A fluidic chip comprising: a sealing layer having an upper surface and a lower surface; and a formed part comprising a generally planar body having a lower surface sealed with the upper surface of the sealing layer, the generally planar body having a number of through holes and a number of wells in fluid communication with the number of through holes, wherein together with the upper surface of the sealing layer, the number of through holes and the number of wells respectively define a number of fluid inlets and a number of fluid chambers in fluid connection with each other in the fluidic chip.

Provided are methods, devices, and kits for the isolation of extracellular vesicles using silicon nanomembranes. A method for EV isolation includes the steps of collecting a biofluid sample, contacting the biofluid sample with a pre-filtration membrane, thereby forming a first filtrate and a first retentate, optionally, washing the first retentate of the pre-filtration membrane, contacting the first filtrate from the pre-filtration membrane with a capture membrane, thereby forming a second filtrate and a second retentate, optionally, washing the second retentate, and eluting the second retentate from the capture membrane or lysing the second retentate to recover the contents.

A sample collection device is composed of a conductive polymer. The conductive polymer includes a mixture of carbon nanotubes and a polymer. The sample collection device has a hole at a tip of the sample collection device with the hole having a size ranging from about 0.15 mm to about 0.25 mm.

A method for fabricating calcite channels in a nanofluidic device is described. A porous membrane is attached to a substrate. Calcite is deposited in porous openings in the porous membrane attached to the substrate. A width of openings in the deposited calcite is in a range from 50 to 100 nanometers (nm). The porous membrane is etched to remove the porous membrane from the substrate to form a fabricated calcite channel structure. Each channel has a width in the range from 50 to 100 nm.

Disclosed herein, inter alia, are nanoarrays and methods of use thereof.

A blocking material is injected into a microfluidic chip that includes microscale-porosity microchannels etched in a substrate, filling at least a portion of the microchannels. Silicon dioxide spheres are injected into the microfluidic chip. The blocking material prevents the silicon dioxide spheres from entering the portion of the microchannels filled with the blocking material. The silicon dioxide spheres form a region of nanoscale porosity in a portion of the microchannels not filled with the blocking material. A solvent is injected into the microfluidic chip, the solvent operable to dissolve the blocking material and thereby providing a region of microscale porosity adjacent to the region of nanoscale porosity.

A fluidic chip includes at least one nanochannel array, the nanochannel array including a surface having a nanofluidic area formed in the material of the surface; a microfluidic area on said surface; a gradient interface area having a gradual elevation of height linking the microfluidic area and the nanofluidic area; and a sample reservoir capable of receiving a fluid in fluid communication with the microfluidic area. In another embodiment, a fluidic chip includes at least one nanochannel array, the nanochannel array includes a surface having a nanofluidic area formed in the material of the surface; a microfluidic area on said surface; and a gradient interface area linking the microfluidic area and the nanofluidic area, where the gradient interface area comprises a plurality of gradient structures, and the lateral spacing distance between said gradient structures decreases towards said nanofluidic area; and a sample reservoir capable of receiving a fluid in fluid communication with the microfluidic area.

An example of an array includes a support including a plurality of discrete wells, a gel material positioned in each of the discrete wells, a sequencing primer grafted to the gel material, and a non-sequencing entity grafted to the gel material. Each of the sequencing primer and the non-sequencing entity is in its as-grafted form.

Techniques regarding one or more structures that can facilitate automated, multi-stage processing of one or more nanofluidic chips are provided. For example, one or more embodiments described herein can comprise a system, which can comprise a roller positioned adjacent to a microfluidic card comprising a plurality of fluid reservoirs in fluid communication with a plurality of nanofluidic chips. An arrangement of the plurality of nanofluidic chips on the microfluidic card can defines a processing sequence driven by a translocation of the roller across the microfluidic card.

Devices and methods for the detection of nucleic acids (e.g., RNA, DNA) are described. The nucleic acid can be obtained or derived from a pathogen, such as a virus. In one embodiment, the virus is a coronavirus (e.g., SARS-CoV-2) related to the disease COVID-19. Accordingly, devices and methods may be used in the field as point-of-care devices to test a subject (e.g., a patient) for the presence of the SARS-CoV-2 virus or another nucleic acid.