In one aspect, compositions are described herein. A composition described herein comprises a nanoparticle, a therapeutic species, and a linker joining the nanoparticle to the therapeutic species. The linker joining the nanoparticle to the therapeutic species comprises a Diels-Alder cyclo-addition reaction product. Additionally, in some embodiments, the nanoparticle is a magnetic nanoparticle.

In some aspects, the present disclosure provides mechanosensitive vesicles, which are light sensitive for delivery of a guest molecule. In some embodiments, these vesicles include mechanosensitive phospholipids which have been coated with a noble metal coating.

This invention discloses methods and composition to form biodegradable polymer implant arrays in the live tissue. Artificial cavities are created in the live tissue by using laser ablation, oscillating needle, microneedle array and other methods. The cavities are then filled with biodegradable polymer solution. The solvent in the polymer solution is dissipated in the tissue to form a biodegradable polymer implant in artificial cavities. The cavities and implants formed are arranged to form of an array of implants. The biodegradable polymer in the cavity can also be loaded with drug to form biodegradable drug delivery array in the live tissue.

The present invention relates to an ultrasound-induced drug delivery system using a drug carrier containing a plurality of nanobubbles and a high concentration of a drug in one microcapsule, and specifically is directed to a method for preparing a drug delivery system having a high concentration of a drug and a plurality of nanobubbles encapsulated therein, by generating the nanobubbles in an oil into which the drug is dissolved using a nanobubble generator, and then microencapsulating them; and an ultrasound-induced drug delivery system using the same.

The drug delivery system using the nanobubbles according to the present invention is prepared in the form of microcapsules in which both the drug and the nanobubbles are encapsulated, and, in particular, has an effect of maximizing drug delivery efficiency as the nanobubbles collapse or aggregate when the ultrasound is applied to the drug delivery system.

Further, since the drug delivery system of the present invention contains a plurality of nanobubbles within the microcapsules, it can also be used as a contrast agent. The drug delivery system prepared according to the method described in this specification and having both the drug and the nanobubbles encapsulated therein has a feature that can simultaneously perform in vivo diagnosis and treatment.

Compositions, kits, and systems are disclosed that include calreticulin gene delivering nanoparticles that can be used alone or in combination with focused ultrasound to induce immunogenic cell death in tumors. Also disclosed are methods of producing and using the compositions, kits, and systems.

The present invention relates to a microbubble complex comprising a microbubble having an outer shell comprising a mixture of native and denatured albumin encapsulating a perfluorocarbon gas, a therapeutic agent, a bifunctional linker having one end attached to the therapeutic agent and the other attached to a ligand and wherein the ligand is bound to the other shell of the microbubble through hydrophobic interactions. Also included are methods for delivering the aforementioned microbubble complex to a tissue target.

This invention relates to photoswitchable inhibitors of histone deacetylases and methods of using the same.

A method of forming an implant in tissue can include: providing a polymer solution having an effective amount of polymer dissolved in a biocompatible, water-soluble organic solvent; injecting a plurality of droplets of the polymer solution in the tissue; fusing the injected droplets together; dissipating the biocompatible, water-soluble organic solvent in the tissue; and precipitating the polymer from the injected polymer solution so as to form the implant.

The present invention provides a platform for the delivery of small molecule drugs or contrast agents to joints and other soft tissues. The platform enables penetration of the drug through the full thickness of cartilage and long intra-cartilage residence time by leveraging electrostatic interactions between the cationic platform and the anionic cartilage matrix. Described herein are compounds and complexes that fit this platform. Also provided are methods of treating a joint disease with the compounds and complexes of the invention, and methods of imaging joints and other soft tissue.

A method of killing cells of a targeted cell type in a patient body that utilizes nanoparticles having a first portion, which when exposed to a target portion of a targeted cell type, binds to the target portion and a second portion, joined to the first portion, and comprised of a low resistivity material. The nanoparticles are introduced into a contact area where they contact cells of the targeted cell type. Contemporaneously, the contact area is exposed to a varying magnetic field of insufficient strength to increase the temperature of any part of the patient body by more than ten degrees Celsius, but which creates a current at the nanoparticles sufficient to disrupt function of the targeted cell type.