in fluid communication with the main channel; at least one secondary channel disposed in at least one position between the upper portion and the lower portion of the main channel such that fluid communication is established in the at least one position between the at least one secondary channel and the main channel; and at least one secondary reservoir respectively connected to an upper portion of each of the at least one secondary channel and in fluid communication with the respective at least one secondary channel.

Described is a microfluidic serial dilution platform based well-plate using an oil-free immiscible phase driven by manual or electronic pipettors. The well-plate includes a plurality of fluidic traps, a plurality of hydrophilic capillary constriction channels and a plurality of bypass channels. Each of the plurality of bypass channels is associated with one of the plurality of fluidic traps, each of the plurality of hydrophilic capillary constriction channels is associated with one of the plurality of fluidic traps, and each of the plurality of fluidic traps is associated with one of the plurality of bypass channels and one of the plurality of hydrophilic capillary constriction channels. The well-plate further includes an inlet, an outlet, and a main channel with a plurality of portions that connects the inlet to the plurality of fluidic traps, associated hydrophilic capillary constriction channels and associated bypass channels, and the outlet.

A mixing system has a parallel droplet dispenser capable of making droplets of a first working material in the range of 10 nanometers to 10 micrometers, a pump to deliver fluid for the droplets of the first working material and to produce a first emulsion, a compact mixer having low inter-voxel mixing to receive the emulsion and produce a homogenous material, and a dispensing system. A method of dispensing a graded material includes generating droplets of a first working material, the droplets having a size in the range of 10 nanometers to 10 micrometers, adding the droplets of the first working material into a fluid to create a first emulsion, wherein addition of the droplets of the first working material is controlled to create gradient in the emulsion, mixing the first emulsion to create a homogenous, graded mixture, and dispensing the homogenous, graded mixture onto a surface.

Disclosed is a method and apparatus for manipulating fluids. The apparatus may include a microfluidic structure including inlet channels (1 and 2) and outlet channels (306, 307, 308, 309, 310, 311, 312, 313, and 314) oriented among bifurcated (5), trifurcated (6) and merging junctions (7 and 8). The apparatus splits and merges fluids flowing in the channels to produce successive dilutions of the fluids within the outlet channels. Multiple apparatus may be combined in serial, parallel, combined serial and parallel and/or stacked configurations. One or more apparatus may be used alone or to provide various devices or chambers with the diluted fluids.

A method and a system are described for mixing liquid chemicals at dynamically changing or static ratios during a given dispense, with extremely high uniformity and repeatability. A mixer includes multiple fluid supply lines including elongate bladders defining a linear flow path and being configured to laterally expand to collect a process fluid and laterally contract to deliver a selected volume of the process fluid to the mixer.

In one embodiment, a selective plane illumination microscopy system for capturing light emitted by an illuminated specimen, the system including a specimen support having a top surface configured to support a specimen holder and an opening configured to provide access to a bottom of the specimen holder, and a selective plane illumination microscopy optical system positioned beneath the specimen support, the optical system configured to illuminate the specimen with a sheet of excitation light and including an excitation objective, a detection objective, and an open-top, hollow imaging element that is configured to contain a liquid, wherein the imaging element is positioned within the opening of the specimen support and optical axes of the objectives are aligned with the imaging element such that the axes pass through the imaging element and intersect at a position near the top surface of the specimen support.

Provided herein are compositions, systems, and methods for high throughput screening. In particular, provided herein are microfluidic devices for high throughput analysis of multiplex chemical (e.g., drug interactions) across a wide range of concentrations.

A method of dispensing a graded material includes generating droplets of a first working material, the droplets having a size in the range of 10 nanometers to 10 micrometers, adding the droplets of the first working material into a carrier fluid to create a first emulsion, wherein addition of the droplets of the first working material is controlled to create gradient in the emulsion, mixing the first emulsion to create a homogenous, graded mixture, and dispensing the homogenous, graded mixture onto a surface.