The present disclosure provides a cultivation method of morels and belongs to the technical field of cultivation of edible fungi. After nutrient contents that can be metabolized and utilized by morel mycelia are scientifically matched, sterilization treatment of exogenous nutrients is eliminated, and the exogenous nutrients are directly compressed for forming. Formed exogenous nutrient blocks and spawn is sowed and covered with soil, so as to realize a potential difference between rich and poor nutrients in a physical space. When the spawn germinate to form a mycelium network, the mycelia will enter a nutrient-rich exogenous nutrient area, secrete various extracellular enzymes to decompose and utilize these exogenous nutrient blocks, and transport them to a nutrient-poor mycelium network for storage, so as to complete nutrient assimilation and absorption, and reserve energy for later sexual reproduction.

The present disclosure provides a cultivation method of morels and belongs to the technical field of cultivation of edible fungi. After nutrient contents that can be metabolized and utilized by morel mycelia are scientifically matched, sterilization treatment of exogenous nutrients is eliminated, and the exogenous nutrients are directly compressed for forming. Formed exogenous nutrient blocks and spawn is sowed and covered with soil, so as to realize a potential difference between rich and poor nutrients in a physical space. When the spawn germinate to form a mycelium network, the mycelia will enter a nutrient-rich exogenous nutrient area, secrete various extracellular enzymes to decompose and utilize these exogenous nutrient blocks, and transport them to a nutrient-poor mycelium network for storage, so as to complete nutrient assimilation and absorption, and reserve energy for later sexual reproduction.

The invention relates to a process for preparing a solid lignocellulosic material (1), referred to as a composite material, seeded with at least one organism (2), referred to as a filamentous fungus, which is a mycelium-forming multicellular eukaryote, in which: at least one solid lignocellulosic material (3) impregnated with an aqueous composition (5) is subjected to a treatment (6), known as a thermomechanical treatment, in which said at least one impregnated lignocellulosic material (3) is subjected to a succession of mechanical compression, expansion and shearing phases by blending at least one solid lignocellulosic material (4) of said at least one impregnated lignocellulosic material (3), in contact with the aqueous composition (5); said at least one impregnated lignocellulosic material (3) is brought to a temperature above 50 C.;
whereby a composition (7), referred to as a hydrated composition, comprising a solid lignocellulosic material (8), referred to as hydrated lignocellulosic material, the specific surface area and moisture content of which are increased relative to the specific surface area and moisture content of said at least one starting lignocellulosic material (4), is formed, said hydrated lignocellulosic material (8) being suitable for being colonized by said at least one filamentous fungus (2); and then a composition, known as a fungal composition (9), comprising said filamentous fungus (2) is added to said hydrated composition (7) during blending;
in which process the successive steps are performed continuously in at least one twin-screw extruder (10).

The invention relates to a process for preparing a solid lignocellulosic material (1), referred to as a composite material, seeded with at least one organism (2), referred to as a filamentous fungus, which is a mycelium-forming multicellular eukaryote, in which: at least one solid lignocellulosic material (3) impregnated with an aqueous composition (5) is subjected to a treatment (6), known as a thermomechanical treatment, in which said at least one impregnated lignocellulosic material (3) is subjected to a succession of mechanical compression, expansion and shearing phases by blending at least one solid lignocellulosic material (4) of said at least one impregnated lignocellulosic material (3), in contact with the aqueous composition (5); said at least one impregnated lignocellulosic material (3) is brought to a temperature above 50 C.;
whereby a composition (7), referred to as a hydrated composition, comprising a solid lignocellulosic material (8), referred to as hydrated lignocellulosic material, the specific surface area and moisture content of which are increased relative to the specific surface area and moisture content of said at least one starting lignocellulosic material (4), is formed, said hydrated lignocellulosic material (8) being suitable for being colonized by said at least one filamentous fungus (2); and then a composition, known as a fungal composition (9), comprising said filamentous fungus (2) is added to said hydrated composition (7) during blending;
in which process the successive steps are performed continuously in at least one twin-screw extruder (10).

Textile compositions comprising at least one filamentous fungus are disclosed, as are methods for making and using such textile compositions. Embodiments of the textile compositions generally include at least one of a plasticizer, a polymer, and a crosslinker, in addition to the filamentous fungus. The disclosed textile compositions are particularly useful as analogs or substitutes for conventional textile compositions, including but not limited to leather.

Methods are disclosed for generating aerial mycelium, such as mycological material comprising solely mycelia from depleted substrate or depleted and rejuvenated substrate. Alternatively, methods are disclosed for generating mycelia and mushrooms from depleted substrate or depleted and rejuvenated substrate. Alternative methods are disclosed for generating differentiated mycelium materials using depleted substrate or depleted and rejuvenated substrate. The mycelia products that are generated can be used in the food industry (e.g., as a meat analog) and in other industries, such as textiles, packaging, and others. The present invention provides systems and methods for generating mycelia that are repeatable and energy efficient, while providing consistently high quality and quantity mycelium-based products.

An edible meat substitute product includes a fibrous mycelium mass in a range of 10 wt % to 100 wt %. The fibrous mycelium mass has a protein content greater than 40 wt % of a dry mass of the fibrous mycelium mass. The edible meat substitute product includes a water content in a range of 0 w % to 90 wt %.

A method of growing fungal mycelium and forming edible food products includes growing fungal cells in a growth media such that the fungal cells produce mycelium. The growth media includes a sugar, a nitrogen-containing compound, and a phosphate-containing compound. The mycelium is separated from the growth media. The mycelium is concentrated to obtain a fibrous mycelium mass having a protein content of greater than 40 wt % of a dry mass of the mycelium. Food additives can be added to the fibrous mycelium mass to produce a biomass. The biomass can be formed into an edible food product. The biomass can be formed into an edible meat substitute product.

A method of growing fungal mycelium and forming edible food products includes growing fungal cells in a growth media such that the fungal cells produce mycelium. The growth media includes a sugar, a nitrogen-containing compound, and a phosphate-containing compound. The mycelium is separated from the growth media. The mycelium is concentrated to obtain a fibrous mycelium mass having a protein content of greater than 40 wt % of a dry mass of the mycelium. Food additives can be added to the fibrous mycelium mass to produce a biomass. The biomass can be formed into an edible food product. The biomass can be formed into an edible meat substitute product.

The present invention relates to a device for growing mushrooms, comprising a shelving, arranged for supporting beds for holding compost, said beds for holding compost wherein the beds are placed at a mutual distance above each other characterized in that the beds are movable between at least a first position, wherein a second bed supported above a first bed at least partially impedes the accessibility of the first bed in a direction perpendicular to the plane in which the first bed extends and a second position in which at least a larger part of the first bed is free approachable from a direction perpendicular to the plane in which the first bed extends than in the first position.