A sample carrier may include a sample preparation module and an amplification module. A sample mixes with a lysis medium and a nucleic acid amplification medium in the sample preparation module and then flows into a plurality of microfluidic chambers in the amplification module. The microfluidic chambers have disposed therein primers configured to initiate amplification of one or more target nucleic acid sequences corresponding to one or more pathogens. The sample carrier is inserted into an apparatus that includes a plurality of Sight sources and a camera. The light sources illuminate the microfluidic chambers with excitation light, a fluorophore emits fluorescence light indicative of nucleic acid amplification in response to the excitation-light, and the camera captures images of the microfluidic chambers. A target nucleic acid sequence in the sample is indicated by the images showing an increasing fluorescence in a microfluidic chamber that has the primers for that sequence.

The present invention provides a system and method for building and optimizing biosensors to create a multi-layered bio-sensing system by incorporating two different formats comprised of i) a single wall carbon tubular sensing element and ii) a non-tubular graphene sensing element. This multi-layered system allows for assaying molecules across a large range of molecular weights by sensing molecules in both gas and liquid from a common sample simultaneously. By collecting and analyzing both larger, heavier molecules, including, but not limited to: proteins hormones, nucleic acids, lipids, lipoproteins, etc., with non-tubular graphene sensors and smaller, lighter volatile organic compounds (VOCs) as emitted in gas form from the same sample are assayed with single walled-carbon nanotubules (SWNTs), this invention provides a more complete, holistic understanding of the organism's current state of health.

Among other things, the present invention is related to devices and methods of performing biological and chemical assays, particularly an easy sample manipulation and/or a rapid change or a rapid thermal cycling of a sample temperature is needed (e.g. Polymerase Chain Reaction (PCR) for amplifying nucleic acids).

A microfluidic preconcentrator is provided, designed to receive a gas sample containing gaseous pollutants such as volatile organic compounds, to concentrate the gaseous pollutants and to transfer them to an analysis device. An assembly comprising an enclosure, a microfluidic preconcentrator, connectors and a means for holding the microfluidic preconcentrator inside the enclosure, a heating device and a cooling device, are provided.

Provided herein are systems and methods for a point of need testing system. In some embodiments, the point of need testing system includes a first collector and a sample amplifier. The first collector is configured to collect a first biofluid sample. The sample amplifier includes an inlet dimensioned to receive the first biofluid sample. The sample amplifier further includes a filter, a heater, and a second collector. In some embodiments, a reader can be used with the sample amplifier to read a second biofluid produced by the sample amplifier.

Systems and methods for conducting designated reactions utilizing a base instrument and a removable cartridge. The removable cartridge includes a fluidic network that receives and fluidically directs a biological sample to conduct the designated reactions. The removable cartridge also includes a flow-control valve that is operably coupled to the fluidic network and is movable relative to the fluidic network to control flow of the biological sample therethrough. The removable cartridge is configured to separably engage a base instrument. The base instrument includes a valve actuator that engages the flow-control valve of the removable cartridge. A detection assembly held by at least one of the removable cartridge or the base instrument may be used to detect the designated reactions.

Provided herein are magnetofluidic cartridges of use in a wide variety of sample analysis applications, including nucleic acid amplification assays. The magnetofluidic cartridges include sample inlet wells and sample analysis wells. Temperature sensitive materials are used to separate the sample inlet wells and sample analysis wells from one another prior to performing a given sample analysis application. Related magnetofluidic devices, kits, and methods are also provided.

The present invention relates to an automatic real-time quantitative amplification system which can perform analysis of various biological samples, and more particularly to an automatic real-time quantitative amplification system in which a plurality of decks for respectively accommodating biological samples are put in a deck storing/transferring device, whereby it is possible to automatically analyze an amount or existence of a target substance containing a target nucleic acid in the biologic sample, such as a particular gene, a particular, a particular pathogenic bacterium and a particular protein, by amplifying the target nucleic acid purified by some processes of purification, purification after culture, or purification after reaction of the target substance contained in the biological sample and then checking an amount of the amplified target nucleic acid.

A microfluidic device includes a channel through which a reaction solution flows. The channel passes through a reaction section having a plurality of temperature zones set at predetermined different temperatures. The channel includes, at least in the reaction section, a region where a cross-sectional area decreases in a feeding direction of the reaction solution.

The invention relates to a method for tempering a plurality of capillaries, which are arranged on a carrier, wherein the carrier having a length, width and height receives the capillaries along the width of the carrier. The carrier has a recess in order to receive a tempering element so that the capillaries may be tempered in their central region by means of contact with the tempering element. According to the invention, the ends of the capillaries filled with samples are unsealed during tempering.