Methods and devices for isolating microbial cells from a sample, extracting eukaryotic DNA from a sample, and identifying the microbial species in the sample are disclosed herein.

A modular fluidic chip includes a body configured to have at least one flow channel formed in an inside thereof and be connected to another modular fluidic chip to allow the at least one flow channel to communicate with a flow channel provided in the other modular fluidic chip. A fluidic chip capable of performing one function is formed in the form of a module, whereby a fluidic flow system of various structures can be implemented without restriction in shape or size by connecting a plurality of fluidic chips capable of performing different functions as necessary. Through this, various and accurate experimental data can be obtained, and when a specific portion is deformed or damaged, only the fluidic chip corresponding thereto can be replaced, thereby reducing manufacture and maintenance costs.

A sample extraction chip and a biological reaction device are disclosed according to the present disclosure. The sample extraction chip includes a chip body and a sample extraction module provided on the chip body, the sample extraction module includes a sample-loading lysis unit, a liquid release-control unit, an extraction unit, a liquid switch-control unit, a liquid collection unit and a sample collection unit, which are connected through flow channels in a sequence of extraction. The liquid release-control unit is configured to store and release liquid reagents, and the liquid switch-control unit is configured to switch between communication of the liquid collection unit and the extraction unit and communication of the sample collection unit and the extraction unit. The sample collection unit includes a front collection portion and a rear collection portion which are both in communication with the liquid switch-control unit.

Examples herein involve amplification and detection of nucleic acids using a microfluidic reaction chamber. An example apparatus includes a reaction-chamber circuit to process a reagent and a biologic sample for amplification of nucleic acids. The apparatus further includes a plurality of capillaries to pass the reagent and the biologic sample through the microfluidic reaction chamber. A valve control system may selectively control each of a plurality of valves to cause the reagent and the biologic sample to selectively move through the microfluidic reaction chamber for the amplification of the nucleic acids according to a particular timing sequence. In various examples, a trapping region disposed in the microfluidic reaction chamber secures the nucleic acids in the microfluidic reaction chamber for amplification using the reaction-chamber circuit

A nucleic acid detection plate comprises a piezoelectric sensor and at least a pipe flowing through the surface of the piezoelectric sensor, two valves intervally installed on the pipe relative to the upstream end of the piezoelectric sensor, the nucleic acid to be detected is blocked in the pipe between the two valves for isothermal amplification; the nucleic acid detection system comprises the nucleic acid detection plate described above, a thermostat capable of accommodating the nucleic acid detection plate; and a signal processor capable of being date connected to the piezoelectric sensor. The inventive method simplifies device structure through coordinated detection by combination of thermostatic amplification and piezoelectric sensing, and improves detection efficiency.

Disclosed herein are media for culture of cells, tissues, and/or organs. The media formulations disclosed herein can be used to support growth, viability, and/or function of one or more than one cell type, tissue, or organ. In some embodiments, one or more cell types, tissues, organ devices, and/or organs are contacted with a disclosed culture medium under conditions sufficient to support growth, viability, and/or function of the cell types, tissues, and/or organs. The disclosed media can be used in methods of culturing multiple cell types, and in some examples, is used in a platform device including one or more organ devices, for example, by circulating the medium through the one or more organ devices in the platform.

Devices and methods for the detection of analytes are disclosed. Devices and methods for detecting food-borne pathogens are disclosed.

A device for analyzing a liquid sample. The device includes an inlet for receiving the sample, a reaction chamber, an analysis module, and at least one pump for moving fluid within the one or more flow paths. The device includes one or more flow paths arranged so as to provide a fluid flow path between the inlet and the reaction chamber, and a fluid flow path between the reaction chamber and the analysis module. The device may be used for analyzing a liquid sample, such as, but not limited to nipple aspirate fluid (NAF).

A fluid dispensing device includes a dispensing cylinder, a first inlet and a second inlet. The first inlet is disposed within a first container on which the fluid dispensing device is mounted. The first inlet and the second inlet are configured to facilitate intake of only one fluid into the dispensing cylinder at a given point in time. The fluid dispensing device also includes a first outlet and a second outlet. The first outlet and the second outlet are configured to dispense only one fluid out of the dispensing cylinder at a given point in time. The fluid dispensing device further includes a valve assembly fluidically coupled to the first inlet and the second inlet, and to the first outlet and the second outlet. The valve assembly is configured to control flow of the fluids within and/or out of the dispensing cylinder.

This disclosure describes single-use test cartridges, cell analyzer apparatus, and methods for automatically performing microscopic cell analysis tasks, such as counting blood cells in biological samples. A small unmeasured quantity of a biological sample such as whole blood is placed in the disposable test cartridge which is then inserted into the cell analyzer. The analyzer isolates a precise volume of the biological sample, mixes it with self-contained reagents and transfers the entire volume to an imaging chamber. The geometry of the imaging chamber is chosen to maintain the uniformity of the mixture, and to prevent cells from crowding or clumping, when it is transferred into the imaging chamber. Images of essentially all of the cellular components within the imaging chamber are analyzed to obtain counts per unit volume. The devices, apparatus and methods described may be used to analyze a small quantity of whole blood to obtain counts per unit volume of red blood cells, white blood cells, including sub-groups of white cells, platelets and measurements related to these bodies.