A microfluidic device can include a microfluidic circuit that comprises an inlet port, a reagent-containing chamber configured to receive fluid from the inlet port, a non-aqueous-liquid-containing reservoir configured to receive liquid from the chamber, and a droplet-generating region configured to receive and produce droplets of liquid from the reservoir. The circuit can also include first and second valves or frangible members. The first valve or frangible member can have closed position in which fluid is prevented from entering or exiting the chamber therethrough and an open position in which fluid is permitted to enter or exit the chamber therethrough. The second valve or frangible member can have a closed position in which fluid is prevented from flowing between the chamber and the reservoir therethrough and an open position in which fluid is permitted to flow between the chamber and the reservoir therethrough.

Devices that includes a first portion, the first portion including at least one fluid channel; a fluid actuator; an analysis sensor disposed within the fluid channel; a conductivity sensor disposed within the fluid channel; and an introducer; a second portion, the second portion comprising: at least one well, the well containing at least one material, wherein one of the first or second portion is moveable with respect to the other, wherein the introducer is configured to obtain at least a portion of the material from the at least one well and deliver it to the fluid channel, and wherein the fluid actuator is configured to move at least a portion of the material in the fluid channel.

A system, configured to facilitate processing and detection of nucleic acids, the system comprising a process fluid container and a cartridge comprising: a top layer, a set of sample port-reagent port pairs, a shared fluid port, a vent region, a heating region, and a set of detection chambers; an intermediate substrate, coupled to the top layer comprising a waste chamber; an elastomeric layer, partially situated on the intermediate substrate; and a set of fluidic pathways, each formed by at least a portion of the top layer and a portion of the elastomeric layer, wherein each fluidic pathway is fluidically coupled to a sample port-reagent port pair, the shared fluid port, and a detection chamber, comprises a portion passing through the heating region, and is configured to be occluded upon deformation of the elastomeric layer, to transfer a waste fluid to the waste chamber, and to pass through the vent region.

A biochemical detection device is revealed. The biochemical detection device includes a cap and a base arranged at two ends of a test tube correspondingly and a driving member disposed on the base. A sample-mounting slot and a test-paper-mounting slot separated from each other are formed in the test tube. After sampling, a sample obtained is placed into the sample-mounting slot and mixed with an extraction solution therein. A thin-film on the sample-mounting slot is penetrated by the driving member to allow the extraction solution mixed with the sample flowing into a cavity of the test tube to react with a test strip in the test-paper-mounting slot. The test cost is reduced and the detection efficiency is improved due to simple structure and easy operation. Moreover, the sample and the extraction solution are sealed in the test tube to prevent environmental pollution caused by spread of viruses.

Broadly speaking, embodiments of the present techniques provide a liquid handling device that enables a user to more easily and efficiently perform sample dilutions, without requiring the user to perform any calculations or be in a controlled environment (e.g. a laboratory or sterile/aseptic environment). Advantageously, this may enable a user to perform sample dilutions and subsequent sample processing outside of a laboratory, such as during field work, or in environments, regions or countries where access to sterile/aseptic environments may be difficult or nonexistent. The device may be used to dilute any liquid sample, such as biological samples, chemical samples, or environmental samples (e.g. liquid samples taken from a river or lake, or soil samples that are mixed with liquid).

The object of the invention is a method of detecting genetic material in a biological sample in which the biological sample is loaded into the reaction cartridge (6) and then the reaction cartridge (6) is placed in the control device, the collected biological sample is taken to the isolation chamber (7), isolation of biological material from the tested sample by heating the isolation chamber (7), the isolated genetic material is moved into a plurality of reaction chambers (8.1, 8.2, 8.3, 8.4), genetic material is amplified by heating the reaction chambers (8.1, 8.2, 8.3, 8.4), lyophilized reagents for genetic material amplification together with lyophilized fluorescent tag intercalating with genetic material are present in the reaction chambers (8.1, 8.2, 8.3, 8.4), and signal detection from fluorescent tags is carried out along with the genetic material amplification stage.

The present invention discloses an electrophoretic chip comprising: (a) a non-conductive substrate designed to support elements of said electrophoretic chip; (b) an electrode structure for conducting current through said electrophoretic chip, printed on said non-conductive substrate and comprising a counter electrode and at least one working electrode, each electrode comprising a conductive low-resistance ink layer printed on the non-conductive substrate, and a carbon ink layer printed on top of and fully or partially covering said conductive low-resistance ink layer; (c) a dielectric ink insulator layer placed on top of, and covering, said electrode structure, said dielectric ink insulator layer having at least one opening above the counter electrode and at least one opening above said at least one working electrode, thereby forming at least one addressable location; and (d) a molecule capturing matrix spotted on and covering said at least one addressable location, thereby creating at least one microgel region.

An in-vitro diagnostic analyzer, a reagent card (10), and an installation structure (200) are disclosed. The installation structure (200) includes an installation body (210). The installation body (210) includes an installation hole (212) configured to sleeve a sample tube (70), a hollow needle (220), a sealing portion (240), and an air inlet channel (230). One end of the hollow needle (220) is capable of being inserted into the sample tube (70). The sealing portion (240) is in sealing fit with an outer wall of the sample tube (70). The air inlet channel (230) includes an air outlet hole (234) and an air inlet hole (232). The air outlet hole (234) is configured for communication with the sample tube (70) provided on the installation hole (212). The reagent card (10) is integrated with the installation structure (200), and the in-vitro diagnostic analyzer is integrated with the reagent card (10).

A releasing stopper for a container, the stopper including a first body configured to be directly coupled with the container and comprising a coupling element for allowing the removable fastening of the stopper on the container; a second body, axially movable with respect to the first body along a predefined axis of the stopper and cooperating with the first body for defining a reservoir at least temporarily insulated from the outer environment, a membrane or collapsible septum constituting at least a portion of the reservoir wall, openable and/or breakable for allowing the communication of the reservoir with the outer environment. The stopper includes a first closed configuration, wherein the reservoir is insulated from the outer environment, and a second open configuration, wherein the reservoir is in communication with the outer environment, and wherein the membrane or collapsible septum is opened by a perforation or opening element distinct from the stopper.

A collection device for a biological sample to capture target compounds such as viruses or other pathogens or particles for testing from within the sample and move the captured target compound to a separate chamber for subsequent processing. The collection device can include an openable substance blister including capture particles located in a cup interior. Capture particles can attract and bind the target compounds from the sample. An extraction tube extracts any nucleic acid from the target compound for storage or subsequent amplification and testing to confirm presence of known microorganisms. The extraction tube can comprise a heat-deformable material and can be connected to a microfluidic cartridge for further processing of nucleic acid including, amplification and detection. The microfluidic cartridge includes valves and a plurality of chambers for amplification.