A method and system for improving throughput of a fluidic system such as a biopolymer analysis system by cleaning accumulated or clogging biopolymer from the fluidic system is disclosed. The method and system utilize a light energy source to photocleave the biopolymer molecules that may accumulate or aggregate in the fluidic system or clog a passageway. The accumulated biopolymer may be exposed to a light energy source for a sufficient period of time such that the biopolymer molecule is dosed with sufficient energy to photocleave the biopolymer molecules, thereby restoring the efficiency of and flow through the system.

A plasmon resonance system, instrument, cartridge, and methods for analysis of analytes is disclosed. A PR system is provided that may include a DMF-LSPR cartridge that may support both digital microfluidic (DMF) capability and localized surface plasmon resonance (LSPR) capability for analysis of analytes. In some examples, the DMF portion of the DMF-LSPR cartridge may include an electrode arrangement for performing droplet operations, whereas the LSPR portion of the DMF-LSPR cartridge may include an LSPR sensor. In other examples, the LSPR portion of the DMF-LSPR cartridge may include an in-line reference channel, wherein the in-line reference channel may be a fluid channel including at least one functionalized LSPR sensor (or sample spot) and at least one non-functionalized LSPR sensor (or reference spot). Additionally, methods of using the PR system for analysis of analytes are provided.

This disclosure provides a gene chip comprising a substrate and at least one positioning device fixed on an upper surface of the substrate, wherein the at least one positioning device is provided with a receiving cavity for receiving a bead, the receiving cavity being arranged on a surface of the at least one positioning device facing away from the substrate, and a cross-sectional area of the receiving cavity is gradually decreased in a direction toward the upper surface of the substrate. This disclosure further provides a gene detection device comprising the gene chip.

The present invention relates to an apparatus, i.e. a device, for transferring a representative sample of a liquid containing particles into a chamber for analysis, such as for example, whole anticoagulated blood. The apparatus comprises an ante-chamber configured for receiving, containing, and transferring a portion of the sample to an analysis chamber. The chambers are continuous with each other, configured to allow the capillary flow of substantially the entirety of the contents of the ante-chamber into the analysis chamber to under-fill or substantially fill the analysis chamber. The apparatus can be conveniently configured as a disposable, single-use device, such as a dipstick, for performing both sampling and analysis from a single apparatus. The apparatus is useful for providing reliable and reproducible blood and body fluid analyses.

An integrated system for automatically analyzing in real time an analyte in a sample containing fluid is disclosed. The system includes a fluid separator for receiving the sample and separating therefrom a fluid component that contains the analyte, a non-optical, chemical analyte sensing device having at least one sensor for chemically analyzing the analyte, and a microfluidic channel for transferring at least a portion of the fluid component from the separator to the sensing device. In a preferred embodiment, the system is point of care, single-use cartridge in a base unit that separates plasma from a small sample of whole blood and tests an analyte of interest in the plasma.

Microfluidic devices and methods thereof; the devices including: SNDA components; each SNDA component comprising: a primary channel; secondary channels; and nano-wells that are each open to the primary channel and are each connected via vents to the secondary channel; the vents are configured to enable passage of gas solely from the nano-wells to the secondary channel, such that when a fluid is introduced into the primary channel it fills the nano-wells, and the originally accommodated gas is evacuated via the vents and the secondary channel/s; a common inlet port, configured to enable a simultaneous introduction of the fluid into all the primary channels of the different SNDA components; individual inlet ports, configured to enable individual introduction of fluid, each into a different primary channel of a different SNDA component; and at least one outlet port, configured to enable evacuation of the gas out of all the secondary channels.

Disclosed is an assay device comprising a high density of wells aligned thereon.

For example, a flowcell includes: a nanowell layer having a first set of nanowells and a second set of nanowells to receive a sample; a first linear waveguide associated with the first set of nanowells, and a second linear waveguide associated with the second set of nanowells; and a first grating for the first linear waveguide, and a second grating for the second linear waveguide, the first and second gratings providing differential coupling of first light and second light.

Disclosed in one example is an apparatus including a substrate, a sensor over the substrate including an active surface and a sensor bond pad, a molding layer over the substrate and covering sides of the sensor, the molding layer having a molding height relative to a top surface of the substrate that is greater than a height of the active surface of the sensor relative to the top surface of the substrate, and a lidding layer over the molding layer and over the active surface. The lidding layer and the molding layer form a space over the active surface of the sensor that defines a flow channel.

A blocking material is injected into a microfluidic chip that includes microscale-porosity microchannels etched in a substrate, filling at least a portion of the microchannels. Silicon dioxide spheres are injected into the microfluidic chip. The blocking material prevents the silicon dioxide spheres from entering the portion of the microchannels filled with the blocking material. The silicon dioxide spheres form a region of nanoscale porosity in a portion of the microchannels not filled with the blocking material. A solvent is injected into the microfluidic chip, the solvent operable to dissolve the blocking material and thereby providing a region of microscale porosity adjacent to the region of nanoscale porosity.