A sample vessel includes a biological sample container and a sample stabilizer container. The biological sample container is configured to receive a biological sample and to store the biological sample. The sample stabilizer container is configured to contain a stabilizer associated with the biological sample. The sample stabilizer container is assembled from a stabilizer vial, an adaptor, and a fluid channel. The stabilizer vial is configured to store an amount of the stabilizer. The adaptor is configured to secure the biological sample container and the stabilizer vial such that the biological sample container and the stabilizer vial form the sample vessel. The fluid channel extends through the adaptor from the stabilizer vial to the biological sample container, the biological sample moving from the biological sample container into the stabilizer vial through the fluid channel.

A cartridge for sample handling and sensing includes (i) a sample port; (ii) a first fluid port connected to the sample reservoir in the distal region via a first fluid channel; and (iii) a second fluid port connected to the sample reservoir via a second fluid channel. The cartridge includes (i) a sensor platform comprising a bulk acoustic wave (BAW) resonator and a fluid flow path comprising a sensing region extending across a sensing surface of the BAW resonator; and (ii) a fluid valve between the sample reservoir and the sensing region. A sample may be applied to the sample port; first volume of fluid may be injected through the first fluid port; and then a second volume of fluid may be injected through the second fluid port to drive the sample into the sensing region of the fluid flow path.

The present invention relates to a microfluidic chip (300) comprising an inlet channel and an output channel in close proximity; systems comprising the same configured to flow a continuous phase without disrupting the integrity of a population of dispersed phase droplets and/or to homogenize a locally static continuous phase throughout droplet loading or generation; and methods using the same.

The present invention relates to a loading well (320) comprising a lateral wall part (3211) in cross-section parallel to the base plan (x/y) and/or a bottom wall part comprising at least one sloped bottom section (32121). The present invention also relates to a microfluidic chip comprising the same; systems comprising the same configured to reduce the dead volume of a drop of sample to be loaded in the microfluidic chip and/or to trap a drop in a defined location; and methods using the same.

Systems and methods are provided for producing isolated microfluidic droplets. In one aspect, a microfluidic system comprises a droplet isolation device and an injection system. The droplet isolation device includes at least one isolation unit and at least one capillary valve. The isolation unit has at least one chamber configured to receive at least two different aqueous solutions without mixing prior to entering the at least one chamber based at least in part on pressure levels of the at least two different aqueous solutions. The injection system includes an aqueous inlet, a non-aqueous inlet, a bypass outlet, a working fluid outlet, and a loading chamber. The injection system is configured to allow for a predetermined amount of each of the at least two different aqueous solutions to be delivered to the droplet isolation device sequentially.

A microfluidic device with a microfluidic circuit including an array of fluidly coupled microchambers. Each microchamber includes a reaction chamber and an associated vent chamber. The microfluidic circuit may be arranged so that a fluid sample introduced to microfluidic device flows into the reaction chamber and air or other gas present in the reaction chamber is vented from the microchamber through the vent chamber. The microchamber may be configured to allow only the flow of air into the vent chamber from the reaction chamber until the air has been displaced from the reaction chamber by the fluid sample and/or a predefined volume of the fluid sample has been received in the reaction chamber. The microchamber may be further configured to release the fluid sample to thereafter flow from the reaction chamber into the vent chamber.

A semiconductor package structure includes a substrate, a die and a conductive structure. The die is disposed on or within the substrate. The die has a first surface facing away from the substrate and includes a sensing region and a pad at the first surface of the die. The first surface of the die has a first edge and a second edge opposite to the first edge. The sensing region is disposed adjacent to the first edge. The pad is disposed away from the first edge. The conductive structure electrically connects the pad and the substrate. The sensing region has a first end distal to the first edge of the first surface of the die. A distance from the first end of the sensing region to a center of the pad is equal to or greater than a distance from the first end of the sensing region to the first edge of the first surface of the die.

The present invention relates to a microfluidic based assay system, comprising a disposable assay cartridge and associated reading device, as well as the individual components themselves. The present invention also relates to methods of conducting assays, using the cartridge and device of the invention, as well as kits for conducting assays.

The invention relates to an assembly for contactless pressure-controlled release of a fluid comprising a non-compressible compartment, at least two fluids in fluidic contact and enclosed inside the non-compressible compartment, one of the two fluids being compressible, and one channel for fluid flow.

Microfluidic devices and methods for investigating crystallization and/or for controlling a reaction or a phase transition are disclosed. In one embodiment, the microfluidic device includes a reservoir layer; a membrane disposed on the reservoir layer; a wetting control layer disposed on the membrane; and a storage layer disposed on the wetting control layer, wherein the wetting control layer and the storage layer define a microfluidic channel comprising an upstream portion, a downstream portion, a first fluid path in communication with the upstream and the downstream portions, and a storage well positioned within the first fluid path, wherein the wetting control layer includes a fluid passageway in communication with the storage well and the membrane, and wherein the wetting control layer wets a first fluid introduced into the microfluidic channel, the first fluid comprising a hydrophilic, lipophilic, fluorophilic or gas phase as the continuous phase in the microfluidic channel.