There is provided a nucleic acid extraction method using a cartridge comprising: (a) a driving part of a nucleic acid extraction device is connected to a control rod module disposed in an inner space of the upper body of a piston and a rotation control module coupled to the lower body of the piston; (b) driving the rotation control module and the control rod module, sequentially sucking sample and reagents from the plurality of chambers separated from each other into an interior space, and discharging the mixture of the interior space into the chamber of the cartridge; and (c) driving the rotation control module and the control rod module to suck the reagent inside the master mix bead chamber of the cartridge into the interior space of the piston upper body and then discharge the mixed reagent to a nucleic acid amplification module.

An apparatus includes a fluid reservoir and a laminate fluidic circuit positioned above the fluid reservoir. The laminate fluidic circuit includes two or more layers laminated together to define a substantially planar substrate and one or more channels defined within the substrate. The laminate fluidic circuit includes a flexible conduit defined by a portion of the substrate encompassing an extent of at least one of the channels that is partially separated or separable from the remainder of the substrate. The flexible conduit is deflectable with respect to the planar substrate toward the fluid reservoir such that the flexible conduit fluidly connects the at least one channel to the fluid reservoir.

Assay system including assay apparatus and handheld single use assay devices for ElectroMagnetic Radiation (EMR) examination of a specimen. The assay devices include a housing, a specimen slide for elevation from an initial lowermost specimen introduction position to a final uppermost specimen examination position and a built-in liquid reagent dispensing arrangement for dispensing liquid reagent on the specimen slide. The assay apparatus automatically actuates an assay device and includes an imaging arrangement for obtaining specimen images.

Instrument for performing a diagnostic test on a fluidic cartridge A cartridge reader is for carrying out a diagnostic test on a sample contained in a fluidic cartridge inserted into the reader. The fluidic cartridge comprises a fluidic layer comprising at least one sample processing region, at least one collapsible blister containing a liquid reagent, a pneumatic interface, an electrical interface and at least one mechanical valve. The reader comprises a housing; an upper clamp occupying a fixed position relative to the reader, and a lower clamp, movable relative to the first clamp, wherein the upper clamp and the lower clamp define a cartridge receiving region therebetween. The reader comprises a thermal module comprised in the lower clamp, wherein the thermal module comprises at least one thermal stack for heating the at least one sample processing region of the cartridge inserted into the reader. The reader comprises at least one mechanical actuator for actuating the mechanical valve comprised in the cartridge inserted into the reader.

An apparatus for preparing a reagent solution includes an enclosure and a container disposed within the enclosure. The container defines an internal cavity having a compressible volume and defines a passage providing fluidic communication between the internal cavity and the exterior of the container. Optionally, a compressible member is disposed within the internal cavity. A reagent is disposed within the internal cavity.

A system and method for isolating and analyzing single cells, wherein the system includes: an array of wells defined at a substrate, each well including an open surface and a well cavity configured to capture cells in one of a single-cell format and single-cluster format, and a fluid delivery module including a fluid reservoir superior to the array of wells through which fluid flow is controlled along a fluid path in a direction parallel to the broad face of the substrate; and wherein the method includes: capturing a population of non-cell particles into the array of wells in single-particle format; releasing, from the non-cell particles, a set of probes into the array of wells; capturing a population of cells into the array of wells in single-cell format; releasing biomolecules from each captured cell into the array of wells; and generating a set of genetic complexes comprising the biomolecules associated with a single captured cell and a subset of probes within individual wells of the array of wells.

The dispensing unit includes a disc, a cartridge, and a dispensing holder. The disc has a disc shape and includes a track region provided with recesses and projections alternately arranged in a radial direction. The cartridge includes a penetration hole, and a well is formed by the penetration hole and the track region in a state in which the cartridge is attached to the disc. The dispensing holder includes a holding part to be inserted into the penetration hole, and a guide hole penetrating the holding part. The guide hole has a truncated cone shape in which a first opening diameter on the holding part side is smaller than a second opening diameter on a side opposite to the holding part, and a center line of the guide hole is located on a line passing through the center of the disc and the center of the well.

A unitary, molded fluid reservoir body to which a fluid ejection head substrate is attached. The unitary, molded fluid reservoir body includes one or more discrete fluid chambers therein. Each of the one or more fluid chambers have an open top, side walls, and sloped bottom walls attached to the side walls, wherein each of the one or more fluid chambers terminates in a fluid supply via, and wherein the sloped bottom walls have an angle ranging from about 6 to about 12 degrees relative to a plane orthogonal to the sidewalls. An ejection head support face is disposed opposite the open top for attachment of a single fluid ejection device to the ejection head support face for ejecting fluid provided from the one or more chambers through the one or more fluid supply vias.

A reagent sleeve system such as can be used with various analyzers using chemical phenomena, analyzers and methods of using the reagent sleeve system and analyzer are described. The reagent sleeve system can include a reagent sleeve; and a plurality of removable reagent reservoirs located within the reagent sleeve, wherein each of the plurality of removable reagent reservoirs comprises a respective peripheral wall, and first and second end walls, whereby each of the plurality of removable reagent reservoirs contains a reagent located therein, and the first end wall is pierceable by a reagent extractor, and the reagent sleeve comprises a peripheral wall that encompasses the plurality of removable reagent reservoirs and holds the plurality of reagent reservoirs for sequential use.