The present invention relates to a means for improving fluid exchange across a microplate, comprising a microplate which comprises one or more internal modifications which aid the introduction and removal of fluids from the wells of the plate and minimize the damage caused to biological matter located therein upon introduction or removal of a pipette tip and/or fluids, and/or a means for inclining the microplate at an angle from the horizontal and retaining the microplate in the inclined position, to facilitate fluid introduction and aspiration from the wells of the microplate.

The invention provides for sequencing a nucleic acid molecule based on the detection of base incorporation by the release of pyrophosphate (PPi) using a new enzyme system comprising adenosine diphosphate (ADP)-glucose pyrophosphorylase (AGPase) and its substrate ADP-glucose.

Various methods and devices for spatial molecular analysis from tissue is provided. For example, a method of spatially mapping a tissue sample is provided with a microarray having a plurality of wells, wherein adjacent wells are separated by a shearing surface; overlaying said microarray with a tissue sample; applying a deformable substrate to an upper surface of said tissue sample; applying a force to the deformable substrate, thereby forcing underlying tissue sample into the plurality of wells; shearing the tissue sample along the shearing surface into a plurality of tissue sample islands, with each unique tissue sample island positioned in a unique well; and imaging or quantifying said plurality of tissue sample islands, thereby generating a spatial map of said tissue sample. The imaging and/or quantifying may use a nucleic acid amplification technique.

Methods, compositions, systems, apparatus, and kits are provided for depositing samples onto surfaces. The samples can include one or more particles, and the surface can include one or more reaction chambers. In some embodiments, the depositing can include the use of companion particles in combination with sample particles.

Supporting apparatus having a columnar first member and a cylindrical second member having an inner surface allowed to face with of an outer surface of the first member, one portion of the first member is inserted in a cylinder defined by the inner surface, a state of the members is allowed to change from a first state to a second state, the first state includes a state in which a predetermined space is ensured, the predetermined space is surrounded by one portion of the inner surface of the second member and an edge surface at one side of the first member that is different from the outer surface, the second state includes a state in which the second member has moved toward a side that is opposite to the one side relative to the first member from a base position of the members in the first state.

Systems, methods, and devices for analyzing small volumes of fluidic samples, as a non-limiting example, less than twenty microliters are provided. The devices are configured to make a first sample reading, for example, measure an energy property of the fluid sample, for example, osmolality, make a second sample reading, for example, detecting the presence or concentration of one or more analytes in the fluid sample, or make both the first sample reading and the second sample reading, for example, measuring the energy property of the fluid sample as well as detecting the presence or concentration of one or more analytes in the fluid sample.

The invention concerns a robotic method for coating the bottom surface of at least one well of a multiwell plate by a polyelectrolyte multilayer film, the multiwell plate obtainable according to the method and the use thereof for cell culture.

An exemplary embodiment of the present disclosure provides a fluidic device comprising a substrate, an applicator, and a spacer. The substrate can comprise a plurality of wells. The applicator can be used for manipulating a biological substance in at least a portion of the plurality of wells. The spacer can be positioned between the substrate and the applicator. The spacer can be configured to allow the applicator to apply the biological substance to at least a portion of the plurality of wells while maintaining a space between the substrate and the applicator.

A fluid ejection device may include a blank cassette that includes a substrate, a die coupled to the substrate, a number of assigned electrical traces formed on the substrate, and a number of unassigned electrical traces formed on the substrate. At least one wirebond may couple at least one of the unassigned electrical traces to the die thereby assigning at least one function to the fluid ejection device.

A cassette for retaining a specimen of surgically exposed tissue from a patient in an orientation that facilitates optical sectioning of the tissue by a confocal microscopic or other optical imaging microscope. The cassette includes a base member having a rigid optically transparent window upon which a tissue specimen is situated, a pliable membrane locatable over a substantial portion of the base member including the window, and an upper member, having an aperture therethrough, which can cover the base member to provide an enclosed cavity between the membrane and the window sealing the tissue specimen therein. The edges of the tissue specimen may be positioned planar against the window and retained in that position by bonds formed between the membrane and window at multiple points or locations around the tissue specimen. The specimen retained in the cavity is imagable by a microscope through the window of the base member.