A method of producing a microparticle includes providing a mould assembly, which comprises two moulds that comprise an upper mould and a lower mould, positioning the mould assembly in a closed position, wherein the two moulds define a micro-cavity to exert pressure on a moulding material within the micro-cavity to form the moulding material into a microparticle, and positioning the mould assembly in an open position, wherein the microparticle adheres to one of the two moulds of the mould assembly.

This invention relates to compounds useful for treating fungal infections, more specifically topical treatment of onychomycosis and/or cutaneous fungal infections. This invention is directed to compounds that are active against fungi and have properties that allow the compound, when placed in contact with a patient, to reach the particular part of the skin, nail, hair, claw or hoof infected by the fungus. In particular the present compounds have physiochemical properties that facilitate penetration of the nail plate.

A method of producing a microparticle includes providing a mould assembly, which comprises two moulds that comprise an upper mould and a lower mould, positioning the mould assembly in a closed position, wherein the two moulds define a micro-cavity to exert pressure on a moulding material within the micro-cavity to form the moulding material within the micro-cavity to form the moulding material into a microparticle, and positioning the mould assembly in an open position, wherein the microparticle adheres to one of the two moulds assembly.

Self-actuating articles including, for example, self-actuating needles and/or self-actuating biopsy punches, are generally provided. Advantageously, the self-actuating articles described herein may be useful as a general platform for delivery of a wide variety of pharmaceutical drugs that are typically delivered via injection directly into tissue due to degradation in the GI tract. The self-actuating articles described herein may also be used to deliver sensors and/or take biopsies without the need for an endoscopy. In some embodiments, the article comprises a spring (e.g., a coil spring, a beam, a material having particular mechanical recovery characteristics). Those of ordinary skill in the art would understand that the term spring is not intended to be limited to coil springs, but generally encompass any reversibly compressive material and/or component which, after releasing an applied compressive force on the material/component, the material/component substantially returns to an uncompressed length of the material/component (e.g., the within 95% of the length of the material/component prior to compression).

Methods of treating cancer in a subject by administering a therapeutically effective amount of a polycationic polymer to the subject are provided. Methods of treating cancer in a subject by contacting a bodily fluid from a subject with a polycationic polymer are also provided. Methods of detecting cancer or metastasis of cancer by obtaining a sample from a subject and determining the level of circulating free DNA (cfDNA), cfRNA, inorganic polyphosphates or the number of exosomes or nucleosomes in the sample are also provided.