Bare porous polymer monoliths, fluidic chips, methods of incorporating bare porous polymer monoliths into fluidic chips, and methods for functionalizing bare porous polymer monoliths are described. Bare porous polymer monoliths may be fabricated ex situ in a mold. The bare porous polymer monoliths may also be functionalized ex situ. Incorporating the bare preformed porous polymer monoliths into the fluidic chips may include inserting the monoliths into channels of channel substrates of the fluidic chips. Incorporating the bare preformed porous polymer monoliths into the fluidic chips may include bonding a capping layer to the channel substrate. The bare porous polymer monoliths may be mechanically anchored to channel walls and to the capping layer. The bare porous polymer monoliths may be functionalized by ex situ immobilization of capture probes on the monoliths. The monoliths may be functionalized by direct attachment of chitosan.

The present disclosure is directed to mixed mode chromatography media comprising a ligand directly attached to a solid support. In some aspects, the ligand has a chemical formula of ##STR00001##
The mixed mode chromatography media is useful for binding and purifying proteins from a solution.

The present invention provides porous materials for use in solid phase extractions and chromatography. In particular, the materials exhibit superior properties in the SPE analysis of biological materials. In certain aspects, the materials feature at least one hydrophobic component, at least one hydrophilic component and a average pore diameter of about 100 to about 1000 . In certain embodiments the materials also exhibit a nitrogen content from about 1% N to about 20% N. In certain embodiments, the materials feature at least one hydrophobic component, at least one hydrophilic component wherein more than 10% of the BJH surface area of the porous material is contributed by pores that have a diameter greater than or equal to 200 .

The present invention provides novel chromatographic materials, e.g., for chromatographic separations, processes for their preparation and separations devices containing the chromatographic materials. The preparation of the inorganic/organic hybrid materials of the invention wherein a surrounding material is condensed on a superficially porous hybrid core material will allow for families of different hybrid packing materials to be prepared from a single core hybrid material. Differences in hydrophobicity, ion-exchange capacity, chemical stability, surface charge or silanol activity of the surrounding material may be used for unique chromatographic separations of small molecules, carbohydrates, antibodies, whole proteins, peptides, and/or DNA.

An object of the present invention is to provide a novel method of recovering a metal and/or a metal ion from a liquid to be treated, by use of a monolith adsorbent, and a novel method of regenerating an adsorbent used in the method of recovering a metal and/or a metal ion from a liquid to be treated, and, in order to achieve the object, the present invention provides a method of recovering a metal and/or a metal ion, the method including the following steps of: (1) preparing for a solution containing a metal and/or a metal ion; (2) preparing for an adsorbent having a co-continuous structure formed by: a ceramic skeleton including mesopores; and macropores, wherein a surface of the ceramic skeleton is modified by a metal- and/or metal ion-adsorbable functional group, a most frequent pore diameter of the macropores before modification by the functional group is 0.20 ?m or more and 4.0 ?m or less, and a most frequent pore diameter of the mesopores before modification by the functional group is 2.0 nm or more and 50 nm or less; (3) contacting the solution and the adsorbent; (4) contacting the adsorbent subjected to step (3) and an acidic solution; and (5) recovering the metal and/or the metal ion from the acidic solution subjected to step (4).

An object of the present invention is to provide a monolith columnar body capable of satisfying both adsorption performance and durability, a monolith adsorbent using the monolith columnar body, and a method of removing a metal and/or a metal ion from a liquid to be treated, with the monolith adsorbent, and, in order to achieve the object, the present invention provides a columnar body (1) having a co-continuous structure formed by: a ceramic skeleton (2) including mesopores (4); and macropores (3), wherein an average diameter of the columnar body (1) is 1.5 mm or more and 20 mm or less, wherein a most frequent pore diameter of the macropores (3) is 0.20 ?m or more and 4.0 ?m or less, and wherein a most frequent pore diameter of the mesopores (4) is 2.0 nm or more and 50 nm or less.

Thin layer chromatography (TEC) devices for the analysis of pico-scale samples, methods for using the devices, and methods for fabricating the devices.

The present invention describes the process of preparing ceramic materials for absorption of acidic gases, mainly carbon dioxide, in exhaust systems and/or present indoors. Ceramic materials are formed by a mixture of alkali carbonate with alkaline earth metal oxide/hydroxide associated with a binding component, but non-limiting. The alkali carbonate comprises sodium, potassium carbonate, or a mixture of both. The alkaline earth metal oxide/hydroxide may be formed from magnesium oxide or magnesium hydroxide as well as calcium oxide and/or calcium hydroxide.

Provided herein are methods of liquid column chromatography in which preparative chromatography is performed in-line with analytical chromatography. In particular aspects a monolithic preparative column is used to purify an analyte of interest from a mixture of other substances by applying the mixture to the column, reversing the flow through the column to elute the analyte, which is applied to an analytical column provided in-line with the preparative column. In other aspects, a single monolithic column is used to perform both the preparative chromatography and analytical chromatography steps in succession. In another aspect, a chromatography system is provided to perform preparative and analytical chromatography using a single monolithic column.

The present invention provides novel chromatographic materials. e.g., for chromatographic separations, processes for their preparation and separations devices containing the chromatographic materials. The chromatographic materials of the invention have controlled porosity and comprise a chromatographic core material and one or more layers of chromatographic surface material which each independently provide an average pore diameter, an average pore volume, or a specific surface area such that the combined layers form a chromatographic material having a predetermined or desired pattern of porosity from the core material to the outermost surface. The materials are useful for HPLC separations, normal-phase selarations, reversed-phase separations, chiral separations, HILIC separations, SFC separations, affinity separations, perfusive separations, partially perfusive separations, and SEC separations.