Disclosed is a liquid-sealed cartridge in which a liquid is transferred by a centrifugal force generated when the liquid-sealed cartridge is rotated around a rotation shaft, including: a liquid storage portion configured to store the liquid therein; a seal having an outer peripheral portion connected to the liquid storage portion, the seal being configured to seal the liquid storage portion; a flow path connected to the liquid storage portion via the seal, through which the liquid in the liquid storage portion is transferred by the centrifugal force in a direction away from the rotation shaft, wherein, when the seal receives a pressing force, the seal is inclined in a pressing direction, with one portion of the outer peripheral portion thereof remaining connected with the liquid storage portion, and the other portion of the outer peripheral portion being separated from the liquid storage portion.

The present invention is directed to a cartridge device for a measuring system for measuring viscoelastic characteristics of a sample liquid, in particular a blood sample, comprising a cartridge body having at least one measurement cavity formed therein and having at least one probe element arranged in said at least one measurement cavity for performing a test on said sample liquid; and a cover being attachable on said cartridge body; wherein said cover covers at least partially said at least one measurement cavity and forms a retaining element for retaining said probe element in a predetermined position within said at least one measurement cavity. The invention is directed to a measurement system and a method for measuring viscoelastic characteristics of a sample liquid.

The present invention provides a reciprocal-flow-type nucleic acid amplification device comprising: heaters capable of forming a denaturation temperature zone and an extension/annealing temperature zone; a fluorescence detector capable of detecting movement of a sample solution between the two temperature zones; a pair of liquid delivery mechanisms that allow the sample solution to move between the two temperature zones and that are configured to be open to atmospheric pressure when liquid delivery stops; a substrate on which the chip for nucleic acid amplification according to claim 2 can be placed; and a control mechanism that controls driving of each liquid delivery mechanism by receiving an electrical signal from the fluorescence detector relating to movement of the sample solution from the control mechanism; the device being capable of performing real-time PCR by measuring fluorescence intensity for each thermal cycle.

A microfluidic device can comprise a plurality of interconnected microfluidic elements. A plurality of actuators can be positioned abutting, immediately adjacent to, and/or attached to deformable surfaces of the microfluidic elements. The actuators can be selectively actuated and de-actuated to create directed flows of a fluidic medium in the microfluidic (or nanofluidic) device. Further, the actuators can be selectively actuated and de-actuated to create localized flows of a fluidic medium in the microfluidic device to move reagents and/or micro-objects in the microfluidic device.

Reagent delivery systems, which can include a reagent trough and a pump system, are useful for delivering liquids to a laboratory workbench. Processing samples on the laboratory workbench can result in a large amount of liquid waste. Described herein are reagent troughs, pump systems, reagent delivery systems, waste management systems, and methods of using the same.

Microfluidic channels networks and systems are provided. One network includes a first fluid channel having a first depth dimension; at least a second channel intersecting the first channel at a first intersection; at least a third channel in fluid communication with the first intersection, at least one of the first intersection and the third channel having a depth dimension that is greater than the first depth dimension. Also provided is a flow control system for directing fluids in the network. Systems are additionally provided for flowing disrupted particles into a droplet formation junction, whereby a portion of the disrupted particles or the contents thereof are encapsulated into one or more droplets. Further provided is a method for controlling filling of a microfluidic network by controlling passive valving microfluidic channel network features.

A microdroplet treatment device, comprising: a sample introduction system, a microfluidic chip system (46), a temperature control system, a droplet recognition system, a droplet detection system and a control system (45); the sample introduction system is used for introducing, into the microdroplet treatment device, samples of aqueous phase and oil phase, and the sample introduction system comprises at least: a sample introduction system I (41) and a sample introduction system II (42), the sample introduction system I (41) being used for introducing, into the microdroplet treatment device, samples of oil phase, and the sample introduction system II (42) being used for introducing into the microdroplet treatment device, samples of aqueous phase; the microfluidic chip system (46) comprises a substrate (4), pipes (1a, 1b, 1c, 1d, 2, 3) formed in the substrate, and a first detection window (9) and a second detection window (10); the temperature control system comprises: a temperature sensor and a temperature control member; the droplet recognition system comprises: a laser light source (55) and a photoelectric sensor (56); the droplet detection system comprises: an optical fiber (57), a spectrometer (58) and a halogen light source (59); and the control system (45) is used for controlling each of the systems in the microdroplet treatment device.

A cassette may include a substrate, a die coupled to the substrate, and an electrical interconnection pad layout formed on a first side of the substrate. The electrical interconnection pad layout may include a first row of interconnect pads including at least one interconnect pad. Each interconnect pad of the first row of interconnect pads may be electrically coupled to one of a first set of vias. The electrical interconnection pad layout may also include a second row of interconnection pads including at least one interconnect pad. Each interconnect pad of the second row of interconnect pads being electrically coupled to one of a second set of vias. The second set of vias electrically coupled to the second row of interconnect pads are offset relative to an alignment of the interconnect pads of the first and second rows.

A microfluidic device may include an impedance sensor located within a fluidic priming orifice within a fluidic channel of the microfluidic device, and control logic. The control logic is to force a current into the impedance sensor to sense the presence of a fluid within the fluidic channel at the locations of the impedance sensor, and determine if the fluid is primed into the fluidic channel based on the impedance values sensed by the impedance sensor.

A system and the method of use for processing a cell therapy product includes a centrifugation container and a rotor generating centrifugal field that extends to the centrifugation container. The centrifugation container has a substantially cylindrically shaped container body and a movable plunger rod. The container body contains cell suspension and the movable plunger rod is configured to have a mixing blade at the end of the movable plunger rod contacting the cell suspension in the container body. The mixing blade on the movable plunger rod generates mixing force applied to the cell suspension when the movable plunger rod is rotated around axis of the container body. The end of the movable plunger rod contacting the cell suspension is also movable in the container body along longitudinal direction of the container body when withdrawing and expelling fluid in and out of the centrifugation container body.