Mesenchymal stem cells may be culture for a long period, without using any special apparatus, equipment and the like, in a medium in which seven kinds of nonessential amino acids of glycine, alanine, serine, proline, asparagine, aspartic acid, and glutamic acid are reduced.

Mesenchymal stem cells may be culture for a long period, without using any special apparatus, equipment and the like, in a medium in which seven kinds of nonessential amino acids of glycine, alanine, serine, proline, asparagine, aspartic acid, and glutamic acid are reduced.

The invention refers to a process for the production of a continuous cell line of simile embryo stem cells, such as Amblyomma sculptum (Acari: Ixodidae), known as line IBU/ASE-16 (access number with CNCM: 1-5000) and its uses. More specifically, the invention refers to a process for the production of the line IBU/ASE-16 and their use for obtaining extracts for the production of vaccines and candidate recombinant proteins for biopharmaceuticals and acaricides, production of diagnostic kits for the detection of antigens and/or antibodies for animal and human use, obtaining clones for use in genotyping and use as a substrate for the isolation and culture of pathogens.

The invention refers to a process for the production of a continuous cell line of simile embryo stem cells, such as Amblyomma sculptum (Acari: Ixodidae), known as line IBU/ASE-16 (access number with CNCM: 1-5000) and its uses. More specifically, the invention refers to a process for the production of the line IBU/ASE-16 and their use for obtaining extracts for the production of vaccines and candidate recombinant proteins for biopharmaceuticals and acaricides, production of diagnostic kits for the detection of antigens and/or antibodies for animal and human use, obtaining clones for use in genotyping and use as a substrate for the isolation and culture of pathogens.

Anti-viral compounds with low cytotoxicity are identified from screening of products found in Red Sea sponges, including the sponge Stylissa carteri. The identified compounds can be brominated pyrrole-2-aminoimidazole alkaloids and derivatives thereof. Specific examples of identified compounds include oroidin, hymenialdisine, and debromohymenialdisine, as well as derivatives thereof. The compounds also can be useful scaffolds or pharmacores for further chemical modification and derivatization. Selected compounds, particularly oroidin, show selective anti-viral HIV-1 activity coupled with reduced cytotoxicity. The compounds can function as HIV reverse-transcriptase inhibitors, and molecular modeling can be used to confirm inhibition.

The present invention discloses a shredded-meat-shaped pet food which consists of the following raw materials in percentage by mass: 65 to 95% of plant-based protein, 0 to 20% of starch, 0 to 15% of animal raw materials, 1 to 7% of vegetables, 1 to 5% of fruits, 0.5 to 3% of beer yeast, 0.02 to 0.05% of potassium sorbate, 0.1 to 0.7% of salt, 0.3 to 0.5% of essences, 0.01 to 0.05% of phosphate and 0.02 to 0.03% of mixed vitamins. According to the technical solution of the present invention, the plant-based protein, meat, vegetables and fruits are prepared into the shredded-meat-shaped pet food by a mechanical puffing extrusion technology. The shredded-meat-shaped pet food in the technical solution of the present invention is a product having a fibrous shredded-meat shape, and thus has a very good sensory effect and palatability effect. As more and more people keep pets, such shredded-meat-shaped pet food has a broad development prospect and needs to be developed urgently to fill the market gap.

The present invention discloses a shredded-meat-shaped pet food which consists of the following raw materials in percentage by mass: 65 to 95% of plant-based protein, 0 to 20% of starch, 0 to 15% of animal raw materials, 1 to 7% of vegetables, 1 to 5% of fruits, 0.5 to 3% of beer yeast, 0.02 to 0.05% of potassium sorbate, 0.1 to 0.7% of salt, 0.3 to 0.5% of essences, 0.01 to 0.05% of phosphate and 0.02 to 0.03% of mixed vitamins. According to the technical solution of the present invention, the plant-based protein, meat, vegetables and fruits are prepared into the shredded-meat-shaped pet food by a mechanical puffing extrusion technology. The shredded-meat-shaped pet food in the technical solution of the present invention is a product having a fibrous shredded-meat shape, and thus has a very good sensory effect and palatability effect. As more and more people keep pets, such shredded-meat-shaped pet food has a broad development prospect and needs to be developed urgently to fill the market gap.

Anti-viral compounds with low cytotoxicity are identified from screening of products found in Red Sea sponges, including the sponge Stylissa carteri. The identified compounds can be brominated pyrrole-2-aminoimidazole alkaloids and derivatives thereof. Specific examples of identified compounds include oroidin, hymenialdisine, and debromohymenialdisine, as well as derivatives thereof. The compounds also can be useful scaffolds or pharmacores for further chemical modification and derivatization. Selected compounds, particularly oroidin, show selective anti-viral HIV-1 activity coupled with reduced cytotoxicity. The compounds can function as HIV reverse-transcriptase inhibitors, and molecular modeling can be used to confirm inhibition.

Methods and protocols of harvesting and processing of Porifera species, specifically sponges, and more specifically fresh water species of Genus Spongilla; testing and storing of the processed Spongilla powder; and manufacturing and packaging of Spongilla-based therapeutic compositions for treating and preventing skin diseases are disclosed. Treatable skin conditions and diseases include without limitation acne vulgaris, rosacea, seborrheic dermatitis, psoriasis, photo-aging and actinic keratosis, acne vulgaris, psoriasis, photo-aging, wounds, scars, and eczema (atopic dermatitis).

Methods and protocols of harvesting and processing of Porifera species, specifically sponges, and more specifically fresh water species of Genus Spongilla; testing and storing of the processed Spongilla powder; and manufacturing and packaging of Spongilla-based therapeutic compositions for treating and preventing skin diseases are disclosed. Treatable skin conditions and diseases include without limitation acne vulgaris, rosacea, seborrheic dermatitis, psoriasis, photo-aging and actinic keratosis, acne vulgaris, psoriasis, photo-aging, wounds, scars, and eczema (atopic dermatitis).