The present invention is directed to a system and method for photoeradication of microorganisms from a target. The method includes the step of obtaining test data for a plurality of experiments each of which comprises irradiating test microorganisms with a plurality of light pulses having a wavelength that ranges from 380 nm to 500 nm. The light pulses have a plurality of pulse parameters (peak irradiance, pulse duration, and off time between adjacent light pulses) and are provided at a radiant exposure that ranges from 0.5 J/cm.sup.2 to 60 J/cm.sup.2 during each of a plurality of irradiation sessions. The test data comprises a survival rate for the test microorganisms after irradiation with the light pulses. The method also includes the step of analyzing the test data to identify the pulse parameters for the light pulses and the radiant exposure for each of the irradiation sessions that result in a desired survival rate for the test microorganisms. The method further includes the step of irradiating the microorganisms of the target with light pulses having the identified pulse parameters at the identified radiant exposure for each of the irradiation sessions so as to photoeradicate all or a portion of the microorganisms.

An example antimicrobial treatment system includes an illumination system configured to deliver illumination that activates a photosensitizing agent applied to a cornea. The system also includes a controller configured to control the illumination system. The controller detects an ulcerative region on a cornea and causes the illumination system to deliver the illumination to activate the photosensitizing agent applied to the ulcerative region according to a set of parameters for treating the ulcerative region. The illumination is restricted to the ulcerative region, and activation of the photosensitizing agent in the ulcerative region generates an antimicrobial effect.

Disclosed are a diglycosylated benzophenoxazine photosensitizer, and a preparation method and use thereof. The present invention greatly improves the enriched concentration of the photosensitizer in tumor cells by taking full advantage of the enhanced uptake and enhanced glycolysis of carbohydrates by tumor cells and the glycosylation of a selenium-containing benzophenoxazine compound, thereby improving the targeting of a diglycosylated benzophenoxazine photosensitizer involved in the present invention in the treatment of cutaneous tumors and also significantly decreasing the toxic and side effects of the photodynamic therapy. The present invention can efficiently and rapidly inhibit the proliferation of cells of cutaneous squamous cell carcinoma and essentially cause no damage to normal cells.

The present disclosure discloses preparation and application of a novel multifunctional nanocomposite material with new photosensitizer, and belongs to the technical field of photodynamic therapy and the field of biomedicine. The photosensitizer multifunctional nanocomposite material provided by the present disclosure is prepared by self-assembly of cercosporin and an acid-sensitive copolymer multifunctional material with liver tumor cell targeting ability and traceability, wherein the acid-sensitive copolymer multifunctional material can be a copolymer of poly(N,N-dimethylaminoethyl methacrylate) and poly-3-azido-2-hydroxypropyl methacrylate covalently linked by galactose-modified rhodamine B. The photosensitizer multifunctional nanocomposite material disclosed by the present disclosure can specifically recognize liver tumor cells and be endocytosed into the cells through galactose-asialoglycoprotein receptor interaction, and can trigger the release of the photosensitizer cercosporin under acidic pH conditions to exert photodynamic therapy efficiency. The novel photosensitizer multifunctional nanocomposite material has a good application prospect in targeted photodynamic therapy of tumor cells.

The present invention is directed to compositions and methods targeting cells in a subject harboring conditions or at risk for conditions that would benefit from gas-based diagnostic and therapy. The present invention relates to the use of fluorochemical compositions and methods of delivery that result in retention of the fluorochemical composition and any bioactive agent, including gaseous substances, delivered in combination with the fluorochemical composition.

The present invention provides a hydrogel for comprising a biodegradable polyphosphazene polymer, a radiation-sensitive diselenide cross-linker; and one or more payloads releasably loaded within the hydrogel. The present invention further provides methods for radiosensitizing target tissues such as tumors and providing sustained delivery of therapeutics triggered by irradiation. In another aspect, the present invention provides a method that includes: introducing the hydrogel, as describes herein, adjacent to malignant or marginal tissue; and administering radiation to the hydrogel, thereby disrupting the selenocystamine cross-linkers and releasing the one or more payloads.

Methods and systems for radiation therapy involve administering a payload/combination of biocompatible high-Z and semiconductor NPs to tissue, such as a tumor or an eye. Ionizing radiation may be directed towards the payload, and ionized electrons generate Cerenkov radiation (CR). The CR interacts with semiconductor NPs to produce chemical species that are damaging to cells. The payload may be administered via injection or via a radiotherapy (RT) device that includes NPs in a biodegradable polymer matrix. Biodegradation of the polymer matrix, which results in release of its payload, may be remotely activated using, for example, electromagnetic or sound waves. The payload may include one or more immunologic adjuvants capable of promoting an immunologic response at remote sites (such as a metastatic tumors) that are separate from the site at which the NPs and adjuvants were administered.

The present invention relates to an apparatus (1) for use in irradiation therapy, comprising an ionization module (2) adapted to emit ionization irradiation, and a power source (4) and a control unit (5) to provide a user interface. The apparatus is characterized in that the apparatus comprises an UV module (3) adapted to emit UVA, UVB and/or UVC irradiation (9), whereby the ionization module and the UV module emit irradiation simultaneously or alternately, and the ionization module emits irradiation (8) at a wave length at least below 100 nm. The invention also relates to a use of the apparatus for radiating an object (7) and use of the apparatus and method for treatment of a mammal (7). A detector may measure and/or create an image of the irradiation (6).

The present invention relates to a cobalt-nitrosyl complex having photocleavable ligands. The cobalt-nitrosyl complex provided in one aspect of the present invention is adept in the delivery of NO, with exquisite temporal control using light, without gene editing. In addition, the complex provided in one aspect of the present invention is excellent in biocompatibility because it is chemically stable, non-toxic at cell level, and non-perturbative in cellular environments.

Disclosed are engineered particles comprising a photosensitizer and methods for treating cancer comprising administering the engineered particles and tumor-specific T cells to a subject, wherein the photosensitizer is stimulated by light comprising a wavelength that excites the photosensitizer.