The present exemplary embodiments provide a sample separation device which applies an electric field to a selective ion permeable layer based on origami to concentrate a target material in a specific area and concentrates a target material and separates a non-target material through a filter layer in which a paper is compressed to adjust a size of micro pore.

A capillary driven microfluidic system and a biosensing device including the capillary driven microfluidic system are provided. The capillary driven microfluidic system includes: a first substrate comprising at least one microfluidic channel ending in an opening, and having, adjacent to the opening, a protruding element; and a second substrate comprising at least one open cavity. The at least one protruding element and the at least one cavity include at least one hydrophilic surface. In addition, the at least one protruding element and the at least one cavity may be adapted for engaging with one another for providing transfer of a fluid between the first substrate and the second substrate. A space between the at least one hydrophilic surface of the at least one protruding element and the at least one hydrophilic surface of the at least one cavity is provided, where the separation between said surfaces is such that capillary forces are generated on the fluid upon entering inside the space.

This invention relates to compositions of matter, methods, modules and automated, end-to-end closed instruments for automated mammalian cell growth, reagent bundle creation and mammalian cell transfection followed by nucleic acid-guided nuclease editing in live mammalian cells. The disclosed compositions and method entail making “reagent bundles” comprising many (hundreds of thousands to millions) clonal copies of an editing cassette and delivering or co-localizing the reagent bundles with live mammalian cells such that the editing cassettes edit the cells and the edited cells continue to grow.

A system and method for receiving and delivering a fluid, the system comprising: a body configured to interface with an opening of a reservoir and defining: a protrusion defining a set position of the body relative to the reservoir; a wall extending from the protrusion; a receiving surface coupled to the wall and sloping from an apex to a nadir along a first direction, the receiving surface comprising a vent; and an outlet positioned closer to the nadir than the apex of the receiving surface and displaced from the vent, the outlet comprising an extension from the body, the extension configured to contact an interior wall of the reservoir, wherein the body comprises: a bubble-mitigating operation mode in which the receiving surface receives and transmits the fluid along the receiving surface, and a fluid-transmitting operation mode in which the body directs the fluid along the interior wall of the reservoir.

A method for moving an aqueous droplet comprising providing an electrokinetic device including a first substrate having a matrix of electrodes, wherein each of the matrix electrodes is coupled to a thin film transistor, and wherein the matrix electrodes are overcoated with a functional coating comprising: a dielectric layer in contact with the matrix electrodes, a conformal layer in contact with the dielectric layer, and a hydrophobic layer in contact with the confornial layer; a second substrate comprising a top electrode; a spacer disposed between the first substrate and the second substrate and defining an electrokinetic workspace; and a voltage source operatively coupled to the niatrix electrodes. The method further comprises disposing an aqueous droplet on a first matrix electrode; and providing a differential electrical potential between the first matrix electrode and a second matrix electrode with the voltage source, thereby moving the aqueous droplet.

A system and method for receiving and delivering a fluid, the system comprising: a body configured to interface with an opening of a reservoir and defining: a protrusion defining a set position of the body relative to the reservoir; a wall extending from the protrusion; a receiving surface coupled to the wall and sloping from an apex to a nadir along a first direction, the receiving surface comprising a vent; and an outlet positioned closer to the nadir than the apex of the receiving surface and displaced from the vent, the outlet comprising an extension from the body, the extension configured to contact an interior wall of the reservoir, wherein the body comprises: a bubble-mitigating operation mode in which the receiving surface receives and transmits the fluid along the receiving surface, and a fluid-transmitting operation mode in which the body directs the fluid along the interior wall of the reservoir.

A microfluidic product utilizing gradient surface energy coatings for fluid control comprising a plurality of fluid passages wherein at least one fluid passage comprises a coating configured to control liquid flow wherein the coating configured to control liquid flow comprises a gradient surface energy coating from a proximal location to a distal location on a surface of the fluid passage. The product can include uniform regions and surface gradient regions in the same passage. Coating compositions and product dimensions can be selected to provide control over different flow properties including fluid velocity, reduction and acceleration of fluid flow, and starting and stopping fluid flow.

Superparamagnetic nanoparticle-based analytical method comprising providing a sample having analytes in a sample matrix, providing a point of care chip having analytical regions, each of which is a stationary phase having at least one or more sections, labeling each of the analytes with a superparamagnetic nanoparticle and immobilizing the labeled analytes in the stationary phase, providing an analytical device having a means for exciting the superparamagnetic nanoparticles in vitro and a means for sensing, receiving, and transmitting response of the excited superparamagnetic nanoparticles, placing the chip in the analytical device and exciting the superparamagnetic nanoparticles in vitro, sensing, receiving, and transmitting the response of the superparamagnetic nanoparticles, and analyzing the response and determining characteristic of the analytes, wherein the response of the superparamagnetic nanoparticles comprises harmonics. The present invention also provides the hybrid point of care chip and analyzer to be used in the analytical method.

A digital microfluidic chip, a method for driving the same, and a digital microfluidic device are provided. The digital microfluidic chip includes a state transition layer configured to bear a droplet, and a light driving layer configured to provide light for controlling a lyophobicity-lyophobicity transition of the state transition layer to drive the droplet to move. The light driving layer includes light emitting units arranged in an array and provides light. The state transition layer realizes a lyophobicity-lyophobicity transition. The light driving layer controls the lyophobicity-lyophobicity transition by providing light to drive the droplet to move. An existing digital microfluidic chip has a complex structure and a high fabricating cost, while the digital microfluidic chip of the present disclosure has a simple structure, a simple fabricating process and a low fabricating cost, and can realize miniaturization and integration to a maximum extent.

A device of nondestructive transfer of liquid drops includes a power generation part and a clamping part. The power generation part includes a movable friction material and at least two fixed friction materials. The clamping part includes a supporting mechanism and a left dielectric wetting splint and a right dielectric wetting splint installed on the supporting mechanism. The movable friction material is connected to the left dielectric wetting splint. The at least two fixed friction materials are connected to the right dielectric wetting splint. Also disclosed are a method of nondestructive transfer of liquid drops and a method of micro-reaction of liquid drops.