Provided herein are methods and systems for biochemical analysis, including compositions and methods for processing and analysis of small cell populations and biological samples (e.g., a robotically controlled chip-based nanodroplet platform). In particular aspects, the methods described herein can reduce total processing volumes from conventional volumes to nanoliter volumes within a single reactor vessel (e.g., within a single droplet reactor) while minimizing losses, such as due to sample evaporation.

The invention relates to an array type paper chip for 2019-nCoV virus high-throughput detection and a manufacturing method of the array type paper chip. The array type paper chip comprises a glass substrate layer, a paper unit layer and a cell grid layer which are arranged in sequence from bottom to top, wherein the grid layer comprises N circular paper detection units with a diameter R being arranged in the form of an array; and the unit grids of the unit grid layer are in one-to-one correspondence to the paper detection units to separate the paper detection units. The array type paper chip is simple in structure, the manufacturing process is simple and stable, the finished products are stable, requirements on the processing environment and conditions are very low, and processing equipment is low in price. Moreover, the processing process does not revolve any chemical reagent, and therefore, the method is more environmentally friendly than methods such as ultraviolet lithography.

A rotary analysis system comprising: a rotary unit including a rotary platform, and having a TLC plate on which movement of a fluid sample and an eluent is controlled by rotational motion of the rotary platform, and aldehydes or ketones in the sample are separated and deployed with the eluent; a shooting unit for capturing an image of components of the sample separated by the rotary unit; a control unit for controlling the rotation conditions of the rotary unit and the capturing conditions of the shooting unit; and an analysis unit for analyzing the image captured by the shooting unit, and the rotary analysis system capable of economically and simply separating and detecting aldehydes or ketones.

Air-matrix digital microfluidics (DMF) apparatuses and methods of using them to prevent or limit evaporation and surface fouling of the DMF apparatus. In particular, described herein are air-matrix DMF apparatuses and methods of using them in which a separate well that is accessible from the air gap of the DMF apparatus isolates a reaction droplet by including a cover to prevent evaporation. The cover may be a lid or cap, or it may be an oil or wax material within the well. The opening into the well and/or the well itself may include actuation electrodes to allow the droplet to be placed into, and in some cases removed from, the well. Also described herein are air-matrix DMF apparatuses and methods of using them including thermally controllable regions with a wax material that may be used to selectively encapsulate a reaction droplet in the air gap of the apparatus.

A small volume aqueous sample followed by an air gap and a droplet of oil can be drawn into a pipette tip as a means of storing and protecting the sample from the environment for extended periods of time.

Multiple receptacles containing a reaction mixture are transported to corresponding receptacle wells of a thermally-conductive receptacle holder in thermal communication with a support that is in thermal communication with a heat sink. The heat sink is pre-heated to a temperature above ambient temperature, and the temperature of the receptacle holder is cycled. Optical communication is established between each of the receptacle wells and an excitation signal source and an emission signal detector during cycling, and whether an emission signal is emitted from any of the receptacles is determined as an indication of the presence of a target nucleic acid.

A microdevice for detecting aldehydes or ketones by utilizing a rotary platform, the microdevice comprising a disc-shaped rotary platform and a microfluidic structure disposed on the rotary platform, and the microfluidic structure comprising a sample storage part, an eluent storage part, a separation part, a first microfluid flow channel, a second microfluid flow channel, and an absorption pad.

A bottle compartment (1, 21) for an analyzer instrument, which bottle compartment comprises a frame (3) with a location (4) for a bottle (10, 23) of liquid, and a tube (7, 24) for aspiration of liquid from a bottle at the location for a bottle by the analyzer instrument, wherein the bottle compartment (1, 21) comprises a handle mechanism (2, 22) connected turnably to the frame (3), which handle mechanism in an open position allows access to the location (4) for insertion and removal of a bottle (10, 23) and in a closed position prevents access to the location, and that the tube (7, 24) for aspiration of liquid is connected to the handle mechanism so that when the handle mechanism is turned to the open position the end of the tube is simultaneously moved from the bottle at the location and when the handle mechanism is turned to the closed position the end of the tube is simultaneously moved inside the bottle at the location.

The present invention relates to a micro chamber plate, and more particularly, to a micro chamber plate having micro chamber holes formed using a flowable film. Thus, samples can be injected into the micro chamber holes in a smoother manner compared to when samples are injected using a vacuum and/or centrifugal force. In addition, bubbles and excess samples in the micro chamber holes can be efficiently discharged, making it possible to perform reaction and analysis in a more accurate and efficient manner.

A microfluidic device for analysing a test liquid comprises: a sensor (235), such as a membrane provided with nanopores, provided in a sensing chamber (237); a sensing chamber inlet channel (261) and a sensing chamber outlet channel (262), each connecting to the sensing chamber for respectively passing liquid into and out of the sensing chamber, and a reservoir (233) forming a sample input port to the microfluidic device, the reservoir being in fluid communication with the sensing chamber inlet channel (261); a liquid collection channel (232); a barrier (231) between an end of the sensing chamber outlet channel (262) and the liquid collection channel (232); a first seal (251), covering the sample input port; a second seal (252), covering the end of the sensing chamber outlet channel (262), thereby preventing liquid from flowing from the sensing chamber (237), over the barrier (231), into the liquid collection channel (232); wherein the microfluidic device is filled with a liquid from the first seal (251) at the sample input port to the second seal (252), such that the sensor (235) is covered by liquid and unexposed to a gas or gas/liquid interface; and wherein the first and second seals (251, 252) are removable to cause the liquid to flow between the reservoir and the end of the sensing chamber outlet and over the barrier.