Embodiments include a reaction vessel having a first reaction chamber filled with a first material; a first light absorbing region adhered to an interior-facing surface of the first reaction chamber; a second reaction chamber filled with a second material; a second light absorbing region adhered to an interior-facing surface of the second reaction chamber; a temperature sensor disposed within the second reaction chamber; and one or more energy sources configured to direct light at the first light absorbing region and the second light absorbing region. A processor may be employed to determine a first temperature of the first material from a second temperature of the second material measured by the temperature sensor. Methods of manufacturing such a reaction vessel are also disclosed.

The present technology provides for a fluorescent detector that is configured to detect light emitted for a probe characteristic of a polynucleotide. The polynucleotide is undergoing amplification in a microfluidic channel with which the detector is in optical communication. The detector is configured to detect minute quantities of polynucleotide, such as would be contained in a microfluidic volume. The detector can also be multiplexed to permit multiple concurrent measurements on multiple polynucleotides concurrently.

The present invention provides devices, systems, and methods for rapid and easy-to-use in sample thermal cycling or temperature changes for the facilitation of reactions such as but not limited to PCR.

The present invention relates to a method for the sensitive identification of high-affinity complexes made of two ligands (2, 3, 4, 5, 6, 7) and one receptor (1). A large number of different ligands (2, 3, 4, 5, 6, 7) of a chemical library are hereby contacted with at least one receptor (1) in a solution. The ligands of the library have a single-strand DNA (8, 9) or RNA with a base length of 2 to 10 bases or alternatively more than 10 bases. In addition, the solution is incubated for a specific period of time and complexes made of two ligands (2, 3, 4, 5, 6, 7) and one receptor (1) are identified.

The present disclosure relates to methods, devices and systems for thermal cycling of a microfluidic cartridge comprising a transparent heat sink and/or a flexible thermal spreader to seal one or more channels on the microfluidic cartridge.

A point-of-care device for detecting nucleic acid is disclosed. The point-of-care device for detecting nucleic acid according to an exemplary embodiment of the present invention includes a rotating body in which a plurality of test tubes containing a sample mixed with heat-generating particles that generate heat when irradiated with light beams are radially coupled around a rotation shaft; a first actuator for rotating the rotating body such that the test tube rotates about the rotation shaft; and an irradiation module for irradiating the light beams to an irradiated area which is set on a rotation path of the test tube, wherein the rotation path includes a non-irradiated area to which the light beams are not irradiated, and wherein the test tube proceeds through the irradiated area and the non-irradiated area on the rotation path according to the rotation of the rotating body.

A collecting apparatus for bacteria includes: a laser beam source configured to emit a laser beam; and a container configured to hold a dispersion liquid in which a plurality of bacteria are dispersed. The container has a bottom surface and an inner side surface. A thin film for converting the laser beam from the laser beam source into heat is formed on the bottom surface. At the inner side surface, immersion wetting occurs by the dispersion liquid when the inner side surface comes into contact with the dispersion liquid. The thin film is configured to produce a thermal convection in the dispersion liquid by heating the dispersion liquid. The inner side surface is configured to produce a Marangoni convection at a gas-liquid interface as an interface between the dispersion liquid and gas around the dispersion liquid.

The present technology provides for an apparatus for detecting polynucleotides in samples, particularly from biological samples. The technology more particularly relates to microfluidic systems that carry out PCR on nucleotides of interest within microfluidic channels, and detect those nucleotides. The apparatus includes a microfluidic cartridge that is configured to accept a plurality of samples, and which can carry out PCR on each sample individually, or a group of, or all of the plurality of samples simultaneously.

The technology described herein generally relates to systems for extracting polynucleotides from multiple samples, particularly from biological samples, and additionally to systems that subsequently amplify and detect the extracted polynucleotides. The technology more particularly relates to microfluidic systems that carry out PCR on multiple samples of nucleotides of interest within microfluidic channels, and detect those nucleotides.

Systems and methods for light based heating of light absorbing sources for modification of nucleic acids through fast thermal cycling of polymerase chain reaction are provided. The system includes a polymeric fluidic device comprising one or more reaction wells. A first light absorbing material is disposed on a first support to define a reaction well and first and second ports are coupled to the reaction wells. The first and second ports are configured to allow input of a fluidic sample into the reaction well. A lyophilized reagent is pre-loaded in the reaction well. A light source is configured to illuminate the first light absorbing material. A first portion of light illuminated onto the first light absorbing material is absorbed into the first light absorbing material and is configured to elevate the temperature of the first light absorbing material to heat the fluidic sample within the reaction well.