Devices, systems, and methods for detecting molecules of interest within a collected sample are described herein. In certain embodiments, self-contained sample analysis systems are disclosed, which include a reusable reader component, a disposable cartridge component, and a disposable sample collection component. The reader component may communicate with a remote computing device for the digital transmission of test protocols and test results. In various disclosed embodiments, the systems, components, and methods are configured to identify the presence, absence, and/or quantity of particular nucleic acids, proteins, or other analytes of interest, for example, in order to test for the presence of one or more pathogens or contaminants in a sample.

The invention relates to a urine collection device (100) for collecting midstream urine; wherein said device, in the orientation intended during urine collection, comprising: a first recipient (2) for urine; a second recipient (4) for urine; a urine diverting means (5); wherein said urine collection device (100) comprises a third recipient (14) for urine, wherein said third recipient for urine comprises an evacuated container; or wherein said third recipient (14) for urine comprises a container which is configured for becoming evacuated by being in fluid connection with a vacuum pump; wherein said urine collection device comprises a conduit (16) having an opening (18) in a first end (20) and an opening (22) in a second end (24) thereof; wherein said first end (20) of said conduit (16) is arranged at the interior of said second recipient (4) for urine; wherein said opening (22) in said second end (24) of said conduit (16) is in fluid connection with the interior of said third recipient (14) for urine; wherein said opening of said first end (20) of said conduit 16 comprises a moist soluble plug (26), thereby blocking access through said conduit between said second recipient (4) for urine and said third recipient (14) for urine until dissolution of said plug.

A device for biofluid processing and analysis includes a microfluidic module including multiple stacked layers, each layer of the stacked layers defines a respective conduit, and conduits of the stacked layers are interconnected to provide a flow path for a biofluid.

The present technology provides for an apparatus for detecting polynucleotides in samples, particularly from biological samples. The technology more particularly relates to microfluidic systems that carry out PCR on nucleotides of interest within microfluidic channels, and detect those nucleotides. The apparatus includes a microfluidic cartridge that is configured to accept a plurality of samples, and which can carry out PCR on each sample individually, or a group of, or all of the plurality of samples simultaneously.

A microfluidic chip for conducting microbiological assays, comprises a substrate in which incubation segments, a sample reservoir and microfluidic channels connecting said sample reservoir with said incubation segments are arranged. Said microfluidic chip further comprise a non-aqueous liquid reservoir for containing non-aqueous liquid wherein said reservoir is connectable via a releasable airtight and liquid-tight valve with said microfluidic channels connecting said sample reservoir with said incubation segments each incubation segment comprises an incubation well (113) connected by a gas-exchange channel (115) to an unvented gas cavity (111).

A re-sealable vessel includes a body with an open end and a lid assembly. The lid assembly includes a cap with a seal sized to seal against the open end of the body and the cap is at least partially formed from a fusible metal with a melting point less than a threshold temperature comprising a boiling point or a temperature associated with a threshold vapor pressure of a liquid contained within the body, such that when a temperature of the fusible metal exceeds the melting point, the fusible metal melts, leaving an aperture through the cap.

The present technology provides for an apparatus for detecting polynucleotides in samples, particularly from biological samples. The technology more particularly relates to microfluidic systems that carry out PCR on nucleotides of interest within microfluidic channels, and detect those nucleotides. The apparatus includes a microfluidic cartridge that is configured to accept a plurality of samples, and which can carry out PCR on each sample individually, or a group of, or all of the plurality of samples simultaneously.

A chip includes a first chamber that stores a fluid, a second chamber that stores a mixing target which is to be mixed with the fluid, a flow path that allows a communication between the first chamber and the second chamber, and a valve that is provided in the flow path and capable of changing the flow path by change in shape by heating.

The present technology provides for a microfluidic substrate configured to carry out PCR on a number of polynucleotide-containing samples in parallel. The substrate can be a single-layer substrate in a microfluidic cartridge. Also provided are a method of making a microfluidic cartridge comprising such a substrate. Still further disclosed are a microfluidic valve suitable for use in isolating a PCR chamber in a microfluidic substrate, and a method of making such a valve.

Devices, systems, and methods for detecting molecules of interest within a collected sample are described herein. In certain embodiments, self-contained sample analysis systems are disclosed, which include a reusable reader component, a disposable cartridge component, and a disposable sample collection component. The reader component may communicate with a remote computing device for the digital transmission of test protocols and test results. In various disclosed embodiments, the systems, components, and methods are configured to identify the presence, absence, and/or quantity of particular nucleic acids, proteins, or other analytes of interest, for example, in order to test for the presence of one or more pathogens or contaminants in a sample.