Examples herein involve amplification and detection of nucleic acids using a microfluidic reaction chamber. An example apparatus includes a reaction-chamber circuit to process a reagent and a biologic sample for amplification of nucleic acids. The apparatus further includes a plurality of capillaries to pass the reagent and the biologic sample through the microfluidic reaction chamber. A valve control system may selectively control each of a plurality of valves to cause the reagent and the biologic sample to selectively move through the microfluidic reaction chamber for the amplification of the nucleic acids according to a particular timing sequence. In various examples, a trapping region disposed in the microfluidic reaction chamber secures the nucleic acids in the microfluidic reaction chamber for amplification using the reaction-chamber circuit

A fluidic system that includes a reagent manifold comprising a plurality of channels configured for fluid communication between a reagent cartridge and an inlet of a flow cell; a plurality of reagent sippers extending downward from ports in the manifold, each of the reagent sippers configured to be placed into a reagent reservoir in a reagent cartridge so that liquid reagent can be drawn from the reagent reservoir into the sipper; at least one valve configured to mediate fluid communication between the reservoirs and the inlet of the flow cell. The reagent manifold can also include cache reservoirs for reagent re-use.

A diagnostic device 10 for screening for a target analyte in a sample is provided. The diagnostic device 10 comprises a substrate 12 and a hydrophobic material 20 disposed on the substrate. The hydrophobic material 20 is selected to be converted from the hydrophobic material 20 to a hydrophilic material 22 upon contact with a conversion component within or derived from a sample introduced to the device 10.

The disclosed apparatus, systems and methods relate to a modular microfluidic device, the component comprising a channel comprising an apex opening, wherein the apex opening that is at least partially surrounded by a collar configured to pin a liquid within the channel. The modular microfluidic device may also have a structural tip in or near the apex opening which is configured to allow for the flow of the liquid into the channel from a second component for a modular microfluidic device when the component is engaged with the second component.

A microfluidic apparatus mounted on a rotation driving unit for inducing a fluid to flow due to a centrifugal force includes: a target chamber that houses a first fluid; a first chamber that houses a second fluid and is disposed closer to a rotation center of the microfluidic apparatus in a radius direction than the target chamber, the first chamber being connected to the target chamber by a first channel; a first valve that prevents a flow of the second fluid through the first channel; and a second chamber disposed closer to the rotation center in the radius direction than the target chamber and connected to the target chamber by a second channel, wherein the first fluid is transported to the second chamber by supplying the second fluid to the target chamber by the centrifugal force.

The present invention provides a microfluidic device which comprises a drive module and a microfluidic platform. The drive module further comprises a rotary unit and a vibration unit for driving the microfluidic platform, and the microfluidic platform further comprises multiple microfluidic elements for performing tests. The present invention also provides a method for operating a microfluidic device. The method comprises steps using the rotary unit and steps using the vibration unit to distribute sample in a microfluidic structure.

A biological substance extraction device includes an adsorption container that includes a first flow channel, and seal-tightly holds an adsorbent and a fluid within the first flow channel, and a washing container that includes a second flow channel, and seal-tightly holds a washing liquid and a fluid within the second flow channel, the adsorption container and the washing container being joined to form a flow channel through which a biological substance is moved. The first flow channel and the second flow channel communicate with each other in a state in which an insertion section is inserted into a reception section. The insertion section includes a guide member that extends from the first flow channel to the second flow channel. The guide member forms part of the flow channel between a first inner wall of the first flow channel and a second inner wall of the second flow channel.

A sample loading cartridge (1) for a microfluidic device comprises a cartridge body (10) with a sample reservoir (20) configured to house a volume of a liquid sample (3) and a sample port (30) in connection with the sample reservoir (20). The cartridge (1) also comprises an output channel (40) extending from the sample reservoir (20) and a feedback channel (50) connected to the sample reservoir (20) and to the sample port (30). The cartridge body (10) comprises a detection portion (60) aligned with the feedback channel (50) to enable detection of any sample (3) in the feedback channel (50). The flow resistance of the feedback channel (50) is lower than the flow resistance of the output channel (40) to cause liquid sample (3) received in the sample port (30) to enter the feedback channel (50) with substantially no liquid sample (3) entering the output channel (40).

Assay cartridges are described that have purification, reaction, and detection zones and other fluidic components which can include sample chambers, waste chambers, conduits, vents, reagent chambers, reconstitution chambers and the like. The assay cartridges are used to conduct multiplexed nucleic acid measurements. Also described are kits including such cartridges, methods of using the same, and a reader configured to analyze an assay conducted using an assay cartridge.

A storage assembly for two substances to be mixed prior to use, includes a closable dropper tip (3). A first storage chamber (7) is formed by the closable dropper tip (3) and a first container part (2, 4), the first container part (2, 4) being provided with at least one aperture (43). A second storage chamber (8) is formed by a second container part (5) which includes an open end part (52, 53) that closes off the at least one aperture (43) in a first operational position. The first and second container parts (2, 4; 5, 6) are moveable with respect to each other, and are in fluid communication through the at least one aperture (43) in a second operational position wherein the total flow surface area of the at least one aperture (43) is at least equal to a cross sectional area of the first container part.