An edible meat substitute product includes a fibrous mycelium mass in a range of 10 wt % to 100 wt %. The fibrous mycelium mass has a protein content greater than 40 wt % of a dry mass of the fibrous mycelium mass. The edible meat substitute product includes a water content in a range of 0 w % to 90 wt %.

A monokaryotic mycelium sheet producing system for creating a sheet of monokaryotic mycelial material. The mycelium sheet producing system includes a culture unit, a spore stock unit, a plating unit, a section unit, a sub-plating unit, an expanding unit and a colonization unit. The culture unit prepares a monokaryon culture. The spore stock unit grows a plurality of fruit bodies in sterile laboratory conditions to create a spore stock. The plating unit performs a peroxide-based spore rescue and a plating process. The section unit is adaptable to section robust hyphae. The sub-plating unit sub-plates and expands the robust hyphae onto a spawn grain master. The expanding unit subsequently expands the spawn grain master into appropriate production of spawn volume. The colonization unit is adaptable to perform a subsequent colonization of mycelium substrate thereby creating a substantially defect free sheet of mycelium.

A monokaryotic mycelium sheet producing system for creating a sheet of monokaryotic mycelial material. The mycelium sheet producing system includes a culture unit, a spore stock unit, a plating unit, a section unit, a sub-plating unit, an expanding unit and a colonization unit. The culture unit prepares a monokaryon culture. The spore stock unit grows a plurality of fruit bodies in sterile laboratory conditions to create a spore stock. The plating unit performs a peroxide-based spore rescue and a plating process. The section unit is adaptable to section robust hyphae. The sub-plating unit sub-plates and expands the robust hyphae onto a spawn grain master. The expanding unit subsequently expands the spawn grain master into appropriate production of spawn volume. The colonization unit is adaptable to perform a subsequent colonization of mycelium substrate thereby creating a substantially defect free sheet of mycelium.

The process for producing mycelium biomaterial provides fresh oxygen to the growing mycelium biomaterial while removing waste heat and waste carbon dioxide by forced aeration through large volumes of material. In a first phase of fungal expansion, humidified air at a programmed temperature is passed upwardly and through a fungal inoculated substrate of discrete particles to allow the fungal inoculum to expand and dominate the substrate. Nutrient is added to the inoculated mixture and a second phase of fungal expansion is performed wherein humidified air at a programmed temperature is passed upwardly and through the nutrient enriched fungal inoculated substrate to allow the fungal inoculum to bond the discrete particles into a self-supporting biocomposite.

The process and apparatus of the invention allows for the processing of grown materials bound by mycelium at depths of greater than 6″ and particularly in the range of from 24″ to 28″.

The present disclosure provides a method for aseptically culturing fruiting bodies of Antrodia cinnamomea, including: inoculating Antrodia cinnamomea strains in a sterilized agar medium, and performing a subculture to generate secondary mycelia and fruiting bodies of Antrodia cinnamomea.

The present invention is a method of wowing mushrooms, preferably but not necessarily) Basiomycetes mushrooms, on a substrate that includes unrefined, live or dried cannabis plants (whole or particulated) preferably those cannabis, strains high in CBD and low in THC or containing a preferred ratio thereof, as part or all of the growth medium for the mushrooms.

Disclosed herein is an industrial scale process of cultivating cultivating Ganoderma lucidum mycelia. As high as 15 Kg dried Ganoderma lucidum mycelia may be produced from about 300 liters liquid culture every 30 days, and the resulting dried Ganoderma lucidum mycelia are rich in triterpenoids that include at least ganoderic acid S (GAS), ganoderic acid T (GAT), ganoderic acid Me (GAMe), ganoderic acid R (GAR), and ganodermic acid S (GMAS).

Provided is a method of culturing ganoderma lucidum mycelia in which the content of ganodermanondiol serving as an inhibitor to melanin biosynthesis is enhanced to improve skin whitening function.

For indoor mushroom cultivation, a method of irrigating the mushrooms includes drip irrigation pipes in the bed. The drip irrigation pipes can be disposed in a casing layer that overlies a substrate layer, and irrigation can be according to measurements taken in the environment outside of the bed.

A growth medium for fungus is obtained by: (a) treating a mixture of lignocellulosic biomass and water with at least one oxidizing agent and steam at a temperature in a range of from about 130 C to about 220 C for a period from about 5 seconds to about 10 hours; (b) periodically measuring a pH of the mixture for substantially an entire duration of the treating step; and (c) as necessary, based on the pH of the mixture measured in step (b), adjusting the pH of the mixture into a range of from about pH 4.5 to about pH 7.5 by adding abase to the mixture. A method for producing the growth medium for fungi and a method for cultivating fungi are also provided.