A control device for micro-droplet preparation, the control device including a micro-droplet preparation unit, wherein the micro-droplet preparation unit comprises an asymmetric vibrating micro-droplet generation mechanism (1) and a first dynamic positioning assembly (200); the first dynamic positioning assembly (200) is fixedly connected to the asymmetric vibrating micro-droplet generation mechanism (1); the first dynamic positioning assembly (200) is configured to precisely position the asymmetric vibrating micro-droplet generation mechanism (1); and the asymmetric vibrating micro-droplet generation mechanism (1) is configured to generate micro-droplets through asymmetric reciprocating motion. The use of the control device enables the micro-droplets having a uniform size and a controllable volume to be prepared only by regulating a flow rate of a liquid sample and a vibration frequency of a sample injector.