A method and apparatus for separation of cells from suspension using single use components that include a flexible membrane mounted on a rigid frame that is supported within a multiple use rigid centrifuge bowl. Such single use components include a rotatable core which is rotated at a sufficiently high angular velocity to separate cell concentrate and centrate. Cell centrate and/or cell concentrate is removed from the interior of the core by a centripetal pump having a pair of curved volute passages. The volute passages are curved toward the direction of rotation of the core.

A method and apparatus for separation of cells from suspension using single use components that include a flexible membrane mounted on a rigid frame that is supported within a multiple use rigid centrifuge bowl. Such single use components include a rotatable core which is rotated at a sufficiently high angular velocity to separate cell concentrate and centrate. Cell centrate and/or cell concentrate is removed from the interior of the core by a centripetal pump having a pair of curved volute passages. The volute passages are curved toward the direction of rotation of the core.

A method and apparatus for separating cell suspension into centrate and concentrate includes a solid wall rotatable centrifuge bowl (82). A single use rotatable core (88) is positioned in the bowl and bounds a cavity (90) which serves as a separation chamber. The bowl rotates about an axis (84). An inlet tube (92) and an outlet tube (102) extend along the axis in the bowl cavity. The centripetal pump (98) in the cavity is in connection with the outlet tube. The centrate discharge pump (120) is in connection with the outlet tube. A pressure damping reservoir (122) is fluidly connected between the outlet tube and the discharge pump. At least one control circuit (142) is operative to control the apparatus to maintain a positive pressure above atmospheric pressure within the cavity, so as to maintain the cell suspension away from at least one seal (106) that bounds the interior of the cavity.

A method and apparatus for separating cell suspension into centrate and concentrate includes a solid wall rotatable centrifuge bowl (82). A single use rotatable core (88) is positioned in the bowl and bounds a cavity (90) which serves as a separation chamber. The bowl rotates about an axis (84). An inlet tube (92) and an outlet tube (102) extend along the axis in the bowl cavity. The centripetal pump (98) in the cavity is in connection with the outlet tube. The centrate discharge pump (120) is in connection with the outlet tube. A pressure damping reservoir (122) is fluidly connected between the outlet tube and the discharge pump. At least one control circuit (142) is operative to control the apparatus to maintain a positive pressure above atmospheric pressure within the cavity, so as to maintain the cell suspension away from at least one seal (106) that bounds the interior of the cavity.

Provided is a centrifuge media separator for separating blast particulate from fine particulate carried by air flowing from a blast cabinet and through the media separator. The centrifuge media separator comprises an upper panel, a lower panel, and an outer wall. The upper panel has a central opening formed therein. The outer wall is configured in a truncated logarithmic shape and which extends between the upper and lower panels. The outer wall has at least one particulate escape aperture formed therein. The upper panel, lower panel and outer wall collectively define a curvilinear air passageway having an inlet and an outlet. An air foil extends from the outer wall in to the air passageway. The distance than the air foil extends in to the air passageway is adjustable. The inlet is configured to allow a flow of air to enter the air passageway and circulate therethrough toward the outlet. The escape aperture is configured to exhaust the blast particulate out of the passageway. The central opening is configured to exhaust the fine particulate out of the passageway.

Provided is a centrifuge media separator for separating blast particulate from fine particulate carried by air flowing from a blast cabinet and through the media separator. The centrifuge media separator comprises an upper panel, a lower panel, and an outer wall. The upper panel has a central opening formed therein. The outer wall is configured in a truncated logarithmic shape and which extends between the upper and lower panels. The outer wall has at least one particulate escape aperture formed therein. The upper panel, lower panel and outer wall collectively define a curvilinear air passageway having an inlet and an outlet. An air foil extends from the outer wall in to the air passageway. The distance than the air foil extends in to the air passageway is adjustable. The inlet is configured to allow a flow of air to enter the air passageway and circulate therethrough toward the outlet. The escape aperture is configured to exhaust the blast particulate out of the passageway. The central opening is configured to exhaust the fine particulate out of the passageway.

A modular centrifugal separator system includes a base unit, an exchangeable separation insert arranged therein, an exchangeable tube kit, and an interface for fluid communication between the exchangeable separation insert and the exchangeable tube kit. The exchangeable separation insert includes a rotor casing forming a separation space, and a first half of the interface. The exchangeable tube kit includes at least one tube for one of the liquid feed mixture, the heavy phase, and the light phase, and a second half of the interface. The modular centrifugal separator system includes a removable sealing member covering the first and/or second half of the interface.

A modular centrifugal separator system includes a base unit, an exchangeable separation insert arranged therein, an exchangeable tube kit, and an interface for fluid communication between the exchangeable separation insert and the exchangeable tube kit. The exchangeable separation insert includes a rotor casing forming a separation space, and a first half of the interface. The exchangeable tube kit includes at least one tube for one of the liquid feed mixture, the heavy phase, and the light phase, and a second half of the interface. The modular centrifugal separator system includes a removable sealing member covering the first and/or second half of the interface.

Provided is a chamber configuration for a reverse flow centrifuge, and a reverse flow centrifuge system configured for low fluid volume and small radius rotation. The compact reverse flow centrifuge system has a reusable subsystem and a single use replaceable subsystem. The replaceable subsystem comprises a separation chamber, fluid delivery manifold and rotational mounting connecting the separation chamber to the fluid manifold. The single use replaceable subsystem provides a closed environment for execution of reverse flow centrifugation processes. The separation chamber has a substantially conical fluid enclosure portion connected to a neck portion, and a dip tube extends centrally through the conical fluid enclosure to provide a fluid path to the tip of the conical fluid enclosure.

Provided is a chamber configuration for a reverse flow centrifuge, and a reverse flow centrifuge system configured for low fluid volume and small radius rotation. The compact reverse flow centrifuge system has a reusable subsystem and a single use replaceable subsystem. The replaceable subsystem comprises a separation chamber, fluid delivery manifold and rotational mounting connecting the separation chamber to the fluid manifold. The single use replaceable subsystem provides a closed environment for execution of reverse flow centrifugation processes. The separation chamber has a substantially conical fluid enclosure portion connected to a neck portion, and a dip tube extends centrally through the conical fluid enclosure to provide a fluid path to the tip of the conical fluid enclosure.