A microfluidic device for analysing nucleic acids includes a pump unit with a pumping volume, a filter unit for receiving a lysate, and a reaction chamber. The pump unit, the filter unit and the reaction chamber are arranged in the stated order in a pump direction of the pump unit. The microfluidic device is configured to pump an elution medium via the pump unit into the filter unit for elution and subsequently into the reaction chamber for further treatment.

Disclosed is a real-time method for detecting copper and silver in water in parts per billion. Total silver is detected by adding a 2% nitric acid solution to the sample; after ten minutes, adding a buffer solution comprising water, sodium bicarbonate, sodium carbonate and EDTA to the sample; adding an indicator comprising Cadion 2B, EtOH, and Triton X-100 to the sample; after one minute, reading the absorbance of the sample using a spectrophotometer with a target peak of 515 nm; and determining the silver concentration by comparing the absorbance of the sample to the absorbances of known silver standards. Total copper is detected by adding a 2% nitric acid solution to the sample; after ten minutes, adding a buffer/indicator solution to the sample, where the solution comprises water, sodium citrate dihydrate, hydroxal amine hydrochloride and bathocuproine disulfonate; after one minute, reading the absorbance of the sample using a spectrophotometer with a target peak of 480 nm; and determining the copper concentration by comparing the absorbance of the sample to the absorbances of known copper standards. A monitoring device for determining the level of copper or silver in a sample implements the disclosed methods.

Lateral flow devices, methods and kits for performing lateral flow assays are provided.

A microfluidic test device has a body, a first chamber having an outlet provided with a first valve and holding a first buffer having a first buffer volume, a primary reaction chamber, a sample inlet for receiving and feeding a sample having a sample volume, into the microfluidic test device, a first fluid path connecting the outlet of the first chamber and the sample inlet, a second fluid path connecting the sample inlet and the primary reaction chamber, a primary test part having a primary test chamber, a third primary fluid path connecting the primary reaction chamber and the primary test part, a primary valve arranged in the third primary fluid path, a flow driving device configured to move fluid from the primary reaction chamber to the primary test part, and a heating assembly configured to heat a reaction fluid in the primary reaction chamber.

The present invention relates to a device and a method for qualitative and quantitative analysis of heavy metals and more particularly provides a device and a method for qualitative and quantitative analysis of heavy metals utilizing a rotary disc system.

There is provided a sample processing cartridge comprising a. a sample entry location; b. a closed sample processing chamber; c. a sample analysis location comprising a sample analysis well; d. a first channel fluidly connecting the sample entry location and the sample processing chamber; e. a second channel connecting the sample analysis location and the sample processing chamber, the second channel comprising a closed or closable second channel valve;
wherein the sample processing chamber comprises a second channel port providing fluid connection between the second channel and the sample processing chamber, the second channel port being positioned in a sample accumulating region of the sample processing chamber.

There is also provided a sample processing system comprising the cartridge, and methods of use of the cartridge and processing system in a sample processing assay.

A fluidic device for culturing cells includes a microplate and plate lid. The microplate includes multiple wells and channels, the channels extending between the wells such that the channels interconnect the wells. The plate lid releasably engages the microplate to thereby enclose the wells and the channels. The wells include a culture surface such that a cell culture medium received therein is deposited over the culture surface. At least one channel that extends between adjacent ones of the wells is spaced from the culture surfaces of the adjacent wells defining a gap between the at least one channel and the culture surfaces of the adjacent wells for collection of the cell culture medium.

A microfluidic chip for culturing and real-time monitoring of multicellular tissues and use method thereof. The chip comprises a glass substrate layer, and a PDMS microchannel layer located on the glass substrate layer, wherein the glass substrate layer comprises a glass substrate, and a plurality of microelectrodes thereon; the PDMS microchannel comprises a plurality of independent microfluidic channels; the microelectrodes on the glass substrate are in one-to-one correspondence with the microfluidic channels in the PDMS microchannel layer; and the microelectrodes are electrically connected to an external circuit. The use method comprises: cell capture, cell or tissue culture, electrical impedance spectroscopy detection, and tissue release.

A method for designing a microfluidic device includes steps of: a) receiving an input design of a bare microfluidic channel to which one or more cilia layers are to be added, the bare microfluidic channel having a predetermined cross section, the bare microfluidic channel defining an inner surface and an outer surface, a first direction being a fluid flow direction along a length of the bare microfluidic channel and a second direction perpendicular to the first direction; b) receiving operation parameters for a ciliated microfluidic channel formed from the bare microfluidic channel, the operation parameters including fluid viscosity and an opposing pressure gradient in an adverse direction to the fluid flow direction; and c) determining cilia design parameters for the one or more cilia layers to be attached to and distributed over the inner surface, the cilia design parameters being determined from the incompressible Brinkman equation.

Disclosed are microfluidic flow-based electroporation systems that have a flow device, an electrical control module, a fluid delivery module, and a multi-well module. The systems can be used in methods of selecting an electroporation parameter, and in methods of electroporating cells using the selected parameters.