The object of the invention is a device for conducting amplification reaction of biological samples with a system for independent control of the temperature of test tubes in a heating assembly comprising a multipart heating slot located in the cooling system housing, characterised in that the heating assembly comprises at least one heating slot (100) comprising a metal heating sleeve (101) wound around with a bifilar winding wire made of enamelled winding wire (102), which is covered with a composite polymer layer (103), wherein a temperature sensor (104) is located on the surface of the winding wire, and at least one heating slot is mounted on the PCB control board (105) located on the cooling system housing (112).

One aspect of the present disclosure relates to a calorimeter for detecting the presence of a target analyte in a fluid sample. The calorimeter can include a support structure, a hermetically-sealed, thermally decoupled central reaction zone associated with the support structure, at least one droplet transport region, and detection electronics. The at least one droplet transport region can be associated with the support structure and configured to merge a reagent droplet with a sample droplet including the fluid sample to form a reaction droplet in the central reaction zone. The detection electronics can be in electrical and/or thermal communication with the central reaction zone and associated with the support structure. The calorimeter can be configured to detect a heat of reaction produced by a reaction event between the target analyte and a capture reagent upon formation of the reaction droplet.

According to one embodiment, an automatic analyzing apparatus includes a liquid tank, a first pump, a dispensing probe, and a thermal exchanger. The liquid tank stores a first liquid. The first pump pressurizes and sends the first liquid supplied from the liquid tank. The dispensing probe uses the first liquid that is sent out from the first pump as a pressure transmitting medium. The thermal exchanger exchanges heat between an atmosphere in the automatic analyzing apparatus and the first liquid in at least a part of a first flow path connecting the first pump to the liquid tank.

A temperature control device for controlling a temperature of a substance to obtain a first temperature and to change to a second temperature has first and second heating blocks and a heating device. The heating device heats the first and second heating blocks to the first and second temperatures, respectively. The temperature control device has a first material opposing the first heating block to define a first space between them arranged to receive the substance and to define a first temperature zone having substantially the first temperature. The temperature control device has a second material opposing the second heating block such as to define a second space between them arranged to receive the substance and define a second temperature zone having substantially the second temperature, the first and second spaces being arranged such that the substance can be moved from the first temperature zone to the second temperature zone.

A system for preparing samples for chemical analysis comprises at least one sample container, and a container receptacle apparatus for receiving the sample container. The sample container comprises an elongate tubular body having a crucible portion proximal to a closed end for receiving a sample therein, and an expansion portion proximal to an open end. The container receptacle apparatus comprising a housing having a heating compartment, a cooling compartment spaced apart from the heating compartment, and an insulating region located between the heating compartment and the cooling compartment. The heating compartment is shaped to receive the crucible portion of the sample container, and the cooling compartment is shaped to receive the expansion portion of the sample container. The apparatus also includes a heating mechanism for heating the sample within the crucible portion of the sample container, and a cooling mechanism for cooling the expansion portion of the sample container.

An insulated chamber having an interior region for storing items therein includes a phase change material to facilitate controlling the temperature of the interior region and the items. A heating device or cooling device may be used to melt or freeze the phase change material. The phase change material (PCM) may be in various locations such as the walls of the chamber in the form of packets or in the form of containers that serve as shelves and may be removable from the interior region. The packets may have recesses for receiving the items. The phase change material may be within capsules that may be within a liquid or a solid matrix. Controls may be provided to control humidity, oxygen, and carbon dioxide within the interior chamber.

A method for the regulated humidification and temperature control of a gas mixture in an inner chamber of an incubator, which inner chamber is sealed off from the surroundings by a housing, includes conducting heat from at least one condensation surface inside the incubator, which at least one condensation surface is separate from the inner surfaces of the inner chamber, is conducted out of the incubator to the outside through a wall delimiting the inner chamber by a heat conductor. Outside the incubator, the heat conductor is connected to a cooling body which releases the heat to the relatively cold ambient air. The method allows dispensing with a cooling unit and at the same time avoid sources of contamination due to moisture condensing out.

A micro-fluidic device includes a plurality of heating elements on a substrate for heating the substrate. They define a plurality of temperature regions having distinct temperatures on the substrate. A flow feature layer is formed above the substrate to define a channel extending across the substrate through each temperature region. As fluid is repeatedly pumped within the channel, it flows from one temperature region to a next temperature region to undergo thermal cycling. Fluid wells reside in the flow feature layer under a cover and connect to the channel whereby fluid can dwell for diagnostic applications.

A heating system for a EWOD device using a single, spatially-structured temperature control element, used to create a zone on the device with a specific temperature profile. The heating system uses multiple contact regions between the temperature control element and the device. One or more of the contact regions are separated from the temperature control element by one or more thermally resistive layers whose purpose is to restrict heat flow from the temperature control element to the device, and further to restrict lateral flow of heat between adjacent contact regions. The heating system can use one or more materials with different thermal resistance to alter the heat flow to different regions of the device. The spatial location of the contact regions is also used to determine the temperature profile within the device. The device has an optional temperature control element which offsets the low temperature point from the inlet temperature. This invention also describes methods to process multiple droplets within the multiple temperature zones.

A nucleic acid analysis apparatus with isothermal based amplification includes a chamber, a fluid delivery unit, a thermal unit, a rotational driven unit and at least one optical unit. The chamber includes a cartridge mounted therein. The fluid delivery unit is connected with the chamber and adapted to transport reagents within the cartridge for sample purification and/or nucleic acid extraction. The thermal unit is disposed in the chamber and adapted to provide a predefined temperature for nucleic acid amplification. The rotational driven unit is connected with the chamber and comprises a motion control unit, wherein the motion control unit is capable of pressing the cartridge during sample purification and/or nucleic acid extraction and rotating the cartridge with a predefined program during nucleic acid amplification and/or detection. The at least one optical unit is disposed on the chamber and includes plural optical components for detection.