A system and method for target material capture, the method comprising: receiving a set of target cells into an array of wells defined at a surface plane of a substrate; receiving a set of particles into the array of wells, thereby co-capturing the set of target cells and the set of particles; achieving a desired state for the array of wells upon receiving a washing fluid into a cavity in communication with the array of wells; receiving a lysis buffer into the cavity; receiving a partitioning fluid into the cavity, thereby displacing the lysis buffer from the cavity and partitioning each of the array of wells from adjacent wells, at the surface plane; and retaining intercellular material of the set of target cells, individually with the set of particles within the array of wells.

A biochip for the integration of all steps in a complex process from the insertion of a sample to the generation of a result, performed without operator intervention includes microfluidic and macrofluidic features that are acted on by instrument subsystems in a series of scripted processing steps. Methods for fabricating these complex biochips of high feature density by injection molding are also provided.

A method of performing an optical measurement of an analyte in a processed biological sample using a cartridge is provided. The cartridge is operable for being spun around a rotational axis. The method comprises: placing the biological sample into a sample inlet; controlling the rotational rate of the cartridge to process a biological sample into the processed biological sample using a fluidic structure; controlling the rotational rate of the cartridge to allow the processed biological sample to flow from the measurement structure inlet to an absorbent structure via a chromatographic membrane, and performing an optical measurement of a detection zone on the chromatographic membrane with an optical instrument. An inlet air baffle reduces evaporation of the processed biological sample from the chromatographic membrane during rotation of the cartridge.

A receptacle includes an upper portion having a cylindrical portion and an open end and a lower portion depending from the upper portion, where the lower portion is tapered and has a closed bottom end. The receptacle may include a radially-extending annular ring with a flat or sloped bottom surface and disposed on an outer surface of the receptacle at a transition between the upper portion and the lower portion and/or a radially-projecting lip circumscribing the open end, which may be configured for inter-locking engagement with a mated cap inserted into the open end of the upper portion. Embodiments may include one or more longitudinally-oriented grooves formed in an inner surface of the upper portion. The receptacle may be a unitary plastic structure.

A container assembly is provided for handling a biological sample that includes a first receptacle (1) and a second receptacle (2), each one configured to receive and contain a fluid; a coupling member (6), adapted to operably couple the first receptacle and the second receptacle, so as to provide a sealed fluid passage between the first and second receptacle; at least one self-sealing dispensing valve (4), operably mounted within said fluid passage of the coupling member, adapted to allow bi-directional fluid flow at a predetermined fluid pressure, and at least one barrier member (9), operably mounted within the fluid passage of the coupling member at an output of the first receptacle, configured to prevent solids of a predetermined size to pass from the first receptacle into the second receptacle.

A device for biological sample preparation and analysis is disclosed. The device includes a substrate and a plurality of spaced apart arrays disposed on an upper surface of the substrate. Each array includes a plurality of reaction vessels, each reaction vessel having a hydrophilic surface. A hydrophilic ring surrounds each array. Methods of making and using the device are also disclosed.

A cap having a lower portion, an upper portion with an opening formed therein, and a plurality of longitudinally oriented ribs disposed on an inner surface of the cap. The inner surface of the cap is defined by the opening formed in the upper portion of the cap, and each rib is associated with a concave recess disposed directly opposite the rib on an outer surface of the upper portion of the cap. The recess extends along at least part of the length of the rib.

Digital microfluidic (DMF) methods and apparatuses (including devices, systems, cartridges, DMF readers, etc.), and in particular DMF apparatuses and methods adapted for large volume. For example, described herein are methods and apparatuses for DMF using an air gap having a width of the gap that may be between 0.3 mm and 3 mm. Also described herein are DMF readers for use with a DMF cartridges, including those adapted for use with large air gap/large volume, although smaller volumes may be used as well.

The present invention is related to the field of bio/chemical sensing, assays and applications. Particularly, the present invention is related to collecting a small amount of a vapor condensate sample (e.g. the exhaled breath condensate (EBC) from a subject of a volume as small as 10 fL (femto-Liter) in a single drop), preventing or significantly reducing an evaporation of the collected vapor condensate sample, analyzing the sample, analyzing the sample by mobile-phone, and performing such collection and analysis by a person without any professionals.