A method for purifying a non-aqueous solvent includes: pretreating by passing a non-aqueous solvent for dehydration treatment through a packed bed of an ion exchange resin that is not yet subjected to dehydration treatment, to remove water in the ion exchange resin; and purifying by passing a non-aqueous solvent to be purified through the packed bed of the ion exchange resin from which water is removed during the pretreating, to purify the non-aqueous solvent to be purified, in which the harmonic-mean particle size of the ion exchange resin is 0.20 to 0.50 mm. According to the present application, a method for purifying a non-aqueous solvent can be provided, the method includes pretreating by removing water in a water-containing ion exchange resin by passing therethrough a non-aqueous solvent for dehydration treatment, in which the amount of the non-aqueous solvent for dehydration treatment used during the pretreating is small.

A method for purifying a non-aqueous solvent includes: pretreating by passing a non-aqueous solvent for dehydration treatment through a packed bed of an ion exchange resin that is not yet subjected to dehydration treatment, to remove water in the ion exchange resin; and purifying by passing a non-aqueous solvent to be purified through the packed bed of the ion exchange resin from which water is removed during the pretreating, to purify the non-aqueous solvent to be purified, in which the harmonic-mean particle size of the ion exchange resin is 0.20 to 0.50 mm. According to the present application, a method for purifying a non-aqueous solvent can be provided, the method includes pretreating by removing water in a water-containing ion exchange resin by passing therethrough a non-aqueous solvent for dehydration treatment, in which the amount of the non-aqueous solvent for dehydration treatment used during the pretreating is small.

A purification method for separating and purifying an organic solvent from a liquid mixture of an organic solvent and water, the organic solvent having a boiling point of more than 100° C. at 1 atm, includes the steps of: passing the liquid mixture through a first ion exchange device; supplying the liquid mixture discharged from the first ion exchange device to a pervaporation device to selectively separate water component; supplying the organic solvent recovered from the concentration side of the pervaporation device to an evaporator to obtain a purified organic solvent; and passing, through the second ion exchange device, a portion of liquid containing the organic solvent and flowing at a first position subsequent to the first ion exchange device. The liquid discharged from the second ion exchange device is returned to a second position which is at a preceding stage of the pervaporation device.

A method of producing treated ion exchange resin material includes exposing an enclosed vessel containing ion exchange resin and a pre-treatment solution to high energy radiation. The treated ion exchange resin material has reduced organic impurities or total organic carbon (TOC).

A method of producing treated ion exchange resin material includes exposing an enclosed vessel containing ion exchange resin and a pre-treatment solution to high energy radiation. The treated ion exchange resin material has reduced organic impurities or total organic carbon (TOC).

A method for purification of a sulfatase using metal chelating chromatography without using tags such as His-tag, etc. is disclosed. An embodiment provides a method for purifying a sulfatase including the steps of: (a) providing a sulfatase-containing solution comprising one or a plurality of impurities; (b) performing a first chromatographic separation of the sulfatase-containing solution using a metal affinity chromatography resin; (c) performing a second chromatographic separation using a cation exchange chromatography resin; and (d) performing a final chromatographic separation using an anion exchange chromatography resin, wherein the impurities are removed thereby.

The present disclosure relates to methods, compositions and kits useful for the enhanced pH gradient cation exchange chromatography of a variety of analytes. In various aspects, the present disclosure pertains to chromatographic elution buffer solutions that comprise a first buffer salt, a second buffer salt, a third buffer salt, and fourth buffer salt. The first buffer salt may be, for example, a diprotic acid buffer salt, the second buffer salt may be, for example, a divalent buffer salt with two amine groups, the third buffer salt may be, for example, a monovalent buffer salt comprising a single amine group, and the fourth buffer salt may be, for example, a zwitterionic buffer salt. Moreover, the buffer solution has a pH ranging from 3 to 11.

The present disclosure relates to methods, compositions and kits useful for the enhanced pH gradient cation exchange chromatography of a variety of analytes. In various aspects, the present disclosure pertains to chromatographic elution buffer solutions that comprise a first buffer salt, a second buffer salt, a third buffer salt, and fourth buffer salt. The first buffer salt may be, for example, a diprotic acid buffer salt, the second buffer salt may be, for example, a divalent buffer salt with two amine groups, the third buffer salt may be, for example, a monovalent buffer salt comprising a single amine group, and the fourth buffer salt may be, for example, a zwitterionic buffer salt. Moreover, the buffer solution has a pH ranging from 3 to 11.

The present invention relates to the separation and isolation of neutral human milk oligosaccharides (HMOs) from the reaction mixture in which they are produced.

The present invention relates to the separation and isolation of neutral human milk oligosaccharides (HMOs) from the reaction mixture in which they are produced.