Arrays and substrates comprising a material, in particular capture agents and/or detectable targets, attached to the substrates along substantially parallel lines forming a barcoded pattern and related methods and systems.

The invention relates to an apparatus for transporting a fluid in a channel leg of a microfluidic element, especially of a flow cell. According to the invention, a pressure source for pressurizing a front end face (42) in transport direction of the liquid which completely fills the channel leg in cross section is provided. The pressure source preferably comprises a closed space (17; 22; 34; 36, 38, 40), in which a compressed gas, for example air, is compressible by moving the front end face (42) of the fluid transported in the channel leg.

Microfluidic chips and cartridges and systems that include such chips are disclosed. In some embodiments, the chips include a microfluidic channel disposed in a substrate with the channel comprising at least one expansion region. The channel is configured to generate a vortex within the at least one expansion region in response to fluid through the microfluidic channel to trap cells or particles. The substrate in which the channel is formed may be relatively rigid to resist deformation.

Various systems, methods, and devices are provided for focusing particles suspended within a moving fluid into one or more localized stream lines. The system can include a substrate and at least one channel provided on the substrate having an inlet and an outlet. The system can further include a fluid moving along the channel in a laminar flow having suspended particles and a pumping element driving the laminar flow of the fluid. The fluid, the channel, and the pumping element can be configured to cause inertial forces to act on the particles and to focus the particles into one or more stream lines.

The present disclosure is to provide a method of mounting a membrane filter and a microfluidic device including the membrane filter, which are capable of increasing a flow pressure of a fluid. A salt matrix is attached to a region of a surface of the membrane filter that adjoins the filter support structure and is exposed toward the movement passage. A membrane filter and a microfluidic device including the membrane filter, which are capable of increasing a flow pressure of a fluid.

Disclosed are an improved guide device capable of being easily replaced for damage, and a detector having the same.

The guide device includes a first plate configured to have a plurality of grooves disposed in one surface thereof; and a second plate configured to be in contact with the first plate, wherein the second plate is in contact with the first plate to separate a plurality of channels, wherein the first plate is configured so that the plurality of microdroplets pass through any one of the plurality of channels, the fluid passes through channels facing each other among the plurality of flow channels, and the microdroplets are regularly spaced apart by the fluid that is discharged from the channels facing each other.

The present inventive concept relates to a microfluidic system for compensation of evaporation of liquid from channels. The microfluidic system comprises: a compensating microfluidic channel having a first end arranged for hindering capillary driven flow of a liquid out from the compensation microfluidic channel via the first end, and, a second end, being connected to a first microfluidic channel: a sample manipulation portion comprising a plurality of outlet channels, wherein each outlet channel ends in a respective stop valve, wherein the first microfluidic channel connects to the sample manipulation portion, thereby being in fluidic connection with the plurality of outlet channels, wherein each outlet channel of the plurality of outlet channels is arranged to exert a retention capillary pressure on the liquid, wherein the compensating microfluidic channel is arranged to exert a retention capillary pressure on the liquid, wherein the retention capillary pressure of each outlet channel is larger than the retention capillary pressure of the compensating microfluidic channel, such that the liquid flows from the compensating microfluidic channel towards the sample manipulation portion if liquid evaporates from one or more of the plurality of outlet channels at the respective stop valve, thereby compensating for evaporation of the liquid from the plurality of outlet channels at the respective stop valve.

A fluidics module has first fluidics structures having a first fluid chamber, second fluidics structures having a second fluid chamber, a first connection having a fluidic resistance between the first fluid chamber and the second fluid chamber, third fluidics structures having a third fluid chamber, a second connection having a barrier between the first fluid chamber and the third fluid chamber, a pressure compensation channel between the second fluid chamber and the third fluid chamber, and fourth fluidics structures having a fourth fluid chamber connected to the second fluidics structures via a third connection. Liquid can be transferred centrifugally from the first fluid chamber into the second fluid chamber. Liquid from the second fluid chamber can be transferred under rotation into the fourth fluid chamber via the third connection to generate negative pressure in the third fluid chamber. The barrier can be overcome by liquid from the first fluid chamber due to the negative pressure generated to transfer liquid from the first fluid chamber into the third fluid chamber via the second connection.

A microfluidic device includes a first channel, second channels, and a transition channel splitting the first channel into the second channels. The transition has a first end fluidically connected to the first channel and a second end fluidically connected to the second channels. The transition channel expands in width from a width of the first channel at the first end to no less than a sum of widths of the second channels at the second end so as to promote fluid flow from the first channel to the second channels.

A microfluidic device is described. The device comprises a flow channel; and at least one reaction chamber. The at least one reaction chamber comprises a chamber inlet connecting the at least one reaction chamber to the flow channel, a first region adjacent the chamber inlet, a second region spaced from the chamber inlet by the first region, a vent channel and a reaction reagent disposed on at least one inner surface of the second region. Also described is a PCR apparatus and a method of performing PCR.