A buffer management system includes a supply of concentrated buffer solution, an inline dilution skid having a manifold in fluid communication with the supply of concentrated buffer solution and a control unit configured to operate the dilution skid to selectively dilute the supply of concentrated buffer solution in the manifold with a source of water for injection (WFI), and a biocontainer assembly. The biocontainer assembly includes a biocontainer bag defining a storage volume and a fill level sensor configured to generate a fill level signal indicative of the amount of material within the storage volume of the biocontainer bag. The biocontainer bag is in fluid communication with the manifold of the dilution skid for storing diluted buffer solution therein. The fill level sensor communicates the fill level signal to the control unit of the dilution skis, which uses it to manage the operation of the dilution skid.

Described herein are microfluidic devices and methods that can separate and concentrate particles in a sample.

A microfluidic device includes a particle sorting region having a first, second and third microfluidic channels, a first array of islands separating the first microfluidic channel from the second microfluidic channel, and a second array of islands separating the first microfluidic channel from the third microfluidic channel, in which the island arrays and the microfluidic channels are arranged so that a first fluid is extracted from the first microfluidic channel into the second microfluidic channel and a second fluid is extracted from the third microfluidic channel into the first microfluidic channel, and so that particles are transferred from the first fluid sample into the second fluid sample within the first microfluidic channel.

A microfluidic device includes a microchannel having an interior bounded by a side wall, an inlet, a switching region, and a plurality of outlet channels downstream of the switching region. The microchannel is formed in a microfluidic chip substrate and configured to accommodate a flow of liquid through the microchannel. The microfluidic device includes a valve operatively coupled to the switching region comprising a sealed reservoir. A side passage extends between the reservoir and the interior of the microchannel via an aperture in the side wall and is configured to accommodate a volume of liquid between the interior of the microchannel and the reservoir. The microfluidic device includes an actuator integrated into the microfluidic chip and configured to increase an internal pressure of the reservoir and move at least a portion of the volume of the liquid from the side passage into the microchannel to deflect a portion of the liquid flowing through the microchannel.

Methods and systems are for determining the concentration of a chemical species in an analyte solution. At least one train of segments are injected into a microfluidic channel having a first end and a second end, each train of segments having segments of analyte solution and segments of sensing solution which are immiscible with the segments of analyte solution. The train of segments is circulated from the first end to the second end of the microfluidic channel such that a reversible chemical exchange is established between the chemical species of each segment of analyte solution and a chemical indicator of the at least one contacting segment of sensing solution. The response of the chemical indicator is measured at the second end of the microfluidic channel and the concentration of the chemical species in the analyte solution is determined based on the response.

Methods and apparatus for detecting, quantifying, enriching, and/or separating bacterial species in fluid sample are provided. The fluid sample is provided as input to a microfluidic passage of a microfluidic device, wherein the microfluidic device comprises at least one electrode disposed adjacent to the microfluidic passage. The at least one electrode is activated to capture bacteria in the sample using dielectrophoresis, wherein the capture efficiency of bacteria is at least 99%.

Methods and apparatus for detecting, quantifying, enriching, and/or separating bacterial species in fluid sample are provided. The fluid sample is provided as input to a microfluidic passage of a microfluidic device, wherein the microfluidic device comprises at least one electrode disposed adjacent to the microfluidic passage. The at least one electrode is activated to capture bacteria in the sample using dielectrophoresis, wherein the capture efficiency of bacteria is at least 99%.

All interface component (40), suitable for cooperating with a microfluidic device (1), the interface component comprising, one or more elements (41) which can be selectively connected to a pneumatic system (71 a,71 0) which can provide a positive and/or negative air flow to the one or more elements (41); wherein each of the one or more elements 141) comprises, an input port (42) which can be selectively fluidly connected to a pneumatic system (71 a,71 1)); and a flow restrictor (43) according to a further aspect of the present invention; the flow restrictor (43) being arranged in fluid communication with the input port (42), wherein the flow restrictor (43) can restrict the flow of fluid through the element (41); and an aerosol filter (49) which is arranged to be in fluid communication with the flow restrictor (43); and wherein the interface component (40) further comprises one or more outlets (45), each of the one or more outlets (45) being in fluid communication with a respective element (41), so that fluid can flow from the element (41) out of the interface component (40) via the one or more outlets (45); and wherein each of the one or more outlets (45) can be selectively arranged to be in fluid communication with a respective reservoir (105,1(16,107,108) of a microfluidic device (1). There is further provided a corresponding method and assembly for extracting ferromagnetic, paramagnetic and/or diamagnetic particles from a sample.

Described herein are microfluidic devices and methods that can separate and concentrate particles in a sample.

The present disclosure provides apparatuses, systems, and methods for performing particle analysis through flow cytometry at comparatively high event rates and for gathering high resolution images of particles.