Methods and systems for optofluidic device fabrication and design are herein disclosed. In examples, a user may manufacture the optofluidic device using stereolithography (SLA) three-dimensional (3D) printing, in which photosensitive resin is exposed to a focused laser, solidifying specific areas of resin. The optofluidic device may guide light for a broad wavelength range from a liquid or gas channel comprised within and may be used to guide the emission of light and particles of interest to a detector to identify the particles.

The present disclosure is drawn to loop-mediated isothermal amplification (LAMP) reaction assemblies including a substantially hygroscopic agent free LAMP reagent mixture in combination with a solid-phase reaction medium. The present disclosure also includes systems for a chromatic LAMP analysis including a substantially non-reactive solid phase reaction medium, and a non-interfering reagent mixture. The present disclosure also includes solid phase LAMP reaction mediums comprising a substrate, an adhesive layer disposed on the substrate, a reaction layer disposed on the adhesive layer, and a spreading layer disposed on the reaction layer. The present disclosure also includes methods of testing for a presence of a target nucleotide sequence including providing a biological sample, and dispensing the sample into a test environment having a solid phase reaction medium in combination with a LAMP reagent mixture and a pH sensitive dye.

A liquid biological sample test cartridge is disclosed. The cartridge can include a tray. The cartridge can also include a chemical reaction pad supported by the tray. The cartridge can further include a chemical reaction pad cover disposed over the chemical reaction pad and coupled to the tray. The chemical reaction pad cover can have a sample opening to facilitate depositing a liquid biological sample at a predetermined location on the chemical reaction pad. In addition, the cartridge can include an outer cover operable to at least partially form an enclosure about the chemical reaction pad.

The present disclosure is drawn to methods of preparing a saliva sample for loop-mediated isothermal amplification (LAMP) detection of a pathogen target. In some embodiments, such methods can include providing an amount of saliva from a test subject, and diluting the saliva in water to a degree that reduces a buffering capacity of the saliva while maintaining a sufficient concentration to allow for detection of the pathogen target.

A specimen cup holder and illumination device includes a first container, a second container, and a light source. The first container has a translucent bottom, opaque sides, an opaque top, and compartments defined therein. Each compartment includes a portion of the translucent bottom, portions of the opaque sides, and at least one opaque interior wall spanning between the translucent bottom and opaque top. The opaque top has specimen cup-receiving holes with each hole being in communication with one of the compartments. The second container has an open top and a closed bottom, and is configured to slidingly receive a portion of the first container therein so that the translucent bottom is suspended above the closed bottom. The light source is disposed in the second container between the translucent bottom of the first container and the closed bottom of the second container.

The present invention provides a detecting apparatus, including a storage chamber containing a treating fluid; wherein, the detecting apparatus is internally provided with a sharp-pointed portion; the storage chamber may make a movement relative to the sharp-pointed portion; the storage chamber will be pierced by the sharp-pointed portion during the moving process, such that the treating fluid in the storage chamber is released. The detecting apparatus further includes a collecting chamber; the released treating fluid may flow into the collecting chamber; the collecting chamber is disposed inside a first shell and used to contain a sample; an opening is disposed at an upper position of the first shell; the collecting chamber is internally provided with a testing element for detecting an analyte; the testing element is disposed on a carrier, and the carrier has a specific matching form with the collecting chamber. A buffer solution is disposed in an independent chamber of the detecting apparatus, and may be obtained at any time in need of detection and thus, is easy to be used. The carrier has a specific matching form with the collecting chamber in the first shell, such that the carrier has a definite and unique directional position after being inserted into the collecting chamber.

A technology is described for a system for identifying a colorimetric test result from a pathogen test performed on a solid phase substrate. The system can comprise a sensor configured to detect a spectrum of color wavelengths. The system can comprise one or more processors. The one or more processors can be configured to: receive color wavelength data; determine a wavelength threshold for providing a pathogen positive test result; identify whether the color wavelength data meets or exceeds the wavelength threshold for providing a pathogen positive test result; and generate a result indicator indicating either a pathogen positive or pathogen negative test result.

Described is a multi-dimensional double spiral (MDDS) microfluidic device comprising a first spiral microchannel and a second microchannel, wherein the wherein the first spiral microchannel and second spiral microchannel have different cross-sectional areas. Also described is a device comprising a multi-dimensional double spiral and system for recirculation. The invention also encompasses methods of separating particles from a sample fluid comprising a mixture of particles comprising the use of the multi-dimensional double spiral microfluidic device.

A bench top incubator is described. The bench top incubator includes a multiple temperature sensors and control systems so as to provide independent data logs of temperature data from each of the multiple temperature sensors. The incubator is relatively simple and small in design and can be conveniently located to carry out temperature processing of biological samples such as fixed cells and tissues, biological fluids, and so forth. The incubator can also include multiple tray stacks, each tray stack configured to retain either microscope slides or well plates

Blood sample collection and processing system (1) using a tube (102) and a holder (104) is described herein. The tube (102) includes a plasma separation system (114) for converting a whole blood sample into a plasma sample without using centrifugation. The plasma separation system (114) may comprise a microfluidic separation system such as a lateral cavity acoustic transducer system (113). The tube (102) is thereafter placed in the holder (104) for analysis regarding the plasma. The results of the analysis may be displayed to the user via a display screen (134) or an indicator of a different kind, to allow the user to determine whether the sample is sufficient with regards to volume, and/or quality for further use. Inasmuch as the analysis system (132) performs a rapid analysis of the plasma sample, the patient is still present and if the sample is insufficient, the healthcare provider can take another whole blood sample, counsel the client regarding the blood sample, or examine the patient further.