Described herein are processes and compositions for ultrasound-triggered liposome payload release, including a process for gelation and a process for enzyme catalysis.

The present invention provides methods for preparing acoustically-sensitive microbubbles. The method includes the steps of: i) preparing a first surfactant solution comprising a first micelle-forming surfactant at a concentration above the critical micelle concentration (CMC); ii) adding one or more pharmaceutical compounds in a solvent to the first surfactant solution, thereby loading the micelles with the one or more pharmaceutical compounds; iii) preparing a second surfactant solution comprising a second surfactant, wherein the second surfactant comprises one or more matrix forming surfactants; iv) adding heat to the second surfactant solution to melt the surfactant and allowing the mixture to cool under rapid stirring; v) combining the second surfactant solution with the loaded micelles; vi) purging the surfactant mixture with a purging gas; vii) agitating the purged mixture under a constant stream of the purging gas; and, viii) separating the formed microbubbles by size.

A novel means for safely and efficiently introducing a target substance such as nucleic acid, protein, or the like into cells (excluding immune cells) is provided by the present invention. Specifically, a system for delivering a target substance into a cell (excluding immune cells), including ultrafine bubble water or ultrafine bubble aqueous solution containing ultrafine bubbles with an average diameter of not more than 200 nm and not containing phospholipid, and an ultrasound generator in combination; a method for increasing the delivery of a nucleic acid, a protein or a low-molecular-weight compound into a cell (excluding immune cells) by using the ultrafine bubble water, etc.; and the like are provided.

The present invention relates to a cancer cell-targeted drug delivery carrier, a composition for promoting photo-thermal treatment effects, and the like, which contain M1 macrophages as an active ingredient. The drug delivery carrier of the present invention uses the M1 macrophages mobility to tumor cells and the M1 macrophage penetrability into tumors, and can deliver drugs specifically to tumor and cancer tissues only, and, when performing photo-thermal treatment by loading M1 macrophages with a photosensitive material, can significantly increase the effects, and thus is expected to be effectively used for promoting cancer treatment effects.

In various aspects, the present disclosure provides methods, compositions, and kits for treating a target tissue with one or more active agents. In embodiments, delivering an active agent to a target tissue comprises administration of microbubbles and ultrasound.

The method of treating tumors and causing full regrowth and regeneration of tissue provides for the treatment and removal of tumors through application of a static magnetic field to flesh containing a tumor, as well as quickly and effectively healing, regenerating and regrowing the tissue following treatment of the tumor. At least two magnets are positioned within a treatment region adjacent to the tumor. Flesh containing a tumor is clamped between a first annular magnet and a second annular magnet, such that the flesh is sandwiched therebetween, with each of the first and second annular magnets surrounding the tumor. Treatment of the tumor causes the tumor to become necrotic and separate from the flesh. The application of the static magnetic field is further found to effect enhanced healing, regeneration and regrowth of the tumor-free tissue.

A method for early stage pathology detection, location, imaging, evaluation, and treatment of cells and/or extracellular vesicles in the circulation.

A microbubble composition, comprising: a plurality of microbubbles, a microbubble comprising a noble gas and/or perfluorocarbon encapsulated within a shell that comprises one or more of a lipid, a protein, or a polymer, and a microbubble optionally defining a cross-sectional dimension in the range of from about 0.5 to about 20 micrometers. A method, comprising forming a composition according to the present disclosure. A method, comprising administering a microbubble composition according to the present disclosure to a subject, the composition optionally comprising echogenic phospholipid microbubbles. A method, comprising: (a) identifying, with the application of energy, the location of a microbubble composition according to the present disclosure, the energy optionally being ultrasound, (b) controllably effecting rupture of microbubbles of a microbubble composition of the present disclosure, the rupture optionally being effected by application of ultrasound, or both (a) and (b).

Disclosed are methods comprising: administering a drug-loaded nanoparticle to a subject; and exposing a target site of the subject to an alternating electric field for a period of time, the alternating electric field having a frequency and field strength, and wherein the alternating electric field triggers the release of the drug from the drug-loaded nanoparticle at the target site of the subject. Disclosed are methods comprising: administering a drug-loaded nanoparticle to a subject; and exposing a target site of the subject to an alternating electric field for a period of time, the alternating electric field having a frequency and field strength, wherein the alternating electric field triggers the release of the drug from the drug-loaded nanoparticle at the target site of the subject, thereby decreasing non-target site specific release of the drug. Disclosed are methods of treating cancer in a subject comprising administering a drug-loaded nanoparticle to a subject having cancer; and exposing a target site of the subject to an alternating electric field for a period of time, the alternating electric field having a frequency and field strength, wherein the target site comprises a cancer cell; wherein the alternating electric field triggers the release of the drug from the drug-loaded nanoparticle at the target site of the subject; wherein the drug kills the cancer cell. Disclosed are methods of killing cancer cells comprising administering a drug-loaded nanoparticle to a subject; and exposing a target site of the subject to an alternating electric field for a period of time, the alternating electric field having a frequency and field strength, wherein the alternating electric field triggers the release of the drug from the drug-loaded nanoparticle at the target site of the subject, wherein the target site comprises cancer cells, wherein the drug kills the cancer cells.

This invention related to manufactured microbubbles, as well as methods of using manufactured microbubbles, for example, in medicinal applications. The invention pertains to the physical structure and materials of the microbubbles, as well as to methods for manufacturing microbubbles, methods for targeting microbubbles for specific medicinal applications, and methods for delivering microbubbles in medical treatment.