Provided herein are methods for treating a patient with ADHD and anxiety, comprising administering to the patient a therapeutically effective amount of psilocybin. The disclosure also provides methods for treating ADD and reducing symptoms of Tourette Syndrome using psilocybin. Additionally, methods of cultivation, extraction, and purification of psilocybin mushrooms are described. The psilocybin is obtained from mushroom species such as Psilocybe cubensis, Psilocybe cyanescens, Psilocybe semilanceata, or Psilocybe azurescens. The methods involve tissue culture, agar inoculation, grain-to-grain transfer, bulk substrate inoculation, and fruiting. Extraction and purification steps include harvesting, drying, grinding, solvent extraction, filtration, evaporation, and cryogenic separation.

Provided are methods to produce an edible filamentous fungal biomass using an aqueous media which has a carbon source and a nitrogen source into which is inoculated filamentous fungal culture followed by culturing in a submerged fungal culture to produce an edible filamentous fungal biomass, wherein the fungal culture comprises Pleurotus spp. The culture may be grown to at least about 25 g/L (dry weight) with a productivity of at least 2.5 g/L/day (dry weight) during the culturing step. Also provided herein are compositions including an edible filamentous fungus.

Provided are methods to produce an edible filamentous fungal biomass using an aqueous media which has a carbon source and a nitrogen source into which is inoculated filamentous fungal culture followed by culturing in a submerged fungal culture to produce an edible filamentous fungal biomass, wherein the fungal culture comprises Pleurotus spp. The culture may be grown to at least about 25 g/L (dry weight) with a productivity of at least 2.5 g/L/day (dry weight) during the culturing step. Also provided herein are compositions including an edible filamentous fungus.

A mycelium-based biocomposite comprising mycelium and a substrate including one or more textiles wherein the mycelium is integrated into the substrate and a method for making a mycelium using one or more textiles as a substrate. Growth of mycelial cells into networks throughout the substrate provide the biocomposite with improved morphological and structural properties.

A mycelium-based biocomposite comprising mycelium and a substrate including one or more textiles wherein the mycelium is integrated into the substrate and a method for making a mycelium using one or more textiles as a substrate. Growth of mycelial cells into networks throughout the substrate provide the biocomposite with improved morphological and structural properties.

Example mixers and methods for using mixers for cultivating mushrooms are disclosed. An example single stage mixer for cultivating mushrooms includes a mixing container defining an internal mixing chamber, a lid coupled to the mixing container, the lid configured to shift between a sealed configuration and an open configuration. The mixing container also includes a rotatable agitator coupled to the mixing container, a water supply member positioned within the internal mixing chamber and a heating element attached to the mixing container.

Example mixers and methods for using mixers for cultivating mushrooms are disclosed. An example single stage mixer for cultivating mushrooms includes a mixing container defining an internal mixing chamber, a lid coupled to the mixing container, the lid configured to shift between a sealed configuration and an open configuration. The mixing container also includes a rotatable agitator coupled to the mixing container, a water supply member positioned within the internal mixing chamber and a heating element attached to the mixing container.