A method for diluting, mixing, and/or aliquoting two liquids using a microfluidic system with at least two pump chambers first includes filling at least one of the at least two pump chambers with a first liquid. The at least two pump chambers are connected to one another by at least one microfluidic channel. The method then includes pumping the first liquid through the channel. The at least one channel is configured such that at least part of the first liquid remains in the channel after the pumping. The method then includes determining the part of the first liquid that remains in the channel. The method then includes flushing the channel with a second liquid.

The present disclosure provides a microfluidic chip, including: a support and an NFC coil in the support, and a flow path is provided in the support and is isolated from the NFC coil; the flow path includes at least one detection window region with a stationary phase therein, and the stationary phase is used to specifically capture a target analyte, the detection window region at least partially overlaps with the NFC coil in a thickness direction of the support. The present disclosure further provides a detection method of the microfluidic chip and a micro total analysis system. The present disclosure can quickly and immediately obtain a detection result.

Provided are vertical flow detection devices and related methods. The devices may comprise a membrane having a first surface and a second surface with a plurality of porous structures extending between the first and second surfaces to form fluid conduits from a first fluid chamber formed by the first surface and a second fluid chamber formed by the second fluid surface. A capture agent is immobilized on and/or in the membrane. A rigid porous membrane support mechanically supports the membrane and to provide a relatively uniform flow across the membrane. Various gaskets or holder elements are positioned around an outer edge of the membrane to prevent fluid leakage around the membrane. A fluid pump is configured to force a fluid sample flow in a direction from the first fluid chamber to the second fluid chamber.

An integrated technological platform enabling real-time quantitative multiparameter metabolic profiling, utilizing either or both of extra and intracellular optical sensors, individually or simultaneously. A scalable embedded micropocket array structure, generally fabricated on fused silica substrates, facilitates the integration of multiple, spatially separated extracellular sensors for multiparameter analysis in a container formed with the use of an activation mechanism forming part of a device configured to hold the container during the measurements. The creation of hermetically sealed microchambers is carried out with a pneumatically and/or mechanically and/or electromechanically driven device that is “floating” within the holding device and that is optionally equipped with a vacuum/suction mechanism to hold a component of the container at its surface.

An in-vitro diagnostic device including a pipetting unit and a pipetting method, which allow for a more reproducible and more precise pipetting of liquids, when piercing through a lid of a closed liquid container is required. The pipetting unit is controlled to repeat penetration of the lid of the closed liquid container if the pressure difference between the interior of the liquid container and the surrounding is outside an allowable predefined pressure range.

The invention relates to a method for achieving microfluidic perfusion of a spheroid, said method being implemented in a microfluidic device that comprises a cavity (151) for hydrodynamically trapping said spheroid, said method comprising the following steps: performing a first injection of a gel (20) containing said spheroid into the microfluidic network (10), hydrodynamically trapping said spheroid in the trapping cavity (151) of the microfluidic network, performing a second injection of a fluid that is non-miscible with said gel into said microfluidic network with a view to flushing away gel present in the network, except in the trapping cavity (151), cross-linking the gel (20) present around the spheroid, in the trapping cavity (151), performing a third injection of a culture medium (22) into said microfluidic network with a view to perfusing the spheroid petrified in its gelled environment, and located in the trapping cavity (151).

The present invention is directed to a cartridge device for a measuring system for measuring viscoelastic characteristics of a sample liquid, in particular a blood sample, comprising a cartridge body having at least one measurement cavity formed therein and having at least one probe element arranged in said at least one measurement cavity for performing a test on said sample liquid; and a cover being attachable on said cartridge body; wherein said cover covers at least partially said at least one measurement cavity and forms a retaining element for retaining said probe element in a predetermined position within said at least one measurement cavity. The invention is directed to a measurement system and a method for measuring viscoelastic characteristics of a sample liquid.

In accordance with one embodiment, a processing device includes a heated internal wall and a rotating rod positioned within an interior space formed by the heated internal wall. The rotating rod may be hollow and act as an internal heat exchanger. The processing device also includes a plurality of baffles spaced apart from one another along the rotating rod and extending away from the rotating rod towards the heated internal wall. The plurality of baffles or porous, packed basket that rotates with the rotating rod that also may be configured to provide cooling relative to the heated internal wall. The processing device also includes at least one wiper or roller coupled to an edge of at least one of the plurality of baffles or porous, packed basket, coupled to the rotating rod and that contacts the heated internal wall while rotating together with the rotating rod. In another embodiment, a processing device may be used to adsorb reactive gases into a liquid phase while heat is exchanged.

A method using a chemical analyzer for removing a component of a liquid sample which may interfere with a test performed on a test assay includes the steps of adding the liquid sample to a sample cup, transferring a volume of the liquid sample to a mixing cup containing an IMAC (Immobilized Metal Affinity Chromatography) resin containing porous beads to form a sample/resin solution in the mixing cup, using a pipette of the chemical analyzer to repeatedly aspirate the sample/resin solution into a disposable pipette tip of the pipette and expelling the sample/resin solution from the pipette tip into the mixing cup to achieve a mixed sample/resin solution in the mixing cup, and allowing the mixed sample/resin solution in the mixing cup to rest undisturbed so that the interfering component of the liquid sample adheres to the porous beads and the beads settle to a bottom portion of the mixing cup, resulting in a refined liquid sample devoid of the interfering component and occupying an upper portion of the mixing cup for later dispensing on the test assay.

A lateral flow assay device comprising a conjugate pad for receiving a quantity of fluid, and a membrane comprising a test line for determining whether the fluid comprises a target analyte. The device includes an actuator connected to a moving element comprising a shaft or a wheel. A first magnet is connected to the backing of the membrane. Second and third magnets are connected to the moving element. The actuator moves the moving element between first and second positions. In the first position, the opposite poles of the first and second magnets attract each other and maintain a gap between the membrane and the conjugate pad separate. In the second position, the similar poles of the first and third magnets repel each other and close the gap between membrane and the conjugate pad, allowing the fluid to flow from the conjugate pad into the membrane and the test line.