Disclosed is a restoration material, a restoration method for abandoned ion-absorbed rare earth tailings area and use thereof, which belongs to the technical field of ecological restoration. The restore material for the abandoned ion-absorbed rare earth tailings area provided by the present disclosure comprises AM fungi and pioneer plants; the species of AM fungi is selected from one or more of G. intraradices, G. mosseae and P. occultum; the pioneer plants is selected from one or more of paspalum, ramie and awn.

A method of inoculating a tree with an ectomycorrhizal fungus is disclosed. The method comprises contacting at least one non-terrestrial adventitious root of the tree with the ectomycorrhizal fungus under conditions suitable for inoculation of the at least one non-terrestrial adventitious root by the ectomycorrhizal fungus. Methods of cultivating the ectomycorrhizal fungus are also disclosed as well as systems and devices for same.

A method of inoculating a tree with an ectomycorrhizal fungus is disclosed. The method comprises contacting at least one non-terrestrial adventitious root of the tree with the ectomycorrhizal fungus under conditions suitable for inoculation of the at least one non-terrestrial adventitious root by the ectomycorrhizal fungus. Methods of cultivating the ectomycorrhizal fungus are also disclosed as well as systems and devices for same.

A planting method for morels is disclosed, including the following steps of: (1) preparing spawn; (2) flipping the spawn; (3) managing the humidity; and (4) fruiting for harvesting. The planting method is simple, is easy to learn and promote, has a high yield, and requires few spawn, thereby reducing planting costs and product costs.

A planting method for morels is disclosed, including the following steps of: (1) preparing spawn; (2) flipping the spawn; (3) managing the humidity; and (4) fruiting for harvesting. The planting method is simple, is easy to learn and promote, has a high yield, and requires few spawn, thereby reducing planting costs and product costs.

A monokaryotic mycelium sheet producing system for creating a sheet of monokaryotic mycelial material. The mycelium sheet producing system includes a culture unit, a spore stock unit, a plating unit, a section unit, a sub-plating unit, an expanding unit and a colonization unit. The culture unit prepares a monokaryon culture. The spore stock unit grows a plurality of fruit bodies in sterile laboratory conditions to create a spore stock. The plating unit performs a peroxide-based spore rescue and a plating process. The section unit is adaptable to section robust hyphae. The sub-plating unit sub-plates and expands the robust hyphae onto a spawn grain master. The expanding unit subsequently expands the spawn grain master into appropriate production of spawn volume. The colonization unit is adaptable to perform a subsequent colonization of mycelium substrate thereby creating a substantially defect free sheet of mycelium.

A monokaryotic mycelium sheet producing system for creating a sheet of monokaryotic mycelial material. The mycelium sheet producing system includes a culture unit, a spore stock unit, a plating unit, a section unit, a sub-plating unit, an expanding unit and a colonization unit. The culture unit prepares a monokaryon culture. The spore stock unit grows a plurality of fruit bodies in sterile laboratory conditions to create a spore stock. The plating unit performs a peroxide-based spore rescue and a plating process. The section unit is adaptable to section robust hyphae. The sub-plating unit sub-plates and expands the robust hyphae onto a spawn grain master. The expanding unit subsequently expands the spawn grain master into appropriate production of spawn volume. The colonization unit is adaptable to perform a subsequent colonization of mycelium substrate thereby creating a substantially defect free sheet of mycelium.

A process of making a mycological biopolymer of a mycomaterial filter comprising the steps of filling a scaffold with a nutritive substrate and a fungus, placing an encasement on the scaffold to seal the scaffold, said encasement having only one outlet therein open to fresh air and defining a vacant space, incubation of the sealed scaffold at high temperatures and carbon dioxide concentrations to induce biopolymer growth into the vacant space wherein the mycological biopolymer environmental conditions comprise an environmental temperature from 55° F. to 95° F. and carbon dioxide constitutes from 2% to 8% of the environment within the vacant space, and thereafter drying the produced mycological biopolymer.

A process of making a mycological biopolymer of a mycomaterial filter comprising the steps of filling a scaffold with a nutritive substrate and a fungus, placing an encasement on the scaffold to seal the scaffold, said encasement having only one outlet therein open to fresh air and defining a vacant space, incubation of the sealed scaffold at high temperatures and carbon dioxide concentrations to induce biopolymer growth into the vacant space wherein the mycological biopolymer environmental conditions comprise an environmental temperature from 55° F. to 95° F. and carbon dioxide constitutes from 2% to 8% of the environment within the vacant space, and thereafter drying the produced mycological biopolymer.

The process of making a biocomposite material utilize a bacterial species and a fungal species in an agricultural feedstock composed of a substrate of non-nutrient discrete particles and a nutrient material wherein the bacterial species imparts mechanical properties to the biocomposite material and the fungal species binds the biocomposite material. Both bacterium and fungus can be genetically engineered to produce desired properties within the microbial communities.