A magnetic separation device has a membrane having a plurality of pores, a magnetically soft material layer disposed on the membrane, and a passivation layer disposed on the magnetically soft material layer. The magnetic separation device may be part of a microfluidic device having a lateral flow channel and a vertical flow magnetic separation filter. The magnetic separation device may be used to separate magnetically tagged particles, such as cells.

A method for producing a population of T cells that express a chimeric antigen receptor (CAR) comprising the steps of providing a fluid sample including a population of T cells; labeling the population of T cells with magnetic beads; extracting the population of T cells from the fluid sample by flowing the fluid sample through a first conduit that passes through a first magnetic separator device; transducing the population of T cells to express a chimeric antigen receptor (CAR) by contacting the population of T cells with a population of lentivirus in a solution contained in an incubation container; and harvesting the population of T cells by flowing the solution through a second conduit that passes through a second magnetic separator device, wherein the first conduit, the incubation container, and the second conduit are fluidically connected by a network of fluidic lines.

Disclosed herein are devices, systems, and methods of use thereof for magnetic separation. For example, disclosed herein is a device for separation of a mixture comprising a magnetic solid and a non-magnetic solid. The device comprises a separation chamber extending from a proximal end to a distal end; the proximal end defining an inlet; the distal end defining a first outlet and a second outlet. The separation chamber having a region that is configured to subject the fluid flowing through said region to a magnetic field to thereby separate the mixture into a first portion and a second portion; the first portion being enriched with the non-magnetic solid relative to the second separated portion; and the second portion being enriched with the magnetic solid relative to the first separated portion.

Disclosed herein are devices, systems, and methods of use thereof for magnetic separation. For example, disclosed herein is a device for separation of a mixture comprising a magnetic solid and a non-magnetic solid. The device comprises a separation chamber extending from a proximal end to a distal end; the proximal end defining an inlet; the distal end defining a first outlet and a second outlet. The separation chamber having a region that is configured to subject the fluid flowing through said region to a magnetic field to thereby separate the mixture into a first portion and a second portion; the first portion being enriched with the non-magnetic solid relative to the second separated portion; and the second portion being enriched with the magnetic solid relative to the first separated portion.

Provided herein are compositions comprising substrates that contain polymeric materials or non-magnetic, paramagnetic, or diamagnetic metal objects, and films or inks that contain ferromagnetic materials in which the films or the ferromagnetic materials are transparent. Also provided herein are methods of fabricating the substrates. Further provided herein are ferromagnetic material films containing transparent or translucent films that comprises ferromagnetic materials. The coating imparts functionality to the film such that the film is capable of being mechanically separated from the polymeric materials or non-magnetic, paramagnetic, or diamagnetic metal objects using a commercial magnetic separator. The transparent, food-safe ink composition, which can be printed using high-speed flexographic, gravure, intaglio, offset printing or pad printing consists of an ingestible magnetically susceptible pigment capable of rendering the printed template with magnetically active properties.