A fluidic system that includes a reagent manifold comprising a plurality of channels configured for fluid communication between a reagent cartridge and an inlet of a flow cell; a plurality of reagent sippers extending downward from ports in the manifold, each of the reagent sippers configured to be placed into a reagent reservoir in a reagent cartridge so that liquid reagent can be drawn from the reagent reservoir into the sipper; at least one valve configured to mediate fluid communication between the reservoirs and the inlet of the flow cell. The reagent manifold can also include cache reservoirs for reagent re-use.

A multi-actuated micro-pipette controller is actuated in at least three different ways. First, the multi-actuated micro-pipette controller may be employed similar to a striper for denudation of cumulus cells and transfer of oocytes and embryos by depressing an actuator assembly operably coupled to a ballonet. Second, the multi-actuated micro-pipette controller may be actuated similar to a SWEMED™ denudation pipette by pressing directly on the ballonet while it is seated in a primary housing and a secondary housing, without employing an actuator assembly of the primary housing. Third, the multi-actuated micro-pipette controller may be actuated by removing the ballonet and employing it similar to a PASTEUR™ pipette.

A washing device comprises a fluid dispenser that operably directs a cleaning fluid to contact one or more objects to be cleaned, the one or more objects to be cleaned being contained by the washing device, a dispensing line directing the cleaning fluid to the fluid dispenser, a compressed air source, an air valve selectively controlling flow of air from the compressed air source into the dispensing line, a cleaning fluid source, a pump selectively pumping the cleaning fluid from the cleaning fluid source into the dispensing line, and a controller. The controller alternatingly activates (a) the pump to pump the cleaning fluid from the cleaning fluid source into the dispensing line and (b) the air valve to force the cleaning fluid from the dispensing line via the fluid dispenser.

Disclosed is a reaction method that includes a reaction process using a pipette tip to allow substances to cause a reaction, the method including: providing a heater for heating the pipette tip in accordance with a preset temperature; attaching the pipette tip to a pipette nozzle and heating the pipette tip by a first preset temperature; when a period of heating by the first preset temperature exceeds a preset period, switching an output of the heater from the first preset temperature to a lower second preset temperature; detecting a distal end position of the pipette tip at or after the switching; and executing the reaction process while controlling the distal end position of the pipette tip with reference to the detected distal end position, in which the temperature of the pipette tip is kept by the second preset temperature at least until operation of the pipette tip.

Disclosed in the present application is a sample adding needle for preparing microdroplets, comprising a liquid storage portion and a liquid discharge portion, which are integrally injection molded and penetrate one another; the liquid storage portion is a truncated cone the radial dimension of which gradually decreases in the direction facing the liquid discharge portion, and the liquid discharge portion is a truncated cone the radial dimension of which gradually decreases in the direction away from the liquid storage portion; the taper of the liquid storage portion is C1, the taper of the liquid discharge portion is C2, and C1≤C2; the wall thickness of the liquid storage portion is D1, the wall thickness of the liquid discharge portion is D2, and D1>D2. For the sample adding needle for preparing microdroplets as described in the present application, when preparing microdroplets by using the sample adding needle, the sample adding needle performs periodic reciprocating motion at varying speeds in an oily liquid such that a sample solution is subject to periodic shear force from the oily liquid at a liquid discharge opening, thereby enabling the sample solution within the sample adding needle to enter the oily liquid, thus achieving the production of microdroplets having uniform size and controllable volume.

A pipette tip made of plastic includes a tubular body having an inner circumference and an outer circumference. The tubular body includes a bottom end defining a bottom opening structured to enable passage of a liquid and a top end defining a top opening that clamps onto a mount of a pipetting device. A seat region for the mount is positioned proximate to the top opening and on the inner circumference of the tubular body. A plurality of flattenings extend in an axial direction on the outer circumference proximate to the top opening. The tubular body defines a contour of an arc polygon in a cross-section through the flattenings on the outer circumference.

A pipette tip device for use in dispersive SPE. The device includes a pipette tip having a lower barrier, loose sorbent that is freely moveable during the extraction process, and a baffle system that is shaped to disrupt the flow of liquid sample that is aspirated into the pipette tip. The baffle system includes an insert that may be separate from or monolithic with the interior of the pipette tip.

Reagent carrier unit comprising a coupling section for releasable coupling with a pipetting arm coupling section of a pipetting means of a laboratory robot (1) or with a pipetting tip (19) of a pipetting means of a laboratory robot (1).

The present invention is directed towards an apparatus and methods for a precise, fast and easy to use manipulation of beads. This method is particularly useful to carry out separation between the beads and the remaining supernants present in the fluid, maximizing the collection and purification efficiencies in tips for liquid handling.

A pipette tip having a first end arranged to be fitted to a pipette body (3) of a pipette (1) and having a second end with an opening (15) arranged to be inserted in a sample which is at least in part intended to be drawn up by the pipette, wherein the opening (15) has a sharp profile such that it can be used to cut through a cover (119) covering an enclosure containing the sample.