A low-cost method is provided for fabricating a nano-droplet plate with surface features having re-entrant (anvil-like) geometries capable of holding droplets of a precise, predetermined volume. Such structures are useful for a variety of applications, including cull culturing, high-throughput screening of therapeutics and as microwells.

A microfluidic system for cell retention for a perfusion bioreactor is provided. The system comprises at least one inlet configured to receive a bioreaction mixture to be processed. At least one curvilinear microchannel is in fluid flow connection with the at least one inlet, the at least one curvilinear microchannel being adapted to isolate cells in the bioreaction mixture, based on cell size, along at least one portion of a cross-section of the at least one curvilinear microchannel. At least two outlets are in fluid flow connection with the at least one curvilinear microchannel. At least one outlet of the at least two outlets is configured to flow the isolated cells to be recycled to the perfusion bioreactor.

The present disclosure relates to a fluid analysis device which comprises a sensing device for analyzing a fluid sample, the sensing device comprising a micro-fluidic component for propagating the fluid sample and a microchip configured for sensing the fluid sample in the micro-fluidic component; a sealed fluid compartment containing a further fluid, the compartment being fluid-tight connected to the sensing device and adapted for providing the further fluid to the micro-fluidic component when the sealed fluid compartment is opened; and an inlet for providing the fluid sample to the micro-fluidic component. Further, the present disclosure relates to a method for sensing a fluid sample using the fluid analysis device.

A reinforced microplate has reinforcing members that enhance stiffness and minimize deformation of the microplate, especially thermally-induced deformation. The reinforcing members include ribs or struts that are integrally formed on a bottom surface of the microplate. In cooperation with the reinforcing members, the microplate frame can include one or more slots that act to disrupt the effects of thermal expansion and limit thermally-induced strain.

The present invention provides a fluid inspection device comprising at least one channel including a top channel surface and a bottom channel surface, and a first spacing formed therebetween; at least one chamber communicating with the at least one channel from which a fluid flows into the at least one chamber and including a top chamber surface, a bottom chamber surface and a fluid-filling area and a through opening communicating with the at least one chamber and the outside. A second spacing is formed between the top chamber surface of at least one portion of the fluid-filling area and a corresponding bottom chamber surface thereof, wherein the second spacing is smaller than the first spacing. The fluid inspection device prevents bubbles from producing in the chamber and obstructing the inspection and further allows less required fluid amount in the chamber.

The present invention relates to a device and a method for separating bubbles from a fluid comprising a chamber through which a fluid can pass. According to the invention the device is characterized in that the inner chamber wall has a geometry that generates within said chamber a continuous flow and at least one area with a discontinuous flow which has a low flow velocity so that bubbles remain at the inner chamber wall in said area and thus are separated from the fluid flowing out from the chamber.

[Problem to be solved] A sample storage tube having the seal function to the tube body is provided by integral molding and the airtightness is secured in the sample storage tube.

[Solution] The sample storage tube 100 comprise a first molded portion 210 and a second molded portion 220 by the integral molding. The first molded portion 210 is molded as the base figure of the tube body. The second molded portion 220 includes at least the opening edge portion which contacts the lid 300 and closes the opening. The first molded portion 210 is molded by the first material such as polypropylene, the second molded portion 220 is molded by the second material such as TPE which is suitable for the seal object to secure the airtightness between the opening and the lid 300. This second molded portion providing the seal function is molded onto the opening edge of the tube body by the integral molding. The second molded portion 220 can be molded as a consecutive object from the bottom portion to the opening edge of the tube body.

A microfluidic apparatus is provided having one or more sequestration pens configured to isolate one or more target micro-objects by changing the orientation of the microfluidic apparatus with respect to a globally active force, such as gravity. Methods of selectively directing the movements of micro-objects in such a microfluidic apparatus using gravitational forces are also provided. The micro-objects can be biological micro-objects, such as cells, or inanimate micro-objects, such as beads.

The invention relates to a connection system for connecting an inside environment of a compartment to an environment outside said compartment, said compartment being isolated relative to the outside environment and said connection system, said connection system comprising a blocking device.

A multiwell plate cover is provided, wherein the cover comprises a frame configured for attachment to a multiwell plate; a first movable guide member having two elongated side members that extend across the width of the plate, wherein a gap between the side members is substantially equal to a diameter of a single well in the multiwell plate and wherein the first movable guide member is slidable along the length of the frame; and a second movable guide member having two elongated side members that extend across the length of the plate, wherein a gap between the side members is substantially equal to a diameter of a single well in the multiwell plate and wherein the second movable guide member is slidable along the width of the frame, wherein the first and second movable guide member are configured to move independently from each other.