Assay cartridges are described that have purification, reaction, and detection zones and other fluidic components which can include sample chambers, waste chambers, conduits, vents, reagent chambers, reconstitution chambers and the like. The assay cartridges are used to conduct multiplexed nucleic acid measurements. Also described are kits including such cartridges, methods of using the same, and a reader configured to analyze an assay conducted using an assay cartridge.

Devices and methods for the detection of analytes are disclosed. Devices and methods for detecting food-borne pathogens are disclosed.

A lateral flow diagnostic assay device is defined by a substrate having a top surface that further includes a sample addition zone for receiving a sample, a transport and reaction zone, and a wicking zone. Each of the sample addition zone, reaction and transport zone and wicking zone are disposed on the top surface of the substrate and fluidically interconnected by means that permit lateral capillary flow along at least one fluid flow path from the sample addition zone to the wicking zone. The assay device further includes a capillary vent disposed in relation to the wicking zone, the capillary vent having an overall length and cross sectional area that creates a backpressure so as to control the flow rate of a sample applied to the assay device.

The disclosed embodiments related to an apparatus and methods for biological sample processing enabling isolation and concentration of microbial or pathogenic constituents from the sample. Sample may be obtained directly from a specimen container, such as a vacutainer, and processed directly without risk of user exposure. The disclosed methods and apparatus provide a convenient and inexpensive solution for rapid sample preparation compatible with downstream analysis techniques.

Disclosed are cartridge components, cartridges, systems, and methods for isolating analytes from biological samples. In various aspects, the cartridge components, cartridges, systems, and methods may allow for a rapid procedure that requires a minimal amount of material from complex fluids.

The present technology provides for a microfluidic substrate configured to carry out PCR on a number of polynucleotide-containing samples in parallel. The substrate can be a single-layer substrate in a microfluidic cartridge. Also provided are a method of making a microfluidic cartridge comprising such a substrate. Still further disclosed are a microfluidic valve suitable for use in isolating a PCR chamber in a microfluidic substrate, and a method of making such a valve.

A system that includes a cartridge housing and a hollow transfer module, according to an embodiment is described herein. The cartridge housing further includes at least one sample inlet, a plurality of storage chambers, a plurality of reaction chambers, and a fluidic network. The fluidic network is designed to connect the at least one sample inlet, a portion of the plurality of storage chambers and the portion of the plurality of reaction chambers to a first plurality of ports located on an inner surface of the cartridge housing. The hollow transfer module includes a second plurality of ports along an outer surface of the transfer module that lead to a central chamber within the transfer module. The transfer module is designed to move laterally within the cartridge housing. The lateral movement of the transfer module aligns at least a portion of the first plurality of ports with at least a portion of the second plurality of ports.

The present invention relates to a microfluidic assay system and associated reading device, as well as the individual components themselves. The present invention also relates to methods of conducting assays, using a disposable system and associated reading device, as well as kits for conducting assays.

A fluorescent in-situ hybridization imaging system, including a flow cell to contain a sample to be exposed to fluorescent probes in a reagent; a plurality of reagent reservoirs, each reagent reservoir including a container to hold a liquid reagent; a valve system to control flow from one of a plurality of reagent reservoirs to the flow cell; a pressure source coupled to each of the plurality of reagent reservoirs to apply a positive pressure to liquid reagent in the container and urge the liquid reagent to flow toward the flow cell; and a fluorescence microscope including a variable frequency excitation light source and a camera positioned to receive fluorescently emitted light from the sample.

The present invention discloses a micro-fluidic chip, comprising a chip main body, a sample inlet, an air inlet, a liquid driving force inlet, an air sublet, a main fluid channel and multiple functional chambers provided on the chip main body; the main fluid channel communicates with the multiple functional chambers, and the liquid driving force inlet is used to connect a liquid driving device capable of driving liquid to flow in the main fluid channel and the multiple functional chambers; the micro-fluidic chip of the present invention identifies, positions and quantifies a liquid sample by means of a specific liquid quantification chamber, thereby decreasing chip manufacturing process difficulty, and increasing quantification accuracy, and is particularly suitable for quantification and testing of whole blood samples.