Disclosed herein is a system to detect and characterize individual particles and cells using at least either optic or electric detection as the particle or cell flows through a microfluidic channel. The system also provides for sorting particles and cells or isolating individual particles and cells.

Methods and systems for manipulating drops in microfluidic channels are provided.

A device is disclosed that comprises a base having first and second reservoirs, each having an inlet and an outlet, and a channel layer comprising an inlet channel in fluid communication with the inlets of the reservoirs, one or more outlet channels in fluid communication with the outlets of the reservoirs, and a channel network comprising at least one channel extending therebetween. In a forward tilted position, a first fluid circuit is formed from the outlet of the first reservoir, through the one or more outlet channels, through the channel network, through the inlet channel, to the both the inlet and outlet of the second reservoir. In a reverse tilted position a second fluid circuit is formed from the outlet of the second reservoir, through the one or more outlet channels, through the channel network, through the inlet channel, to both the inlet and outlet of the first reservoir. Methods of using the device are also disclosed.

Provided herein are compositions, systems, and methods for high throughput screening. In particular, provided herein are microfluidic devices for high throughput analysis of multiplex chemical (e.g., drug interactions) across a wide range of concentrations.

Various systems, methods, and devices are provided for focusing particles suspended within a moving fluid into one or more localized stream lines. The system can include a substrate and at least one channel provided on the substrate having an inlet and an outlet. The system can further include a fluid moving along the channel in a laminar flow having suspended particles and a pumping element driving the laminar flow of the fluid. The fluid, the channel, and the pumping element can be configured to cause inertial forces to act on the particles and to focus the particles into one or more stream lines.

Some embodiments of a micro-fluidic device include at least one inlet hole located on an inlet side of the microfluidic device, the inlet hole consisting of a plurality of holes with diameters smaller in size than a diameter of the at least one inlet hole, at least one outlet hole located on an outlet side of the microfluidic device opposite the inlet side; and a micro-channel, where the plurality of holes are connected to the micro-channel

We describe a method comprising: providing a droplet comprising a plurality of constituents, splitting said droplet into a first droplet and a second droplet, wherein said first droplet comprises a first fraction of said plurality of constituents and said second droplet comprises a second fraction of said plurality of constituents, analysing said constituents of said first fraction of said plurality of constituents in said first droplet, and sorting said second droplet dependent on an outcome of said analysis.

In accordance with some embodiments, a fluid sample collection and retrieval apparatus including a microfluidic chip, a retrieval tube, a first switch, a second switch and a processor is provided. The microfluidic chip includes a first sample channel, a first fluid directing channel assembly, a first confluence chamber, a first collection channel, a first waste channel, and a retrieval hole. The retrieval hole passes through an outer surface of the microfluidic chip. The retrieval tube is connected to the retrieval hole. The first switch is connected to the microfluidic chip. The second switch is attached to the retrieval tube. The processor is configured to activate the first switch to operate the flow adjustment of the first fluid directing channel assembly and activate the second switch to operate a sample collection in the first collection channel within duration of operating the flow adjustment of the first fluid directing channel assembly.

The present disclosure relates to analytical testing devices comprising microfluidics and methods for performing an assay on a fluid sample received within the microfluidics, and in particular, to mitigating drift of fluid samples over a sensor by incorporating a bypass channel into the microfluidics. For example, a test cartridge device is provided that includes a fluid sample entry port and holding chamber connected to a bifurcation junction of a sensor channel and a bypass channel. The sensor channel includes an upstream region and a downstream region, and an analyte sensor is in the upstream region. As a cross-sectional area of the bypass channel is greater than the cross-sectional area of the downstream region of the sensor channel, the bypass channel is a preferred path for excess sample flow and pressure, and thus sample drift above the analyte sensor is mitigated.

A device for the simultaneous filling of microtiter plate wells via centrifugal forces. In an implementation, the device includes a planar attachment for a microtiter plate having one or more liquid reservoirs for filling one well each of the microtiter plate per liquid reservoir. Each liquid reservoir has an upper filling opening for receiving a filling liquid, one or more lower filling nozzles for dispensing the filling liquid into the well of the microtiter plate, and a reservoir wall which connects the filling opening and the filling nozzle to one another. The filling nozzle is arranged off-center to the axis of symmetry of the microtiter plate well and off-center to the axis of symmetry of the filling opening of the filling device in a subregion of the reservoir wall.