Provided herein are magnetofluidic cartridges of use in a wide variety of sample analysis applications, including nucleic acid amplification assays. The magnetofluidic cartridges include sample inlet wells and sample analysis wells. Temperature sensitive materials are used to separate the sample inlet wells and sample analysis wells from one another prior to performing a given sample analysis application. Related magnetofluidic devices, kits, and methods are also provided.

The present invention provides a droplet generator. The droplet generator includes a large-corner continuous U-shaped flow channel on the encoded microsphere flow channel. Controlling the turning radius of the continuous U-shaped flow channel prevents the blockage resulted from accumulation of microfibers in the encoded microsphere suspension at the turn of the continuous U-shaped flow channel. A buffer tank is arranged at the encoded microsphere flow channel, which is used for the pre-arrangement of the encoded microspheres, to increase the controllability of the flow rate of the encoded microspheres. The buffer tank is provided in the oil-phase flow channel to prevent the oil phase from infiltrating into the aqueous phase due to excessive flow rate, and increase the controllability of the oil phase flow rate. The droplet generator has simple structure, low cost, high-throughput, and high-stability, and may be used to prepare single-cell single-encoded microsphere droplets.

A sampling apparatus can have an elongate tube and a liquid partitioning unit, first port can receive an incoming flow of test liquid and a second port may be coupled to the elongate tube. The liquid partitioning unit can combine the flow of test liquid with a plurality of partitioning elements to define a plurality of discrete liquid samples for movement along and storage in the elongate tube.

The present invention relates to a method for analyzing and selecting a specific droplet among a plurality of droplets (4), comprising the following steps: —providing a plurality of droplets (4), —for a droplet (4) among the plurality of droplets, measuring at least two optical signals, each optical signal being representative of a light intensity spatial distribution in the droplet for an associated wavelength channel, —calculating a plurality of parameters from the optical signals, —determining a sorting class for a droplet according to calculated parameters, —sorting said droplet according to its sorting class, wherein the plurality of parameters comprises the coordinates of a maximum for each optical signal and a co-localization parameter and the at least two calculated parameters used for the determining step comprises the co-localization parameter.

A detection chip, a using method for the same, and a reaction system. The detection chip includes a first substrate, a micro-cavity defining layer, and a heating electrode. The micro-cavity defining layer is on the first substrate and defines a plurality of micro-reaction chambers. The heating electrode is on the first substrate and is closer to the first substrate than the micro-cavity defining layer, and is configured to heat a plurality of micro-reaction chambers. The orthographic projection of the plurality of micro-reaction chambers on the first substrate is within the orthographic projection of the heating electrode on the first substrate.

The present disclosure provides a microchannel device including a base having a defining surface that defines a flow channel and containing silicone, in which the defining surface of the base includes a region in which a surfactant is adsorbed, and a ratio of an amount of secondary ions of the surfactant adsorbed on the defining surface of the base to a total amount of ions detected by time of flight secondary ion mass spectrometry is 0.01 or more, and provides a use application thereof.

The present invention provides methods and devices for simple, low power, automated processing of biological samples through multiple sample preparation and assay steps. The methods and devices described facilitate the point-of-care implementation of complex diagnostic assays in equipment-free, non-laboratory settings.

Disclosed are devices and methods useful for confined-channel digital microfluidics that combine high-throughput droplet generators with digital microfluidic for droplet manipulation. The present disclosure also provides an off-chip sensing system for droplet tracking.

The invention describes a method for the synthesis of compounds comprising the steps of: (a) compartmentalising two or more sets of primary compounds into microcapsules; such that a proportion of the microcapsules contains two or more compounds; and (b) forming secondary compounds in the microcapsules by chemical reactions between primary compounds from different sets; wherein one or both of steps (a) and (b) is performed under microfluidic control; preferably electronic microfluidic control, The invention further allows for the identification of compounds which bind to a target component of a biochemical system or modulate the activity of the target, and which is co-compartmentalised into the microcapsules.

A biochip; a liquid which has a different specific gravity from that of the reaction mixture and is immiscible with the reaction mixture; and an additive containing, as a principal component, a carbinol-modified silicone resin, a carboxyl-modified silicone resin, an amino-modified silicone resin, a polyether-modified silicone resin, a silanol-modified silicone resin, or a fluoro-modified silicone resin, wherein the vessel comprising, a flow channel that is capable of flowing a liquid droplet of a reaction mixture containing a surfactant in the longitudinal direction of the vessel.