In a flow path device, a first groove, a second groove and a third groove are provided. The first groove is connected to and continuous with a first hole. The second groove is connected to and continuous with the first groove. The third groove is connected to and continuous with the second groove. The third groove is connected to the second groove at a position on the second groove spaced from the first groove. The first groove extends toward a position opposite to the second groove with respect to the first hole. The second groove and the third groove define a first minor angle adjacent to the first hole and define a second minor angle opposite to the first hole. The first minor angle is larger than the second minor angle.

Provided herein are systems and methods for a point of need testing system. In some embodiments, the point of need testing system includes a first collector and a sample amplifier. The first collector is configured to collect a first biofluid sample. The sample amplifier includes an inlet dimensioned to receive the first biofluid sample. The sample amplifier further includes a filter, a heater, and a second collector. In some embodiments, a reader can be used with the sample amplifier to read a second biofluid produced by the sample amplifier.

A detection cassette for detecting a sample is provided. The sample includes a first component and a second component. The detection cassette includes a sample injection hole, first and second separation tanks communicated with the sample injection hole, and first and second detection tanks communicated with the first and second separation tanks. A part of the sample enters the first separation tank from the sample injection hole and separated into the first component and the second component in the first separation tank. Another part of the sample enters the second separation tank from the sample injection hole and separated into the first component and the second component in the second separation tank. The first component separated in the first separation tank flows to the first detection tank and the second component separated in the second separation tank flows to the second detection tank for detections. A detection system is provided.

This work provides a method and device for performing quantitative and sensitive multiplex nucleic acid detection at the point-of-care using magnetoresistive (MR) detection. Temperature calibration of the MR sensor elements is performed per-element, rather than assuming the same calibration parameters apply to each element of the MR sensor array. It can include a digitally controlled fluidic system to allow automated wash and reagent injection, an on-chip temperature management system to achieve on-chip polymerase chain reactions (PCR), and a portable magnetoresistive sensor platform. This approach requires minimal user involvement beyond adding the sample and simple top-level control, making it highly desirable for point-of-care applications.

Detection devices, systems, and methods for detecting an analyte in a fluid sample are provided. The detection devices, systems, and methods can include colorimetric detection to rapidly determine the presence and/or quantity of the analyte obtained from a surface that is contaminated or suspected of being contaminated with the analyte. In one aspect, the detection device includes a sample reservoir in fluid communication with a fluid flow path. The fluid flow path includes a control well downstream of the sample reservoir, a valve assembly downstream of the control well, a reagent well downstream of the valve assembly, and a test well downstream of the reagent well. In one aspect, the reagent well includes a dried reducing agent configured to generate a gas in the presence of a detection dye and the analyte in the fluid sample.

The present invention provides methods and devices for simple, low power, automated processing of biological samples through multiple sample preparation and assay steps. The methods and devices described facilitate the point-of-care implementation of complex diagnostic assays in equipment-free, non-laboratory settings.

A liquid handling device having an axis of rotation about which the device can be rotated to drive liquid flow. The device includes a vented upstream chamber having an outlet port and an unvented chamber including an inlet port to receive liquid from the outlet port of the upstream chamber and an outlet port radially outward the inlet port. The device further includes a vented downstream chamber having an inlet port to receive liquid from the outlet port of the unvented chamber. A downstream conduit connects the outlet port of the unvented chamber to the inlet port of the downstream chamber and includes a bend radially inward of the outlet port of the unvented chamber. An upstream conduit connects the outlet port of the upstream chamber to the inlet port of the unvented chamber.

A device for the processing of a sample comprises a location apparatus, a processing chamber for receiving the sample and a plurality of reagent chambers. The reagent chambers have openings defined in the location apparatus. The processing chamber is movable relative to the reagent chambers to enable sequential communication with each reagent chamber in turn.

The present disclosure provides a cartridge for use in nucleic acid extraction and a nucleic acid extraction method which can simplify a nucleic acid extraction process and can be applicable to a point-of-care testing (POCT). The cartridge includes a chamber module, an air valve module, and a liquid valve module. The chamber module includes a plurality of chambers for extracting nucleic acids from a sample including a pretreatment chamber in which the sample is crushed and homogenization, cell disruption, and purification are performed. The air valve module is installed on the chamber module to control a pressure required to move a fluid between the plurality of chambers. The liquid valve module is installed below the chamber module to move the fluid between the plurality of chambers.

A substrate for sample analysis including: a substrate including a rotation axis; a first chamber, which includes a first space which retains the liquid; a second chamber, which includes a second space which retains the liquid discharged from the first chamber; and a first flow passage, which includes a path connecting the first chamber and the second chamber in which the first flow passage has a first opening and a second opening, the first opening and the second opening are connected to the first chamber and the second chamber, respectively, and the first opening is positioned on a side closer to the rotation axis than the second opening, in which the first space includes a first region, which includes a portion extending from the first opening and in which the first space of the first chamber has a capacity larger than a capacity of the first flow passage.