An apparatus for depositing a particulate material onto mushroom compost has a distributor box configured to receive the particulate material, such as mushroom casing, and a moving belt that is fed from a roller into the bottom of the box and exits from a discharge opening of the distributor box. A net pulley roller associated with the moving belt is pulled to advance the moving belt. A ribbed roller mounted for rotation at or near the discharge opening of the distributor box scrapes the particulate material out of the distributor box and deposits the particulate material onto the top surface of the moving belt. The particulate material then rides on the top surface of the moving belt until it drops off the belt onto the mushroom compost at the belt reversal point.

Several types of non-agricultural lignocellulosic waste media are disclosed for the growth of mycological biopolymers. The growth medium is comprised of a substrate with a composition of appropriate Carbon, Nitrogen and mineral components including but not limited to lipids, proteins, and other inherent nutrition requisite for mycelial growth. Specific examples are (1) a lignocellulosic material, (2) a mineral based material, (3) a non-toxic, organic or inorganic, non-lignocellulosic material, (4) a synthetically sourced and produced material, (5) a whole tree (flourized), and (6) an agar media.

Provided is a cultivating material composition including a cultivating substrate and a bio-cellulose film, wherein the bio-cellulose film is in contact with the cultivating substrate, such that highly increased yield and quality of crops, a shorter harvest period, less water and nutrient sources, and low cost can be achieved.

Provided is a cultivating material composition including a cultivating substrate and a bio-cellulose film, wherein the bio-cellulose film is in contact with the cultivating substrate, such that highly increased yield and quality of crops, a shorter harvest period, less water and nutrient sources, and low cost can be achieved.

Disclosed are methods of producing nutrient solutions having unique isotopic fingerprints; methods of producing traceable plants; and methods of identifying the source of a traceable plant that does not rely on expensive artificially separated isotopes. Plants grown with these nutrient solutions will have unique isotopic fingerprints that will be difficult or impossible to counterfeit.

Disclosed are methods of producing nutrient solutions having unique isotopic fingerprints; methods of producing traceable plants; and methods of identifying the source of a traceable plant that does not rely on expensive artificially separated isotopes. Plants grown with these nutrient solutions will have unique isotopic fingerprints that will be difficult or impossible to counterfeit.

A monokaryotic mycelium sheet producing system for creating a sheet of monokaryotic mycelial material. The mycelium sheet producing system includes a culture unit, a spore stock unit, a plating unit, a section unit, a sub-plating unit, an expanding unit and a colonization unit. The culture unit prepares a monokaryon culture. The spore stock unit grows a plurality of fruit bodies in sterile laboratory conditions to create a spore stock. The plating unit performs a peroxide-based spore rescue and a plating process. The section unit is adaptable to section robust hyphae. The sub-plating unit sub-plates and expands the robust hyphae onto a spawn grain master. The expanding unit subsequently expands the spawn grain master into appropriate production of spawn volume. The colonization unit is adaptable to perform a subsequent colonization of mycelium substrate thereby creating a substantially defect free sheet of mycelium.

A mycelium growth bed for optimal production of pure mycelium or a pure mycelium composite with controlled or predictable properties, the bed comprising a tray, a conveying platform, a permeable membrane, a substrate, a porous material and a lid. The permeable membrane is positioned on the conveying platform within the tray. The substrate is positioned on the permeable membrane and the porous material is positioned on top of the substrate. The system provides a configuration having a substrate weight to surrounding space volume ratio between 0.5 and 5.0 g/cc, an air volume (surrounding space) to substrate volume between 0.01 and 1.0, and an air volume (surrounding space) to substrate area is between 0.5 and 5 cc/cm, wherein the CO.sub.2 concentration is held above 3%, the relative humidity is held above 40% and the O.sub.2 concentration is held below 20% in steady state conditions to produce leather-like mycelium without fruiting bodies.

A mycelium growth bed for optimal production of pure mycelium or a pure mycelium composite with controlled or predictable properties, the bed comprising a tray, a conveying platform, a permeable membrane, a substrate, a porous material and a lid. The permeable membrane is positioned on the conveying platform within the tray. The substrate is positioned on the permeable membrane and the porous material is positioned on top of the substrate. The system provides a configuration having a substrate weight to surrounding space volume ratio between 0.5 and 5.0 g/cc, an air volume (surrounding space) to substrate volume between 0.01 and 1.0, and an air volume (surrounding space) to substrate area is between 0.5 and 5 cc/cm, wherein the CO.sub.2 concentration is held above 3%, the relative humidity is held above 40% and the O.sub.2 concentration is held below 20% in steady state conditions to produce leather-like mycelium without fruiting bodies.

The present disclosure provides a method for aseptically culturing fruiting bodies of Antrodia cinnamomea, including: inoculating Antrodia cinnamomea strains in a sterilized agar medium, and performing a subculture to generate secondary mycelia and fruiting bodies of Antrodia cinnamomea.