The present invention is directed to sample test cards having an increased sample well capacity for analyzing biological or other test samples. In one embodiment, the sample test cards of the present invention comprises a fluid channel network disposed in both the first surface and the second surface and connecting the fluid intake port to the sample wells, the fluid channel network comprising at least one distribution channels, a plurality of fill channels operatively connected to the at least one distribution channel, a plurality of through-channels operatively connected to one or more of the fill channels and a plurality of horizontally orientated fill ports operatively connecting the fill channels to the sample wells.

A device for dispensing one or more microdroplets is provided. The device comprising a microfluidic chip having an oEWOD structure configured to create an optically-mediated electrowetting (oEWOD) force, the microfluidic chip includes a first region and a second region, wherein said first and second regions are separated by a constriction; wherein the first region is adapted to receive and manipulate one or more microdroplets dispersed in a carrier fluid at first flow rate; and wherein the second region is configured to receive the microdroplet via the constriction from the first region and transfer said microdroplet to an outlet port of the microfluidic chip in a second flow rate; wherein the second region is configured to receive said microdroplet via the constriction from the first region by application of an optically -mediated electrowetting (oEWOD) force; and wherein the second flow rate in the second region is higher than the first flow rate in the first flow region. A method and apparatus for dispensing one or more microdroplets are also provided.

Reusable network of spatially-multiplexed microfliuidic channels each including an inlet, an outlet, and a cuvette in-between. Individual channels may operationally share a main or common output channel defining the network output and optionally leading to a disposable storage volume. Alternatively, multiple channels are structured to individually lead to the storage volume. An individual cuvette is dimensioned to substantially prevent the formation of air-bubbles during the fluid sample flow through the cuvette and, therefore, to be fully filled and fully emptied. The overall channel network is configured to spatially lock the fluidic sample by pressing such sample with a second fluid against a closed to substantially immobilize it to prevent drifting due to the change in ambient conditions during the measurement. Thereafter, the fluidic sample is flushed through the now-opened valve with continually-applied pressure of the second fluid. System and method for photometric measurements of multiple fluid samples employing such network of channels.

Disclosed is an analysis system for analyzing water and wastewater, comprising an analysis device that includes a device housing which accommodates device components and which has an inlet on a housing surface, said inlet being designed as an injection port through which a substance to be analyzed can be introduced into a device component when the device housing is closed, and comprising a syringe that includes an injection needle outlet, the surface normal of which is congruent with the longitudinal axis; and/or the syringe includes an automatic ejection element for ejecting a predetermined amount of substance within a predetermined injection period.

A sample holder (10) comprises a sample chamber (33), a gas reservoir (32) and an upper layer (20) covering over the sample chamber (33) and gas reservoir (32), wherein a bottom surface of the upper layer (20) comprises a microstructure array (23) which overlies at least a portion of a top periphery of the sample chamber (33), and wherein the microstructure array (23) is in communication with a gas path which extends to the gas reservoir (32), to allow gas exchange between the sample chamber (33) and the gas reservoir (32).

Disclosed in the present invention are a digital nucleic acid amplification testing method and an integrated detection system based on CRISPR-Cas technology. The integrated detection system comprises an integrated reaction chip, a temperature control module, a light source and an optical signal detector. The method comprises: uniformly dividing a nucleic acid amplification reagent into amplification micro-droplets, then mixing the amplification micro-droplets after digital nucleic acid amplification with detection micro-droplets containing CRISPR-Cas detection reagent to perform a CRISPR reaction, and when the reaction is finished, detecting an optical signal to realize high-specificity testing of a target object, and the concentration or copy number of nucleic acid molecules in a sample to be tested is also obtained, and high-sensitivity absolute quantitative testing of a target object is realized.

A mixer for generating particles, comprising a first mixing unit, wherein the first mixing unit comprises a first channel (702) and a second channel (701), the first channel (702) comprises a rectilinear channel, the second channel (701) comprises a curvilinear channel. The mixer is particularly suitable for producing nanoparticles, and the mixing efficiency can be improved. A microfluidic hybrid chip cartridge prepared by the mixer is also provided.

A biomolecule analysis kit includes a reaction container configured to perform an enzymatic reaction, the reaction container including a base portion which has a container-shaped portion and a low-adsorption structural portion which is provided on at least the inner surface of the container-shaped portion, the low-adsorption structural portion having an adsorption rate lower than the base portion at which at least one of a sample which becomes a target of analysis in the enzymatic reaction and a reagent for the enzymatic reaction is adsorbed thereonto, wherein a signal resulting from the enzymatic reaction is configured to be detected when the enzymatic reaction is performed in the reaction container.

This invention is related to high-throughput screening field, in particular to an application method for automatic micro droplet array screening system of picoliter scale precision. According to this invention, the fluid driving system and the capillary are fully filled with fluid of low thermal expansion coefficient as the carrier fluid to thoroughly empty air bubbles in the capillary; after that, immersing the sampling end of capillary into the oil phase that is mutually immiscible with aqueous sample to aspirate a section of oil phase into the capillary for isolation of aqueous sample and carrier fluid; once completed, immersing the sampling end of capillary into the sample/reagent storage tube to aspirate a certain volume of aqueous sample into the capillary; finally, moving the sampling end of capillary to the oil phase above microwells on microwell array chip, and pushing the sample solution in the capillary into microwells to form sample droplet. Quantitative metering of fluid and droplet generation according to this invention are provided with volume precision in picoliter, which can effectively minimize the consumption of sample/reagent, and save the testing cost during high-throughput screening.

Disclosed herein are methods for producing high density cellular arrays. In some embodiments, the methods comprise: providing a sample comprising a plurality of cells; and introducing the plurality of cells in the sample into microwells of a microwell array to produce a cellular array, wherein the microwell array comprises 500 or more microwells per inch.sup.2, and wherein 25% or more of the microwells of the cellular array comprise a single cell. The disclosed methods can be used for producing a high density synthetic particle array and a high density reagent array.