A disease screening device (100) comprising a substrate (101) and a sonication chamber (102) formed on the substrate (101). The sonication chamber (102) is provided with an ultrasonic transducer (105) which generates ultrasonic waves to lyse cells in a sample fluid within the sonication chamber (102). The device (100) comprises a reagent chamber (111) formed on the substrate (101) for receiving a liquid PCR reagent. The device (100) comprises a controller (23) which controls the ultrasonic transducer (105) and a heating arrangement (128) which is provided on the substrate (101). The device (100) further comprises a detection apparatus which detects the presence of an infectious disease, such as COVID-19 disease.

Bare porous polymer monoliths, fluidic chips, methods of incorporating bare porous polymer monoliths into fluidic chips, and methods for functionalizing bare porous polymer monoliths are described. Bare porous polymer monoliths may be fabricated ex situ in a mold. The bare porous polymer monoliths may also be functionalized ex situ. Incorporating the bare preformed porous polymer monoliths into the fluidic chips may include inserting the monoliths into channels of channel substrates of the fluidic chips. Incorporating the bare preformed porous polymer monoliths into the fluidic chips may include bonding a capping layer to the channel substrate. The bare porous polymer monoliths may be mechanically anchored to channel walls and to the capping layer. The bare porous polymer monoliths may be functionalized by ex situ immobilization of capture probes on the monoliths. The monoliths may be functionalized by direct attachment of chitosan.

A disease screening device (100) comprising a substrate (101) and a sonication chamber (102) formed on the substrate (101). The sonication chamber (102) is provided with an ultrasonic transducer (105) which generates ultrasonic waves to lyse cells in a sample fluid within the sonication chamber (102). The device (100) comprises a reagent chamber (111) formed on the substrate (101) for receiving a liquid PCR reagent. The device (100) comprises a controller (23) which controls the ultrasonic transducer (105) and a heating arrangement (128) which is provided on the substrate (101). The device (100) further comprises a detection apparatus which detects the presence of an infectious disease, such as COVID-19 disease.

A solid phase microextraction device is disclosed, including a substrate having at least one planar surface, a sorbent layer disposed on at least a portion of the at least one planar surface, a tapering tip extending from the substrate, a receptacle mount configured for removable attachment to an emplacement of a receiving device, and a clocking feature configured for fixing a radial orientation of the at least one planar surface with respect to the receiving device. A solid phase microextraction device repository is disclosed including a wall surrounding a chamber, a plurality of orifices disposed in the wall configured to receive and retain the device, and a plurality of clocking feature interfaces disposed in the wall. A solid phase microextraction device manipulator is disclosed, including a manipulator shaft, an emplacement configured to removably engage a receptacle mount, an electrically conductive contact disposed at the emplacement, an ejector, and a clocking feature interface.

Devices and methods are provided for electrically lysing cells and releasing macromolecules from the cells. A microfluidic device is provided that includes a planar channel having a thickness on a submillimeter scale, and including electrodes on its upper and lower inner surfaces. After filling the channel with a liquid, such that the channel contains cells within the liquid, a series of voltage pulses of alternating polarity are applied between the channel electrodes, where the amplitude of the voltage pulses and a pulse width of the voltage pulses are effective for causing irreversible electroporation of the cells. The channel is configured to possess thermal properties such that the application of the voltage produces a rapid temperature rise as a result of Joule heating for releasing the macromolecules from the electroplated cells. The channel may also include an internal filter for capturing and concentrating the cells prior to electrical processing.

According to a first aspect, the present invention is embodied as a method of processing a filtered liquid with a microfluidic device. The method includes positioning a porous filtering medium with respect to the microfluidic device, so as to allow a flow path between the filtering medium and a channel of the microfluidic device. The method further includes introducing a liquid in the porous filtering medium for the liquid to advance along the filtering medium and be filtered by the medium. The method further includes applying compression to the filtering medium to extract a given volume of the filtered liquid from the filtering medium, where the extracted liquid volume reaches said channel via the flow path. The method further includes processing the extracted volume with the microfluidic device.

The present invention provides a disposable pipette tip for dispersive solid phase extraction (SPE) that allows for rapid, automatable purification of large biomolecules, such as nucleic acids, proteins and polypeptides without the need for additional tools such as centrifuges, magnetic plates or vacuum manifolds. The pipette tip is designed for optimal biomolecule isolation while maintaining sample integrity. Optimized methods of using the dispersive pipette extraction system for isolation of large biomolecules are also provided.

A laboratory system and a method for separating interfering substances contained in test samples is presented. The laboratory system comprises separation vessels comprising solid surfaces and capturing molecules which are immobilized on the solid surfaces. The capturing molecules of the separation vessels are configured to bind interfering substances of laboratory tests of different analytical methods.

Methods and systems and related compositions for separating through a solid matrix a mixture comprising a nucleic acid together with a target compound having a water solubility equal to or greater than 0.01 mg per 100 mL, which can be used for managing fluid flow, biochemical reactions and purification of the nucleic acid or other target analytes.

A disposable cartridge for use in a diagnostic assay system including a cartridge body defining a barrel port. A rotor is rotationally mounted to the cartridge body and comprises a plurality of assay chambers and a plurality of rotor ports each fluidly coupled to at least one of the plurality of assay chambers. A syringe is coupled to the cartridge body and operative to inject and withdraw assay fluids through the barrel port. The syringe includes a barrel defining an internal environment, a plunger positioned in the barrel and structured to move within a working area, and a disposable shaft structured to detachably mate at a first end with the plunger. The disposable shaft is isolated from the internal environment of the barrel.