A microfluidic device for and methods of using surface-attached posts and capture beads in a microfluidic chamber is disclosed. For example, the microfluidics device includes a pair of substrates separated by a gap and thereby forming a reaction (or assay) chamber therebetween. A field of actuatable surface-attached posts (e.g., magnetically responsive microposts) is provided on one or both of the substrates. The surface-attached posts are functionalized with capture beads. Additionally, methods are provided of functionalizing the surface-attached posts with the capture beads. Additionally, methods are provided of using the surface-attached posts that are functionalized with capture beads in a microfluidics device for binding a target of interest. Further, a bead spraying system and method is provided for spraying magnetically responsive and/or non-magnetically responsive beads atop and/or among a field of surface-attached microposts for use in a microfluidic device.

A microfluidic device includes a bottom electrode, a dielectric layer on the bottom electrode, one or more top electrodes on a region of the dielectric layer, Each of the one or more top electrodes has a sidewall that forms a sidewall angle with an outer surface of the dielectric layer that is less than 180 degrees. The sidewall of each of the one or more top electrodes and a portion of the outer surface of the dielectric layer adjacent to the sidewall define a microchannel region for transporting an open microchannel of a fluid. Such microfluidic devices may enable transport of small microchannels using low voltages.

A fluidic device (1) comprises a flow chamber (2) for accommodating a biological specimen on a carrier portion (3) and at least one flow channel (4a, 4b, 4c, 4d) fluidly connected to the flow chamber (2), the fluidic device (1) having a layered structure comprising a bottom plate (5), a cover plate (6) and an insert (7) in between, the insert (7) comprising the carrier portion (3) and a frame portion surrounding the carrier portion (3), and being elastomeric in order to be able to clamp a biological specimen between an incision in the carrier portion (3).

In a method for producing a compound of at least two polymer substrates, two polymer substrates each having a connecting surface are provided. At least one of the polymer substrates is coated with a self-assembling polypeptide, at least in the area of the connecting surface. The two polymer substrates are connected by pressing together the connecting surfaces under pressure and at a temperature corresponding to at least the glass transition temperature of the material of one of the polymer substrates at the connecting surface, wherein a diffusion of polymer chains takes place between the connecting surfaces by the self-assembling polypeptide and a solid connection is formed between the two connecting surfaces. A sealed microfluidic cartridge includes a polymer cartridge and a sealing film connected by such a method.

A test strip (12) includes a flow path (26) formed in a main body portion (20); a reagent portion (22b) provided in the flow path (26); and an intake portion (24) which is provided at a starting end of the flow path (26) and through which a sample is introduced into the flow path (26). The main body portion (20) is provided with a buffer space (28) communicating with a terminal end of the flow path (26), and a vent hole (30) opened at an outer surface of the main body portion (20) and communicating with the buffer space (28), and in a region where the buffer space (28) and the flow path (26) are connected, a cross-sectional area (Sb) of the buffer space (28) is larger than a cross-sectional area (S) of the flow path (26).

An analysis cartridge includes a first cover, a second cover, a plurality of containers, a plurality of fluid tunnels and a rotary valve. The second cover has two opposite surfaces, a plurality of first through holes and a second through hole individually penetrate through the two opposite surfaces, and the first cover is attached to the second cover. The plurality of containers are disposed between the first cover and the second cover, with each of the containers being aligned to and filled in the first through holes. The plurality of the fluid tunnels are disposed on the first cover, and each of which is individually connected with a first pipette. The rotary valve is rotatably disposed between the first cover and the second cover to correspond to the second through hole, and a flow channel disposed on the rotary valve is connected with the containers individually.

Disclosed is an apparatus and method of forming, including a supporting structure, a sensor on the supporting structure, a pair of columns on the supporting structure at opposite sides of the sensor, the pair of columns having a column height relative to a top surface of the supporting structure, the column height being higher than a height of the active surface of the sensor relative to the top surface of the supporting structure, and a lidding layer on the pair of columns and over the active surface, the lidding layer being supported at opposite ends by the pair of columns. The active surface of the sensor, the lidding layer and the pair of columns form an opening above at least more than about half of the active surface of the sensor, and the supporting structure, the sensor, the lidding layer and the pair of columns together form a flow cell.

Disclosed are a biosensing test strip (100, 200, 300, 500, 600, 700, 800, 900, 1000, 1100) and a biosensing test method. The biosensing test strip (100, 200, 300, 400, 500, 600, 700, 800, 900, 1000, 1100) comprises: a reaction layer (120, 220, 720, 820) provided with a reaction flow channel (121, 221, 821, 920, 1020); a partition plate layer (130, 230) located above the reaction layer (120, 220, 720, 820) and covering the reaction flow channel (121, 221, 821, 920, 1020); an exhaust layer (140, 240, 540, 640) located above the partition plate layer (130, 230), with the exhaust layer (140, 240, 540, 640) being provided with an exhaust flow channel (141, 241, 550, 650); and a communication hole passing through the partition plate layer (130, 230) to enable the exhaust flow channel (141, 241, 550, 650) to be in communication with the reaction flow channel (121, 221, 821, 920, 1020).

A microfluidic sensor for the detection of analytes in objects includes a contact surface that may be attached to a surface of the object, an inlet hole in the contact surface for the entry of fluids emitted by the object, and a first reservoir which stores an ionic fluid in the form of a polymer matrix. The polymer matrix includes a reactive substance which changes colour when it enters into contact with the analytes of the fluids emitted by the object. It further includes at least one first microfluidic duct which connects the inlet hole to the first reservoir. A system for the detection of analytes, a method for the manufacture of the microfluidic sensor and the use of the microfluidic sensor for the detection of analytes in works of art are also related.

A flow stabilized chip includes a chip mainbody, a buffering chamber and two fluid delivery ports. The chip mainbody has a pipe-connection surface. The buffering chamber is disposed in the chip mainbody. The two fluid delivery ports are disposed on the pipe connection surface and connected to the buffering chamber. The chip mainbody includes, in order from the pipe-connection surface to a bottom of the chip mainbody, a first base plate, a first elastic membrane, a second base plate, a second elastic membrane and a third base plate. The first base plate includes a first opening. The second base plate includes a second opening. The third base plate includes a third opening. The first elastic membrane, the second base plate and the second elastic membrane are stacked in sequence to form the buffering chamber.